Analyzing the Clinical Potential of Stromal Vascular Fraction: A Comprehensive Literature Review

Abstract

Background: Regenerative medicine is evolving with discoveries like the stromal vascular fraction (SVF), a diverse cell group from adipose tissue with therapeutic promise. Originating from fat cell metabolism studies in the 1960s, SVF’s versatility was recognized after demonstrating multipotency. Comprising of cells like pericytes, smooth muscle cells, and, notably, adipose-derived stem cells (ADSCs), SVF offers tissue regeneration and repair through the differentiation and secretion of growth factors. Its therapeutic efficacy is due to these cells’ synergistic action, prompting extensive research. Methods: This review analyzed the relevant literature on SVF, covering its composition, action mechanisms, clinical applications, and future directions. An extensive literature search from January 2018 to June 2023 was conducted across databases like PubMed, Embase, etc., using specific keywords. Results: The systematic literature search yielded a total of 473 articles. Sixteen articles met the inclusion criteria and were included in the review. This rigorous methodology provides a framework for a thorough and systematic analysis of the existing literature on SVF, offering robust insights into the potential of this important cell population in regenerative medicine. Conclusions: Our review reveals the potential of SVF, a heterogeneous cell mixture, as a powerful tool in regenerative medicine. SVF has demonstrated therapeutic efficacy and safety across disciplines, improving pain, tissue regeneration, graft survival, and wound healing while exhibiting immunomodulatory and anti-inflammatory properties.

Autologization of Exosomes with Deparenchymized Adipose Tissue: An Innovative Approach for Regenerative Medicine and Surgery

“…The Adinizer, a patented double-sided ultrasharp blade system, has been used in many different studies in the literature to obtain stromal cells from fat tissue and to apply different sizes to different anatomical areas in fat tissue grafts, and successful results have been reported. [11][12][13][14][15][16][17][18][19] The process of mechanically obtaining stromal cells using the Adinizer is called mechanical stromal cell transfer, whereas the creation of fat tissue in different sizes is called adjustable regenerative adipose transfer. 6 This approach has enabled the application of different protocols for different indications, and indication-based protocols have been presented in the literature for the first time.…”

Conservative Treatment in Avascular Necrosis of the Femoral Head: A Systematic Review

“…Key technologies employed include cellular therapies using bone marrow-derived mesenchymal stem cells (BMSCs), growth factor therapies, metallic implants, and advanced manufacturing techniques such as 3D bioprinting and nonoprinting for crafting ceramic and polymeric scaffolds. While these technologies remain experimental, their potential advantages and disadvantages are actively being explored to optimize regenerative outcomes for AVN [ 60 , 61 ].…”

Comparative Analysis of Stromal Vascular Fraction and Alternative Mechanisms in Bone Fracture Stimulation to Bridge the Gap between Nature and Technological Advancement: A Systematic Review

“…The steps of SVF separation can be summarized as (a) liposuction, (b) mechanical separation or faxination, (c) initial filtration, (d) washing, (e) final filtration, (f) SVF and adipose graft harvesting, and (g) cell counting and/or characterization (Table 7) [52,53,[61][62][63][64]. In the process of finding optimal bone regeneration strategies, the exploration of SVF has presented substantial insights.…”

“…Differentiation from stem cell concentrators used in spinal surgery: stem cell concentrators used in spinal surgery typically involve the concentration of bone marrow aspirate [63]. This aspirate predominantly contains hematopoietic stem cells (HSCs) and a smaller proportion of mesenchymal stem cells [51,64].…”