At present, the challenging issue of the compatibility between stab resistance and flexibility of materials frequently appears. Thus, this study proposes a novel method to enhance the comprehensive performance of the material matrix with stab resistance. Based on the stab-resistant mechanism analysis of the textile matrix, the influence of four factors on the performance of ultra-high molecular weight polyethylene (UHMWPE) knitted fabric was discussed. And, the optimal process conditions of material for achieving high stab resistance and high flexibility were obtained by the response surface method. A series of experiments proved that among all factors, the fabric structure had the greatest influence on the flexible stab-resistant knitted material. Following that, the thickness of the yarn also plays a significant role. Under the optimal process conditions, the stab peak force of the knitted material was promoted to 52.450 N, and the flexibility was enhanced to 93.6%. Meanwhile, through comparison with products that have undergone the same treatment, there was little difference in stab resistance but significantly improved flexibility. It achieves the initial stab resistance and comfortable wearing softness of the fabric through process optimization. This improvement in overall performance of the textile matrix enables further enhancement treatments.