Anandamide levels in human female reproductive tissues: Solid-phase extraction and measurement by ultraperformance liquid chromatography tandem mass spectrometry

Transient receptor potential vanilloid 1 is expressed in human cytotrophoblasts: Induction of cell apoptosis and impairment of syncytialization

“…Since TRPV1 activation induces both apoptosis and ROS/RNS generation, a tight control of endovanilloid signalling may be required for a successful pregnancy outcome. Moreover, AEA is an endogenous lipid that is physiologically present in the human placenta (Marczylo et al, 2010) and here we report that it induces hCT apoptosis through TRPV1, suggesting a role for the endovanilloid signalling in this cellular event.…”

The endocannabinoid anandamide affects the synthesis of human syncytiotrophoblast-related proteins

“…AEA is physiologically present in human placenta (Marczylo et al 2010) and its levels may be regulated in situ by AEA metabolic enzymes (Habayeb et al 2008;Trabucco et al 2009). Therefore, deregulations in AEA-mediated signalling may negatively impact the gestational outcome.…”

Quantitative analysis of anandamide and related acylethanolamides in human seminal plasma by ultra performance liquid chromatography tandem mass spectrometry

“…Processing times include the 1 h required for liquefaction. Times were based upon a reasonable time for sample handling by the Andrology Unit and transfer to our laboratories (2 h) and that significant ex vivo increases in AEA concentrations have been observed by 4 h in previously investigated tissues (blood, placenta, and foetal membranes) [15]. Initially the stability of NAEs in seminal plasma samples during storage at −80 • C was investigated in matched samples after a single freeze-and-thaw cycle.…”

“…Furthermore, AEA extraction from biofluids using silica chromatography, as used in these published studies, but not with C18 solid phase, greatly overestimate concentrations due to deuterium loss from internal standards [13]. Ex vivo processing times have a significant effect upon levels of AEA in some biofluids and tissues [14,15]. The aims of this study were therefore to develop and validate methods for sample handling, AEA extraction using C18 solid-phase extraction and quantification of N-acylethanolamides (NAEs) by UHPLC-ESI-MS/MS in human seminal plasma.…”