Anatomical and histochemical characterization of some highly medicinal plants as a tool for quality control

Abstract

Medicinal plants are richest source of chemical compounds that can be used to evolve novel drugs. According to World Health Organization (WHO), in developing countries more than 3.5 billion people relay on herbal drugs for their primary health care. In the present study, an attempt was carried out to authenticate some selected medicinal plants (Fagonia cretica L., Peganum harmala L., Tribulus terrestris L., Chrozophora tinctoria L. Raf. and Ricinus communis L.) from family Zygophyllaceae and Euphorbiaceae using light and scanning electron macroscopic techniques. Macroscopic evaluation and comparative anatomy (Light Microscopy) of the root and fruits revealed great diversity in macro and microscopic features. Scanning electron microscopy (SEM) of root powder showed non‐glandular trichomes, stellate trichomes, parenchyma cells and vessels. Fruits SEM exhibited non‐glandular trichomes, glandular trichomes, stellate trichomes, peltate trichomes and mesocarp cells. Both macroscopic and microscopic evaluation plays a crucial role in the correct substantiation and validation of novel sources. These findings can serve as an essential source of information to accomplish the authenticity and to appraise the quality and purity of the herbal drugs in accordance to WHO guidelines. These parameters can be used to distinguish the selected plants from their commonly used adulterants. Research Highlights In the current work, five plants (Fagonia cretica L., Peganum harmala L., Tribulus terrestris L., Chrozophora tinctoria L. Raf. and Ricinus communis L.) from Zygophyllaceae and Euphorbiaceae were investigated for macroscopy and microscopy (LM & SEM) for the first time. Macroscopic and microscopic evaluation disclosed great diversity in morphology and histology. Microscopy is the backbone of standardization process. Current study helped in correct identification and quality assurance of the plant materials. Their statistical investigation may possess great potency for plant taxonomists to further appraise the vegetative growth and tissue development which is requisite especially for fruit to enhance the yield of herbal drugs and their formulation. Further molecular studies, compounds isolation and characterization are required to deepen the knowledge of these herbal drugs.

Pharmacognostic Evaluation, Chemical Characterization, and Antibacterial Activity of Bassia indica (Wight) A.J. Scott

“…Therefore, the water conservation and food storage ability of different parts of plants help the plant survive in drought conditions. Similar anatomical features have also been observed in other plants growing in drought conditions [30,32,33] Moreover, these observations provide information about the physical characteristics of the plant that may affect its solubility [28]. Extraction with different solvents showed the maximum yield in the aqueous solution.…”

Microscopic characterization, TLC fingerprinting and optimization of total lipid content from Euphorbia neriifolia (L.) using response surface methodology

“…Similar types of trichomes were found in Euphorbia hamiltonii, Euphorbia prostrata, Euphorbia pulcherrima (Zahra et al, 2014), and Phlomis herba-venti (Gostin, 2023). Non-glandular trichomes of multicellular nature with acute tip were reported in Euphorbia hirta (Pinto et al, 2014), Euphorbia heterophylla, respectively (Essiett et al, 2012), Carthamus tinctoria, Peganum harmala, and Ricinus communis (Dastagir et al, 2023). In spite of taxonomic uses, these trichomes have various physiological and ecological roles in plants.…”