Anatomical basis of a passive mechanism for ovum retention at the ampulloisthmic junction

Halbert, Sheridan A.; Szal, S. E.; Broderson, Stevan H.

doi:10.1002/ar.1092210408

Cited by 12 publications

(2 citation statements)

References 12 publications

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“…Our study evaluated, by single blastocyst profiling, the genetic and epigenetic impacts of modified protocols to produce embryos in vitro that mimic, as far as possible, the physiological conditions of fertilization and early embryo development. This imitation of the natural environment was first approached in both gametes separately: in the male gamete, by using sperm selection procedures that avoided centrifugations, and sperm washing and processing media containing oviductal fluid from the pre-ovulatory phase of the cycle; and, in the female gamete, by preincubating the oocytes within the precise fluid they encounter when, after ovulation, they are transported through the ampulla of the oviduct to the fertilization site, at the ampullar-isthmic junction (Halbert et al, 1988). Secondly, two experimental groups were established for a comparison with the in vivo specimens, where either BSA or reproductive fluids (obtained sequentially at the corresponding phases of the cycle) were added at every step of the IVF and EC procedures.…”

Section: Discussionmentioning

confidence: 99%

DNA methylation and gene expression changes derived from assisted reproductive technologies can be decreased by reproductive fluids

Cánovas

Ivanova

Romar

et al. 2017

eLife

134

139

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The number of children born since the origin of Assisted Reproductive Technologies (ART) exceeds 5 million. The majority seem healthy, but a higher frequency of defects has been reported among ART-conceived infants, suggesting an epigenetic cost. We report the first whole-genome DNA methylation datasets from single pig blastocysts showing differences between in vivo and in vitro produced embryos. Blastocysts were produced in vitro either without (C-IVF) or in the presence of natural reproductive fluids (Natur-IVF). Natur-IVF embryos were of higher quality than C-IVF in terms of cell number and hatching ability. RNA-Seq and DNA methylation analyses showed that Natur-IVF embryos have expression and methylation patterns closer to in vivo blastocysts. Genes involved in reprogramming, imprinting and development were affected by culture, with fewer aberrations in Natur-IVF embryos. Methylation analysis detected methylated changes in C-IVF, but not in Natur-IVF, at genes whose methylation could be critical, such as IGF2R and NNAT.DOI: http://dx.doi.org/10.7554/eLife.23670.001

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Section: Discussionmentioning

confidence: 99%

DNA methylation and gene expression changes derived from assisted reproductive technologies can be decreased by reproductive fluids

Cánovas

Ivanova

Romar

et al. 2017

eLife

134

139

View full text Add to dashboard Cite

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“…3). This may be related to the fact that the egg is held in the isthmus–ampullae junction by 24 h after ovulation (Halbert et al, 1988). The fertilized ovum stays for approximately 36 h in the isthmus (days 2–3 of pregnancy) (Greenwald, 1961; Harper, 1994).…”

Section: Discussionmentioning

confidence: 99%

Histomorphological Changes in the Oviduct Epithelium of the Rabbit During Early Pregnancy

Anzaldúa¹,

Cerbón²

2002

Anat Histol Embryol

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In this study, we determined the histomorphological changes in different regions of the oviduct (fimbriae, infundibulum, ampullae and isthmus) during early pregnancy (days 1-4) of the rabbit. It was observed that the number of secretory cells (PAS-positive cells) diminished in the fimbriae during the first 4 days of pregnancy with the corresponding increase in the number of non-secretory cells (PAS-negative cells). In contrast, there was an increase in the number of PAS-positive cells in the isthmus during early pregnancy. In both the infundibulum and the ampullae the number of PAS-positive cells was increased on day 3. A significant decrease in the height of the epithelium was observed in the fimbriae during early pregnancy. On the contrary, a significant increase in this parameter was observed in the isthmus during the first 3 days of pregnancy. A slight decrease in the height of the epithelium was observed in the infundibulum on day 4 of pregnancy, while in the ampullae it was significantly increased on days 1 and 3. The overall results indicate that during early pregnancy there is a selective modification in the histomorphological characteristics of the different regions of the rabbit oviduct.

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Identification of novel and known ovary‐specific genes including ZP2, in a marsupial, the stripe‐faced dunnart

Whitley²,

Vaux

et al. 2007

Molecular Reproduction Devel

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During the early stages of oogenesis, oocyte-specific factors, synthesized by and stored within the oocyte, play critical roles during oogenesis, folliculogenesis, fertilization and early embryonic development in the mouse. The identification of marsupial maternal factors, expressed specifically in the ovary or oocyte, may provide an insight into the conserved evolutionary mechanisms that drive mammalian oocyte development to cleavage stages. In this study, 10 clones including dunnart ZP2 and c-mos, isolated by cDNA representational difference analysis, were validated by RT-PCR for ovary-specific expression. This novel combination of techniques to isolate ovary-specific genes has identified three novel genes with ovary-specific expression. Both dunnart ZP2 and c-mos exhibited ovary-specific expression, making this study the first isolation of c-mos in a marsupial species. Dunnart ZP2 expression was examined in detail by in situ hybridization and results indicate oocyte-specific expression of dunnart ZP2 in the cytoplasm of oocytes of primordial, primary and secondary follicles with expression being highest in oocytes of primary follicles. ZP2 was not expressed in granulosa cells of any follicles.

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Anatomical basis of a passive mechanism for ovum retention at the ampulloisthmic junction

Cited by 12 publications

References 12 publications

DNA methylation and gene expression changes derived from assisted reproductive technologies can be decreased by reproductive fluids

DNA methylation and gene expression changes derived from assisted reproductive technologies can be decreased by reproductive fluids

Histomorphological Changes in the Oviduct Epithelium of the Rabbit During Early Pregnancy

Identification of novel and known ovary‐specific genes including ZP2, in a marsupial, the stripe‐faced dunnart

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