“…In collaboration with the Visible Heart ® Laboratory and the Cardiothoracic and Vascular Education Center at the University of Minnesota (Minneapolis, MN, USA) and with appropriate consents from LifeSource, a local organ procurement organization (Minneapolis, MN, USA), we perfusion-fixed and gelled 3 explanted hearts in an end-diastolic state immediately following organ recovery. 6 Micro-computed tomography (μCT) images (×3000) were obtained (North Star Imaging, Rogers, MN, USA) with a voxel resolution of 90-120 μm (which are resolutions that cannot be readily obtained during in vivo imaging). 7 The anonymized datasets were imported into the Mimics ® Innovation Suite software (Materialise NV, Leuven, Belgium) for segmenting and generating a 3D computational model of the heart.…”