Anatomy and Comparative Transcriptome Reveal the Mechanism of Male Sterility in Salvia miltiorrhiza

Liao, Jinqiu; Zhang, Zhizhou; Shang, Yukun; Jiang, Yuanyuan; Su, Zhan; Deng, Xuexue; Xiang, Ping; Yang, Ruiwu; Zhang, Li

doi:10.3390/ijms241210259

Cited by 2 publications

(2 citation statements)

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“…The RNA-seq data of anther of S. miltiorrhiza_SC and S. miltiorrhiza_SD at different anther developmental stages published by our team [33] (publicly accessible at NCBI, https://www.ncbi.nlm.nih.gov/, accessed on 28 July 2022) were used to explore the expression patterns and differentially expressed genes of SmMADS genes during meiosis stage (MEI), young microspore stage (YM), and mature pollen stage (MP). Gene expression levels were estimated using fragments per kilobase of transcript per million fragments sequenced (FRKM).…”

Section: Plant Materials Expression Profile Analysis Of Smmads Genesmentioning

confidence: 99%

“…Notably, Sichuan ecotype ( S. miltiorrhiza _SC) is rich in salvianolic acid B, while Shandong ecotype ( S. miltiorrhiza _SD) is rich in tanshinone IIA [ 31 , 32 ]. Furthermore, the Sichuan ecotype exhibits male sterility [ 32 , 33 ]. However, it is still unclear whether MADS-box genes are involved in regulating the male sterility of S. miltiorrhiza .…”

Section: Introductionmentioning

confidence: 99%

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Genome-Wide Analysis of the MADS-box Gene Family and Expression Analysis during Anther Development in Salvia miltiorrhiza

Chai

Deng

et al. 2023

IJMS

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MADS-box genes constitute a large family of transcription factors that play important roles in plant growth and development. However, our understanding of MADS-box genes involved in anther development and male sterility in Salvia miltiorrhiza is still limited. In this study, 63 MADS-box genes were identified from the genome of the male sterility ecotype Sichuan S. miltiorrhiza (S. miltiorrhiza_SC) unevenly distributed among eight chromosomes. Phylogenetic analysis classified them into two types and 17 subfamilies. They contained 1 to 12 exons and 10 conserved motifs. Evolution analysis showed that segmental duplication was the main force for the expansion of the SmMADS gene family, and duplication gene pairs were under purifying selection. Cis-acting elements analysis demonstrated that the promoter of SmMADS genes contain numerous elements associated with plant growth and development, plant hormones, and stress response. RNA-seq showed that the expression levels of B-class and C-class SmMADS genes were highly expressed during anther development, with SmMADS11 likely playing an important role in regulating anther development and male fertility in S. miltiorrhiza_SC. Overall, this study provides a comprehensive analysis of the MADS-box gene family in S. miltiorrhiza, shedding light on their potential role in anther development and male sterility.

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Section: Plant Materials Expression Profile Analysis Of Smmads Genesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Genome-Wide Analysis of the MADS-box Gene Family and Expression Analysis during Anther Development in Salvia miltiorrhiza

Chai

Deng

et al. 2023

IJMS

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Male sterility is related to the imbalance of reactive oxygen species homeostasis in Prunus sibirica

Wang,

Zhang,

Fan

et al. 2024

Physiologia Plantarum

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Prunus sibirica is an economically important forest tree with great development prospects. To study the mechanisms of male sterile P. sibirica, we compared the phenotypic, cytological, and physiological characteristics of male sterile clone 1 with those of male fertile clone 60. Phenotypic characteristics of male sterile P. sibirica included abnormal anther dehiscence, short and unbent filaments, and distorted pollen. Cytological features of abnormal anther development in male sterile P. sibirica emerged following the late‐uninucleate stage and were characterized by delayed tapetum degeneration, delayed and limited secondary thickening of the endothecium, and incomplete mitotic division of pollen. Physiological traits of male sterile P. sibirica included excessive accumulation of reactive oxygen species (ROS) and low antioxidant enzyme activity. At the early‐binucleate stage, the content of malondialdehyde, superoxide radicals, and hydrogen peroxide in male sterile clone 1 was 2.48, 1.27, and 1.34 times of those in male fertile clone 60, respectively. At the late‐binucleate stage, superoxide dismutase in male sterile clone 1 was 50.48% of that in male fertile clone 60, and peroxidase activity in male sterile clone 1 was 36.62% of that in male fertile clone 60. In conclusion, we identified the characteristics and critical period of male sterility in P. sibirica, revealing the association between an imbalance in ROS homeostasis and male sterility.

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Anatomy and Comparative Transcriptome Reveal the Mechanism of Male Sterility in Salvia miltiorrhiza

Cited by 2 publications

References 51 publications

Genome-Wide Analysis of the MADS-box Gene Family and Expression Analysis during Anther Development in Salvia miltiorrhiza

Genome-Wide Analysis of the MADS-box Gene Family and Expression Analysis during Anther Development in Salvia miltiorrhiza

Male sterility is related to the imbalance of reactive oxygen species homeostasis in Prunus sibirica

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