Anatomy, histochemistry, and immunohistochemistry of the olfactory subsystems in mice

Barrios, Arthur W.; Núñez, Gonzalo A.; Sánchez‐Quinteiro, Pablo; Salazar, Ignacio

doi:10.3389/fnana.2014.00063

Cited by 85 publications

(98 citation statements)

References 65 publications

(77 reference statements)

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“…Salazar and Sánchez Quinteiro [2003] as well as Barrios et al [2014] have also reported that UEA-I reacts with receptor cells of the VNE that express vomeronasal receptor 1 in the middle region, but not with those that express vomeronasal receptor 2 in the basal region. Because the rostral and caudal glomeruli of the mouse receive input fibers from vomeronasal receptor cells expressing vomeronasal receptors 1 and 2, respectively, UEA-I has generally been regarded as an indicator of glomeruli that possess different properties.…”

Section: Uea-i Reaction In the Aobmentioning

confidence: 99%

“…Some studies [Lipscomb et al, 2003;Salazar and Sán-chez Quinteiro, 2003;Barrios et al, 2014] have found that UEA-I reacts more intensely with rostral than caudal glomeruli in the AOB. Salazar and Sánchez Quinteiro [2003] as well as Barrios et al [2014] have also reported that UEA-I reacts with receptor cells of the VNE that express vomeronasal receptor 1 in the middle region, but not with those that express vomeronasal receptor 2 in the basal region.…”

Section: Uea-i Reaction In the Aobmentioning

confidence: 99%

“…Although the results did not differ between ICR and C57BL/6J mice in this study, Salazar et al [2001] found that UEA-I does not react with the MOB of adult BALB/c mice, suggesting that strain differences are involved. Because Barrios et al [2014] also described that UEA-I does not react with the OE or with the MOB of BALB/c mice >10 months of age, it may be that age also affects UEA-I histochemistry in the olfactory system. In fact, Tisay et al [2002] described that another glycan structure detected by the lectin, Dolichos biflorus agglutinin, is included in the secondary olfactory pathway of BALB/c, but not C57BL/6, CBA or Quackenbush mice.…”

Section: Uea-i Reaction In the Aobmentioning

confidence: 99%

“…Because α1-2Fuc glycan also mediates neuronal functions and affects neuronal morphology [Pohle et al, 1987;Krug et al, 1991Krug et al, , 1994Matthies et al, 1996;Lorenzini et al, 1997;Kalovidouris et al, 2005;Murrey et al, 2006], α1-2Fuc attachments might affect NCAM function and mediate both major adjustments within the olfactory pathway and minor adjustments within the MOB. The histological localization of α1-2Fuc at various sites within the olfactory system using UEA-I [Lundh et al, 1989;Ducray et al, 1999;Lipscomb et al, 2002Lipscomb et al, , 2003Salazar et al, 2001;Salazar and Sánchez Quinteiro, 2003;Murrey et al, 2009;Barrios et al, 2014;Kondoh et al, 2014] might depend on differences in sex, age, mouse strain, methods of section preparation, UEA-I batch, fixative or the method used to detect positive reactions and/or cutting angles. Furthermore, the time of the day when the mice are sacrificed might affect not only the amount of α1-2Fuc glycan but also its localization in the MOB, although our preliminary prolonged staining with UEA-I-staining showed that UEA-I-binding regions are similar at the layer level during the day and night (data not shown).…”

Section: Uea-i Reaction In the Aobmentioning

confidence: 99%

“…Although α1-2Fuc glycan has been histochemically localized in the mouse olfactory system using Ulex europaeus agglutinin-I (UEA-I) [Lundh et al, 1989;Ducray et al, 1999;Salazar et al, 2001;Lipscomb et al, 2002Lipscomb et al, , 2003Salazar and Sánchez Quinteiro, 2003;Murrey et al, 2009;Barrios et al, 2014;Kondoh et al, 2014], the findings are diverse. For example, we showed that α1-2Fuc glycan preferentially locates in the LOT and in the glomerular layer of the AOB of the ICR mouse [Kondoh et al, 2014] whereas other study groups have not found that UEA-I reacts with the mouse LOT.…”

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confidence: 99%

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Localization of α1-2 Fucose Glycan in the Mouse Olfactory Pathway

Kondoh

Kamikawa²,

Sasaki³

et al. 2016

Cells Tissues Organs

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Glycoconjugates in the olfactory system play critical roles in neuronal formation, and α1-2 fucose (α1-2Fuc) glycan mediates neurite outgrowth and synaptic plasticity. Histochemical findings of α1-2Fuc glycan in the mouse olfactory system detected using Ulex europaeus agglutinin-I (UEA-I) vary. This study histochemically assessed the main olfactory and vomeronasal pathways in male and female ICR and C57BL/6J mice aged 3-4 months using UEA-I. Ulex europaeus agglutinin-I reacted with most receptor cells arranged mainly at the basal region of the olfactory epithelium. The olfactory nerve layer and glomerular layer of the main olfactory bulb were speckled with positive UEA-I staining, and positive fibers were scattered from the glomerular to the internal plexiform layer. The lateral olfactory tract and rostral migratory stream were also positive for UEA-I. We identified superficial short-axon cells, interneurons of the external plexiform layer, external, middle and internal tufted cells, mitral cells and granule cells as the origins of the UEA-I-positive fibers in the main olfactory bulb. The anterior olfactory nucleus, anterior piriform cortex and olfactory tubercle were negative for UEA-I. Most receptor cells in the vomeronasal epithelium and most glomeruli of the accessory olfactory bulb were positive for UEA-I. Our findings indicated that α1-2Fuc glycan is located within the primary and secondary, but not the ternary, pathways of the main olfactory system, in local circuits of the main olfactory bulb and within the primary, but not secondary, pathway of the vomeronasal system.

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Section: Uea-i Reaction In the Aobmentioning

confidence: 99%

Section: Uea-i Reaction In the Aobmentioning

confidence: 99%

Section: Uea-i Reaction In the Aobmentioning

confidence: 99%

Section: Uea-i Reaction In the Aobmentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

Localization of α1-2 Fucose Glycan in the Mouse Olfactory Pathway

Kondoh

Kamikawa²,

Sasaki³

et al. 2016

Cells Tissues Organs

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Revisiting the Vomeronasal System From an Integrated Perspective

Salazar

Barrios

Sánchez‐Quinteiro

2016

The Anatomical Record

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"Olfactory subsystems" is a relatively new terminology to refer to the different regions of the nasal cavity featuring olfactory sensory neurons. In mice, the olfactory chemical cues are detected in four well delimited areas: the main olfactory epithelium, the septal organ, Grüneberg's ganglion, and the sensory epithelium of the vomeronasal organ. Nevertheless, such distribution is by no means exhibited by all mammals. In microsmatic mammals -humans included- the only existing olfactory subsystem is the main olfactory epithelium. This raises the question of whether the lack of certain olfactory structures in those species implies that they are unable to identify certain olfactory signals, or on the contrary, their main olfactory epithelium assumes such role. It would be interesting to determine, in the context of biomedical research, if the sense of smell in humans is fully or partially endowed with the wide range of functions assigned to the vomeronasal system in mice. If it is not, presumptive implications of the lack of such functions should be addressed in human health and well-being. Anat Rec, 299:1488-1491, 2016. © 2016 Wiley Periodicals, Inc.

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Cranial Pair I: The Olfactory Nerve

Crespo

Liberia

Blasco-Ibáñez

et al. 2018

The Anatomical Record

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The olfactory nerve constitutes the first cranial pair. Compared with other cranial nerves, it depicts some atypical features. First, the olfactory nerve does not form a unique bundle. The olfactory axons join other axons and form several small bundles or fascicles: the fila olfactoria. These fascicles leave the nasal cavity, pass through the lamina cribrosa of the ethmoid bone and enter the brain. The whole of these fascicles is what is known as the olfactory nerve. Second, the olfactory sensory neurons, whose axons integrate the olfactory nerve, connect the nasal cavity and the brain without any relay. Third, the olfactory nerve is composed by unmyelinated axons. Fourth, the olfactory nerve contains neither Schwann cells nor oligodendrocytes wrapping its axons. But it contains olfactory ensheathing glia, which is a type of glia unique to this nerve. Fifth, the olfactory axons participate in the circuitry of certain spherical structures of neuropil that are unique in the brain: the olfactory glomeruli. Sixth, the axons of the olfactory nerve are continuously replaced and their connections in the central nervous system are remodeled continuously. Therefore, the olfactory nerve is subject to lifelong plasticity. Finally seventh, the olfactory nerve can be a gateway for the direct entrance of viruses, neurotoxins and other xenobiotics to the brain. In the same way, it can be used as a portal of entry to the brain for therapeutic substances, bypassing the blood-brain barrier. In this article, we analyze some features of the anatomy and physiology of the first cranial pair. Anat Rec, 2018. © 2018 Wiley Periodicals, Inc.

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Anatomy, histochemistry, and immunohistochemistry of the olfactory subsystems in mice

Cited by 85 publications

References 65 publications

Localization of α1-2 Fucose Glycan in the Mouse Olfactory Pathway

Localization of α1-2 Fucose Glycan in the Mouse Olfactory Pathway

Revisiting the Vomeronasal System From an Integrated Perspective

Cranial Pair I: The Olfactory Nerve

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