Ancestral and offspring nutrition interact to affect life history traits in<i>Drosophila melanogaster</i>

Deas, Joseph B.; Blondel, Leo; Extavour, Cassandra G.

doi:10.1101/488809

Cited by 7 publications

(12 citation statements)

References 39 publications

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“…Since high protein diet did not confer any change in the pupal size of the flies, but high carbohydrate diet resulted in smaller pupa (Reis, 2016), it is surprising to see pupal size difference upon protein restriction. This is in line with the results of Deas et al, 2019; that suggests more susceptibility of pupal mass change in poor diet than that of rich nutrient diet, in addition to exhibiting effects of parental and grandparental diet (Deas et al, 2019). Overall, our results also show that diet and generation have a differential role of different traits as suggested elsewhere (Deas et al, 2019).…”

Section: Discussionsupporting

confidence: 89%

“…This is in line with the results of Deas et al, 2019; that suggests more susceptibility of pupal mass change in poor diet than that of rich nutrient diet, in addition to exhibiting effects of parental and grandparental diet (Deas et al, 2019). Overall, our results also show that diet and generation have a differential role of different traits as suggested elsewhere (Deas et al, 2019).…”

Section: Discussionsupporting

confidence: 89%

“…Overall, our results also show that diet and generation have a differential role of different traits as suggested elsewhere (Deas et al, 2019).…”

Section: Body Size and Pupal Size:-supporting

confidence: 88%

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Trans-generational effect of protein restricted diet on adult body and wing size ofDrosophila melanogaster

Krittika

Yadav

2020

Preprint

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24Dietary Restriction (DR) via protein restriction (PR) has turned out to be a very inquisitive field 25 as past studies opened about the feasible trade-offs between various fitness and behavioral traits 26 in Drosophila melanogaster to understand lifespan or aging in a nutritionally challenged 27 environment. However, the phenotypes of body size, weight and wing length respond according 28 to the factors such as flies' genotype, environmental exposure and parental diet. Hence, 29 understanding the long-term effect of PR on these phenotypes is essential. Here, we demonstrate 30 the effect of PR diet on body size, weight and normal & dry wing length of flies subjected to 31 PR50 & PR70 (50% & 70% protein content present in control food respectively) for 20 32 generations from the pre-adult stage. We found that the PR fed flies have lower body weight, 33 relative water content (in males), smaller normal & dry body size as compared to its control and 34 generations 1 & 2. Interestingly, the wing size of PR flies and the pupal size of PR70 flies are 35 smaller and also showed significant effects of diet and generation. Thus, these traits are sex and 36 generation dependent along with an interaction of diet, which is capable of modulating these 37 results variably. Our study suggests that the trans-generational effect is more prominent in 38 influencing these traits and moreover wing length might not be a predictor for body size. Taken 39 together, the trans-generational effect of dietary protein restriction on fitness and fitness-related 40 traits might be helpful to understand the underpinning mechanisms pertaining to evolution and 41 aging in fruit flies D. melanogaster. 42 43 44 45 46 47 48 49 50 3 Introduction: 51 Organisms vary in body size not only across species, but also within a particular species. The 52 variations in the body composition can influence phenotypic traits like body size, body weight 53 etc., while these trait variations can be attributed to various environmental and genetic factors 1-54 3]. The environmental factors that can influence organismal body size and weight, including 55 wing length (especially in insects) can be nutrition [4], temperature [5-6], crowding [4, 7], 56 latitudinal clines [8] and certain cases of laboratory selection pressures for faster development 57 [9] etc. Body size, weight and wing length are certain parameters that ensure the overall fitness 58 of organisms including fruit flies. Thus, variations in these phenotypes can be used to understand 59 the genotypic changes that are bound to occur [10].60 Fruit flies Drosophila melanogaster for the past three decades, has been widely used as a model 61 organism for studying aging via nutritional approaches including diet restriction (DR), food 62 dilution, intermittent feeding, etc., [11-13]. The diet of fruit flies commonly comprises of 63 carbohydrates and proteins as the major source, with lipids, vitamins, minerals present in minor 64 quantities. Restricting protein source (yeast) in the fly food is a t...

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Section: Discussionsupporting

confidence: 89%

Section: Discussionsupporting

confidence: 89%

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Trans-generational effect of protein restricted diet on adult body and wing size ofDrosophila melanogaster

Krittika

Yadav

2020

Preprint

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“…DNA methylation, histone acetylation) (Xia & de Belle, 2016). The phrase "transgenerational epigenetic inheritance" was used by Deas and colleagues to mark heritable changes in gene expression that occur without a change in DNA sequence (Deas et al 2019).…”

Section: Introductionmentioning

confidence: 99%

Parental dietary sucrose affects metabolic and antioxidant enzyme activities in Drosophila

Strilbytska

Strutynska

Semaniuk

et al. 2021

Entomological Science

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The influence of parental nutrition as a key environmental factor that can influence offspring performance has become a hot topic for modern investigations. In the current study using fruit flies, Drosophila melanogaster, we found the sucrose concentration of the diet in the parental generation had a significant influence on the metabolism and antioxidant defense of the next generation, even when that subsequent generation had been shifted to a standard diet. We found that low concentrations of dietary carbohydrate in the parental generation led to higher activities of lactate dehydrogenase, malate dehydrogenase (MDH) and alanine transaminase (ALT) enzymes and increased urea content in their progeny. Moreover, higher activities of the firstand second-line antioxidant defense enzymes were detected in F1 males generated from parents fed a low-carbohydrate diet. Low sucrose concentration in the parental diet resulted in increased levels of protein thiols but decreased the low-molecular-weight thiol group content in F1 males. The influence of different dietary sucrose concentrations also resulted in sex-dependent differences, where MDH, ALT, glutathione-S-transferase, glucose-6-phosphate dehydrogenase and isocitrate dehydrogenase activities and levels of thiol groups were unaffected in F1 females. The results highlight the transgenerational influence that specific dietary macronutrients provided in the parental diet, namely carbohydrates, can have on the next generation, influencing enzyme activities, metabolic pathways and antioxidant defenses in progeny Drosophila.

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“…However, the advantages that it offers for understanding mechanisms of inheritance have not been commensurately leveraged to explore the inheritance of acquired traits. A few notable exceptions include early studies showing that transcriptional profiles induced by exposing flies to a drug could be observed again in progeny that was not exposed to the same drug (Sharma & Singh, 2009) and numerous studies showing that diet quality in ancestral generations can alter diverse life‐history traits in later generations (such as egg and pupa size, wing length, longevity, fecundity, and predisposition to obesity and metabolic disorders in response to high caloric diets) (Buescher et al, 2013; Deas et al, 2019; Dew‐Budd et al, 2016; Xia & de Belle, 2016). However, studies that specifically address the inheritance of drug tolerance in Drosophila are noticeably lacking.…”

Section: Introductionmentioning

confidence: 99%

Repeated ethanol intoxications of Drosophila melanogaster adults increases the resistance to ethanol of their progeny

Bonilla

McPherson

Coreas

et al. 2021

Alcoholism Clin & Exp Res

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Background: For decades, Drosophila melanogaster has been used as a model organism to understand the genetics and neurobiology of ethanol intoxication and tolerance. Previous research has shown that acute and chronic pre-exposures to ethanol can trigger the development of functional ethanol tolerance in flies and has unveiled some of the genetic pathways involved in the process. To our knowledge, however, no previous work has systematically explored whether repeated intoxications of adult flies can affect the ethanol tolerance of their progeny.Methods: Adult flies were intoxicated several times (once daily, over several days), and their F1 and F2 progeny were subjected to a functional tolerance test in which flies are exposed to ethanol and video recorded twice within 5 hr. Their behavior was subsequently analyzed to determine how long it took them to become sedated during the first and second exposures. One-and 2-way ANOVAs were used to determine whether parental treatment had an effect on their progeny's baseline resistance and/ or acquired functional tolerance to ethanol.Results: Parental flies that were intoxicated several times produced F1 and F2 progeny with a significantly higher resistance to ethanol than progeny from unexposed controls. Further, parental intoxications inconsistently increased the progeny's capacity to develop rapid functional tolerance upon re-exposure to ethanol. The transmission of increased ethanol resistance to progeny lasted several days after the last parental intoxication. Conclusion:To our knowledge, this is the first demonstration that repeated parental daily intoxications affect the progeny's response to ethanol in fruit flies. Our findings support the use of D. melanogaster to explore conserved pathways underlying the transmission of ethanol tolerance and can help in the identificaton of novel strategies for managing alcohol use disorder.

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Ancestral and offspring nutrition interact to affect life history traits inDrosophila melanogaster

Cited by 7 publications

References 39 publications

Trans-generational effect of protein restricted diet on adult body and wing size ofDrosophila melanogaster

Trans-generational effect of protein restricted diet on adult body and wing size ofDrosophila melanogaster

Parental dietary sucrose affects metabolic and antioxidant enzyme activities in Drosophila

Repeated ethanol intoxications of Drosophila melanogaster adults increases the resistance to ethanol of their progeny

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