AnCF, the CCAAT Binding Complex of <i>Aspergillus nidulans</i>, Is Essential for the Formation of a DNase I-Hypersensitive Site in the 5′ Region of the <i>amdS</i> Gene

Narendja, Frank; Davis, Meryl A.; Hynes, Michael J.

doi:10.1128/mcb.19.10.6523

Cited by 43 publications

(31 citation statements)

References 77 publications

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“…AnCF acts at CCAAT sequences to affect the expression of a diverse array of genes with no obvious common role in metabolism (7). Binding of AnCF to a CCAAT sequence in the 5Ј region of the amdS gene has been shown to result in the formation of a nucleosome-free region (32). Here, we have shown that AcuFLacZ expression is greatly reduced in a strain with a deletion in the gene encoding the HapC subunit of AnCF.…”

Section: Vol 184 2002 Phosphoenolpyruvate Carboxykinase Of a Nidulansmentioning

confidence: 65%

Regulation of theacuFGene, Encoding Phosphoenolpyruvate Carboxykinase in the Filamentous FungusAspergillus nidulans

2002

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Phosphoenolpyruvate carboxykinase (PEPCK) is a key enzyme required for gluconeogenesis when microorganisms grow on carbon sources metabolized via the tricarboxylic acid (TCA) cycle. Aspergillus nidulans acuF mutants isolated by their inability to use acetate as a carbon source specifically lack PEPCK. The acuF gene has been cloned and shown to encode a protein with high similarity to PEPCK from bacteria, plants, and fungi. The regulation of acuF expression has been studied by Northern blotting and by the construction of lacZ fusion reporters. Induction by acetate is abolished in mutants unable to metabolize acetate via the TCA cycle, and induction by amino acids metabolized via 2-oxoglutarate is lost in mutants unable to form 2-oxoglutarate. Induction by acetate and proline is not additive, consistent with a single mechanism of induction. Malate and succinate result in induction, and it is proposed that PEPCK is controlled by a novel mechanism of induction by a TCA cycle intermediate or derivative, thereby allowing gluconeogenesis to occur during growth on any carbon source metabolized via the TCA cycle. It has been shown that the facB gene, which mediates acetate induction of enzymes specifically required for acetate utilization, is not directly involved in PEPCK induction. This is in contrast to Saccharomyces cerevisiae, where Cat8p and Sip4p, homologs of FacB, regulate PEPCK as well as the expression of other genes necessary for growth on nonfermentable carbon sources in response to the carbon source present. This difference in the control of gluconeogenesis reflects the ability of A. nidulans and other filamentous fungi to use a wide variety of carbon sources in comparison with S. cerevisiae. The acuF gene was also found to be subject to activation by the CCAAT binding protein AnCF, a protein homologous to the S. cerevisiae Hap complex and the mammalian NFY complex.

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Section: Vol 184 2002 Phosphoenolpyruvate Carboxykinase Of a Nidulansmentioning

confidence: 65%

Regulation of theacuFGene, Encoding Phosphoenolpyruvate Carboxykinase in the Filamentous FungusAspergillus nidulans

2002

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“…A similar mechanism has been shown for the regulation of the alcA gene of A. niger, where the transcriptional activator AlcR competes with CreA for the corresponding, partially overlapping binding sites (e.g. Marmorstein et al 1992;Marmorstein and Harrison 1994;Mathieu and Felenbok 1994;Narendja et al 1999). …”

Section: Interplay Of Xyr1 With Specific Transcriptional Regulators Amentioning

confidence: 95%

“…The Hap2/3/5 complex contacts the CCAAT box within this motif. The CCAAT box, which is present in many fungal promoters (Brakhage et al 1999), was shown to be involved in the maintenance of an active chromatin structure (Narendja et al 1999). On the other hand, CCAAT boxes in filamentous fungi showing repressing functions (e.g.…”

Section: Interplay Of Xyr1 With Specific Transcriptional Regulators Amentioning

confidence: 99%

Regulation of transcription of cellulases- and hemicellulases-encoding genes in Aspergillus niger and Hypocrea jecorina (Trichoderma reesei)

Stricker

Mach

Graaff

2008

Appl Microbiol Biotechnol

249

182

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“…The Hap2/3/5-binding CCAAT motif in XAE is a common cis-acting element found in the promoter and enhancer regions of a large number of eukaryotic genes (20), including filamentous fungi (3), and has been shown to be involved in maintaining a transcriptionally active chromatin structure (24). We have recently shown that the CCAAT-box within the CAE of H. jecorina cbh2 is involved in nucleosome assembly (S. Zeilinger, M. Leonhartsberger, M. Pail, R. L. Mach, and C. P. Kubicek, unpublished data) and that a mutation of CCAAT to CCCTT within CAE results in a 30% decrease in gene transcription (43).…”

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confidence: 99%

Transcriptional Regulation of xyn2 in Hypocrea jecorina

Würleitner¹,

Pera²,

Wacenovsky³

et al. 2003

Eukaryot Cell

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The xylanase system of the filamentous fungus Hypocrea jecorina (Trichoderma reesei) consists of two specific xylanases, Xyn1 and Xyn2, which are simultaneously expressed during growth on xylan but respond differentially to low-molecular-weight inducers. Using in vivo footprinting analysis of xylan-induced and noninduced mycelia, we detected two adjacent nucleotide sequences (5-AGAA-3 on the noncoding strand and 5-GGGT AAATTGG-3, referred to as the xylanase-activating element [XAE], on the coding strand, respectively) to bind proteins. Among these, binding to the AGAA-box is only observed under noninduced conditions, whereas binding to XAE is constitutive. Electrophoretic mobility shift assay with heterologously expressed components of the H. jecorina Hap2/3/5 protein complex and the cellulase regulator Ace2 suggests that these two transactivators form the protein complex binding to XAE. H. jecorina transformants, containing correspondingly mutated versions of the xyn2 promoter fused to the Aspergillus niger goxA gene as a reporter, revealed that the elimination of protein binding to the AGAA-box resulted in a threefold increase in both basal and induced transcription, whereas elimination of Ace2 binding to its target in XAE completely eliminated transcription under both conditions. Destruction of the CCAAT-box by insertion of a point mutation prevents binding of the Hap2/3/5 complex in vitro and results in a slight increase in both basal and induced transcription. These data support a model of xyn2 regulation based on the interplay of Hap2/3/5, Ace2 and the AGAA-box binding repressor.

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AnCF, the CCAAT Binding Complex of Aspergillus nidulans, Is Essential for the Formation of a DNase I-Hypersensitive Site in the 5′ Region of the amdS Gene

Cited by 43 publications

References 77 publications

Regulation of theacuFGene, Encoding Phosphoenolpyruvate Carboxykinase in the Filamentous FungusAspergillus nidulans

Regulation of theacuFGene, Encoding Phosphoenolpyruvate Carboxykinase in the Filamentous FungusAspergillus nidulans

Regulation of transcription of cellulases- and hemicellulases-encoding genes in Aspergillus niger and Hypocrea jecorina (Trichoderma reesei)

Transcriptional Regulation of xyn2 in Hypocrea jecorina

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