Anchoring of bovine chromosomes 4, 6, 7, 10, and 14 linkage group telomeric ends via FISH analysis of lambda clones

Smith, Timothy P. L.; Lopez-Corrales, Nestor L.; Grosz, M. D.; Beattie, Craig W.; Kappes, S. M.

doi:10.1007/s003359900434

Cited by 25 publications

(6 citation statements)

References 26 publications

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“…Recombination rates between markers bordering the centromere appear reduced in comparison to the more distal regions of BTX. This observation is supported by the assignment of BL1098 to Xpl4 (Smith et al 1996b). The linkage distance between BL1098 and XBM7 is 3.5 cM, yet physical distance between these markers is approximately equivalent to a fourth of the p-arm (Figs.…”

Section: ) Marker Order Was Identical To That Reported By Yeh Et Almentioning

confidence: 58%

“…Methods used to obtain ms markers from M13 bacteriophage libraries (BM and BMS series) and genomic lambda clones (BL series), have been described previously Stone et al 1995;Smith et al 1996b). Preparation of the BTXp mierodissected library and ms identification from the BTXp library were as described (Ponce de Le6n et al 1996), with some modification.…”

Section: Methodsmentioning

confidence: 99%

“…BTXp lambda clones were screened with a (GT)xl radiolabeled probe at high stringency conditions (65~ as described , and sequenced according to the manufacturer's protocol (Sequenase, U.S. Biochemicals, Cleveland, Ohio). All sequenced clones were searched for similarity against: I) repetitive sequences (Alexander et al 1995;Stone et al 1995); 2) ms identified in our laboratory Stone et al 1995;Ponce de Le6n et al 1996;Smith et al 1996b); and 3) ms recorded in GenBank (Benson et al 1993) with software from the Genetics Computer Group, Madison, Wisconsin (GCG, 1994). Primer pairs were designed only from unique clones containing (GT) > 11.…”

Section: Methodsmentioning

confidence: 99%

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An integrated genetic and physical map of the bovine X Chromosome

Sonstegard¹,

Lopez-Corrales²,

Kappes³

et al. 1997

Mammalian Genome

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Abstract. Genotypic data for 56 microsatellites (ms) generated from maternal full sib families nested within paternal half sib pedigrees were used to construct a linkage map of the bovine X Chromosome (Chr) (BTX) that spans 150 cM (ave. interval 2.7 cM). The linkage map contains 36 previously unlinked ms; seven generated from a BTXp library. Genotypic data from these 36 ms was merged into an existing linkage map to more than double the number of informative BTX markers. A male specific linkage map of the pseudoautosomal region was also constructed from five ms at the distal end of BTXq. Four informative probes physically assigned by fluorescence in situ hybridization defined the extent of coverage, confirmed the position of the pseudoautosomal region on the q-arm, and identified a 4.1-cM marker interval containing the centromere of BTX.

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Section: ) Marker Order Was Identical To That Reported By Yeh Et Almentioning

confidence: 58%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

An integrated genetic and physical map of the bovine X Chromosome

Sonstegard¹,

Lopez-Corrales²,

Kappes³

et al. 1997

Mammalian Genome

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show abstract

“…Several strategies exist for generating additional markers for specific regions of the genome containing QTL. These include rnicrodissected, rnicrocloned libraries (Ponce de Le6n et al 1996;Sonstegard et al 1997a,b) and identification of YAC clones (Smith et al 1997) containing the closest flanking markers to the QTL. Additional microsatellites can be identified within a YAC clone (Lench et al 1996) for haplotyping to increase the polymorphic content of a marker locus to the extent that it is informative in almost every animal.…”

Section: Discussionmentioning

confidence: 99%

“…Mezzelani et al (1995) Mezzelani et al (1995 Mezzelani et al (1995 Solinas-Toldo et al (1995) Mezzelani et al (1995) Smith et al (1997 Hawkins et al (1995) Solinas-Toldo et al (1993) Solinas-Toldo et al ) Threadgill and Womack (1990 (1995) Mezzelani et al (1995 Solinas-Toldo et al (1993) Mezzelani et al (1995) Mezzelani et al (1995) Mezzelani et al (1995) …”

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confidence: 99%

A second-generation linkage map of the bovine genome.

Kappes¹,

Keele²,

Stone³

et al. 1997

Genome Res.

467

333

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We report a bovine linkage map constructed with 1236 polymorphic DNA markers and 14 erythrocyte antigens and serum proteins. The 2990-cM map consists of a sex-specific, X chromosome linkage group and 29 sex-averaged, autosomal linkage groups with an average interval size of 2.5 cM. The map contains 627 new markers and 623 previously linked markers, providing a basis for integrating the four published bovine maps. Orientation and chromosomal assignment of all the linkage groups, except BTA20 and BTA22, was provided by 88 markers that were assigned previously to chromosomes. This map provides sufficient marker density for genomic scans of populations segregating quantitative trait loci (QTL) and subsequent implementation of marker-assisted selection (MAS) mating schemes.

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A radiation hybrid map for the bovine Y Chromosome

et al. 2002

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Screening a bovine Y Chromosome-specific DNA library resulted in 34 new microsatellites, six of which mapped to the pseudoautosomal region (PAR), and 28 localized to the Y-specific region. These microsatellites, together with 23 markers previously mapped to the bovine Y Chr, were scored on a 7000-rad cattle-hamster radiation hybrid (RH) panel. Retention frequency of individual markers ranged from 18.5% to 76.5% with an average of 48.4%. Markers with high retention frequency (>55%) were found to exist in multiple copies on the Y Chr. Thirteen markers were placed on the PAR RH map with the AmelY gene proximal to the pseudoautosomal boundary and 46 markers, including Sry and Tspy gene, on the Y-specific region of the RH map. The microsatellites developed and mapped in this work will be useful for comparative mapping of cattle, sheep, and goat, studying the origin, evolution, and migration of bovidae species and provide an initial platform to develop a high-resolution map of the Y Chr and positional cloning of Y-specific genes.

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Anchoring of bovine chromosomes 4, 6, 7, 10, and 14 linkage group telomeric ends via FISH analysis of lambda clones

Cited by 25 publications

References 26 publications

An integrated genetic and physical map of the bovine X Chromosome

An integrated genetic and physical map of the bovine X Chromosome

A second-generation linkage map of the bovine genome.

A radiation hybrid map for the bovine Y Chromosome

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