Ancient DNA provides new insights into the origin of the Chinese domestic horse

Tang, Zhuowei; Han, Lu; Speller, Camilla; Yang, Dongya; Ma, Xiaolin; Cao, Jianen; Zhu, Hong; Zhou, Hui

doi:10.1016/j.jas.2008.11.006

Cited by 42 publications

(33 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Maternal genetic diversity of the Arabian populations described in this study was similar to that reported in some other breeds [17,33]. Although Syrian, Shagya Arabian and Iranian Arabian populations had equally high HapD values (Table 1), the Syrian population was the most variable based on the consensus Neighbor-joining tree (Figure 1) where the Syrian individuals were found in eight clades compared to the Iranian Arabian and Shagya Arabian individuals found only in five and three clades, respectively.…”

Section: Discussionsupporting

confidence: 89%

Maternal phylogenetic relationships and genetic variation among Arabian horse populations using whole mitochondrial DNA D-loop sequencing

Khanshour

Cothran

2013

BMC Genet

View full text Add to dashboard Cite

BackgroundMaternal inheritance is an essential point in Arabian horse population genetics and strains classification. The mitochondrial DNA (mtDNA) sequencing is a highly informative tool to investigate maternal lineages. We sequenced the whole mtDNA D-loop of 251 Arabian horses to study the genetic diversity and phylogenetic relationships of Arabian populations and to examine the traditional strain classification system that depends on maternal family lines using native Arabian horses from the Middle East.ResultsThe variability in the upstream region of the D-loop revealed additional differences among the haplotypes that had identical sequences in the hypervariable region 1 (HVR1). While the American-Arabians showed relatively low diversity, the Syrian population was the most variable and contained a very rare and old haplogroup. The Middle Eastern horses had major genetic contributions to the Western horses and there was no clear pattern of differentiation among all tested populations. Our results also showed that several individuals from different strains shared a single haplotype, and individuals from a single strain were represented in clearly separated haplogroups.ConclusionsThe whole mtDNA D-loop sequence was more powerful for analysis of the maternal genetic diversity in the Arabian horses than using just the HVR1. Native populations from the Middle East, such as Syrians, could be suggested as a hot spot of genetic diversity and may help in understanding the evolution history of the Arabian horse breed. Most importantly, there was no evidence that the Arabian horse breed has clear subdivisions depending on the traditional maternal based strain classification system.

show abstract

Section: Discussionsupporting

confidence: 89%

Maternal phylogenetic relationships and genetic variation among Arabian horse populations using whole mitochondrial DNA D-loop sequencing

Khanshour

Cothran

2013

BMC Genet

View full text Add to dashboard Cite

show abstract

“…It has been proposed therefore that Przewalski horses are not ancestral to modern domestic horses 23,30 . However, the Przewalski haplotypes do fall within the large diversity of modern horse mtDNA 14,15,17,25 .…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, DNA amplified from ancient remains provides the only means to investigate the extent and nature of the genetic diversity of wild horses. Thus far, this approach has been used only for mtDNA 13,14,16,[23][24][25] the results of which suggest that domestication was not a significant bottleneck for horse mtDNA diversity 26 . Consequently, high mtDNA diversity in domestic horses has been explained by high diversity of the founding population, multiple origins of domestication, further domestication events during the Iron Age, and backcrossing with wild mares from different populations.…”

mentioning

confidence: 99%

Discovery of lost diversity of paternal horse lineages using ancient DNA

et al. 2011

View full text Add to dashboard Cite

modern domestic horses display abundant genetic diversity within female-inherited mitochondrial DnA, but practically no sequence diversity on the male-inherited Y chromosome. several hypotheses have been proposed to explain this discrepancy, but can only be tested through knowledge of the diversity in both the ancestral (pre-domestication) maternal and paternal lineages. As wild horses are practically extinct, ancient DnA studies offer the only means to assess this ancestral diversity. Here we show considerable ancestral diversity in ancient male horses by sequencing 4 kb of Y chromosomal DnA from eight ancient wild horses and one 2,800-year-old domesticated horse. Both ancient and modern domestic horses form a separate branch from the ancient wild horses, with the Przewalski horse at its base. our methodology establishes the feasibility of re-sequencing long ancient nuclear DnA fragments and demonstrates the power of ancient Y chromosome DnA sequence data to provide insights into the evolutionary history of populations.

show abstract

“…Haplotypes A, K3, and X3 were the most common in the Hucul horse identified by Cieslak et al (2010). Cai et al (2009) indicated a dominance of the A haplogroup in most modern horses. Haplogroup A2 has not been found in any breed other than the Przewalski horse (Jansen et al, 2002) and was also detected in our study in Przewalski Wild horse samples from Hortobágy and Köln.…”

Section: Mtdna Analysismentioning

confidence: 94%

Genetic characterization and population bottleneck in the Hucul horse based on microsatellite and mitochondrial data

Kusza

Priskin

Ivanković

et al. 2013

Biol J Linn Soc Lond

View full text Add to dashboard Cite

The aim of this work was to gather information about the origin and genetic characterization of the Central European Hucul horse based on 71 horses using 17 microsatellites and the D‐loop region of mtDNA. Their genetic relationship to the Polish Konik (N = 7), German (N = 4) and Hungarian wild Przewalski horses (N = 4) and 200 horse sequences from GenBank was also analysed. Both microsatellite and mtDNA analysis showed a high genetic variation in the Hucul. A total of 130 alleles were detected, the mean number of observed alleles per microsatellite was 7.647, and the number of effective alleles was 4.401. The average observed and expected heterozygosity were 0.706 and 0.747, respectively. The high heterozygosity values and Wright's fixation index (FIS) (−0.128) indicated a low level of inbreeding, low or no selection pressure, and large number of alleles. mtDNA analysis revealed 18 haplotypes for the Hucul population with a total of 23 variable sites. Haplotype and nucleotide diversities were 0.935 ± 0.011 and 0.022 ± 0.012, respectively. Neutrality tests (Tajima's D and Fu's Fs) were non‐significant, and mismatch distribution was ragged, indicating that the Hucul population is in genetic equilibrium. The most frequent mtDNA D‐loop region belonged to haplogroup A (48%), which was also present in Przewalski Wild horse samples, while Polish Konik samples belonged to three haplotypes and C, F, and G haplogroups. Large and significant pairwise ΦST values along with a small number of common haplotypes indicated a low level of gene flow and lack of genetic structure among the three studied breeds (Hucul, Konik, and Przewalski Wild horse). The present work contributes to our knowledge of the genetic diversity of the Hucul horse and helps to define its genetic conservation. © 2013 The Linnean Society of London, Biological Journal of the Linnean Society, 2013, 109, 54–65.

show abstract

Ancient DNA provides new insights into the origin of the Chinese domestic horse

Cited by 42 publications

References 23 publications

Maternal phylogenetic relationships and genetic variation among Arabian horse populations using whole mitochondrial DNA D-loop sequencing

Maternal phylogenetic relationships and genetic variation among Arabian horse populations using whole mitochondrial DNA D-loop sequencing

Discovery of lost diversity of paternal horse lineages using ancient DNA

Genetic characterization and population bottleneck in the Hucul horse based on microsatellite and mitochondrial data

Contact Info

Product

Resources

About