Ancient introgression of Lepus timidus mtDNA into L. granatensis and L. europaeus in the Iberian Peninsula

Alves, Paulo C.; Ferrand, Nuno; Suchentrunk, Franz; Harris, D. James

doi:10.1016/s1055-7903(02)00417-7

Cited by 118 publications

(147 citation statements)

References 31 publications

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“…Hence, the arctic and temperate fauna must have repeatedly replaced each other in large geographical regions, with the possibilities of transient contacts and hybridization between closely related arctic and temperate species. We have found evidence for this phenomenon in the Iberian Peninsula where the three extant temperate hare species harbour high frequencies of mtDNA from an arctic species, Lepus timidus (Alves et al 2003;Melo-Ferreira et al 2005. Here we extend our previous geographical survey of this phenomenon in Iberia, and also review the available molecular data on other hare species.…”

Section: Introductionmentioning

confidence: 51%

“…This would imply that L. castroviejoi is closely related to L. timidus. However, this is contradicted by nuclear gene phylogenies showing that L. timidus is roughly equidistantly related to L. castroviejoi, L. granatensis and L. europaeus (Alves et al 2003. Therefore, we suggest that the true original L. castroviejoi mtDNA has not been sampled, but that instead the two lineages of mtDNA found in this species are of L. timidus origin and witness two introgression events of different ages.…”

Section: Circumpolar Mitochondrial Dna Introgression?mentioning

confidence: 60%

“…Each of these species is characterized by a mitochondrial DNA lineage of its own, but in Iberia all three species also share a fourth lineage, hereafter labelled 'foreign', the representatives of which are more related to each other than they are to any of the three species-specific lineages (Alves et al 2003;Melo-Ferreira et al 2005). The average divergence of the foreign lineage to L. granatensis and L. europaeus is approximately 10%, a typical value for interspecific differences in mammals (Johns & Avise 1998), while the divergence to L. castroviejoi is approximately 3% (Alves et al 2003). This foreign lineage is clearly related to mtDNA of L. timidus, which suggests that its presence in the Iberian species resulted from secondary : 33 from L. granatensis (Ala1, Á lava; Alb, Albacete; Alc, Alcañ iz; Ali, Alicante; Alj, Aljustrel; Amd, Almeida; Ben, Benavente; Brg, Bragança; CBr, Castelo Branco; Cac, Cáceres; Crd, Có rdoba; Crn, La Coruñ a; Cre, Ciudad Real; Cue, Cuenca; Grn, Granada; Hue, Huesca; Mad, Madrid; Mer, Mértola; Mur, Mú rcia; Nav1, Navarra; Pan, Pancas; Ptm, Portimão; Sal, Salamanca; SE, Serra da Estrela; San, Santarém; Sen, Sendim; Sev, Sevilla; Sor, Soria; TC, Tierra de Campos; Tol, Toledo; Tor, Tordesillas; Val, Valência; Zar, Zaragoza); 5 from L. europaeus (Ala2, Á lava; Can, Cantabria; Jac, Jaca; Nav2, Navarra; Vlc, Villarcayo) and 1 from L. castroviejoi (Cas, Cantabria).…”

Section: Massive Mitochondrial Dna Introgression From L Timidus Intomentioning

confidence: 99%

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The ubiquitous mountain hare mitochondria: multiple introgressive hybridization in hares, genusLepus

Alves

Melo‐Ferreira

Freitas

et al. 2008

Phil. Trans. R. Soc. B

120

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Climatic oscillations during the glaciations forced dramatic changes in species distributions, such that some presently temperate regions were alternately occupied by temperate and arctic species. These species could have met and hybridized during climatic transitions. This phenomenon happened for three hare species present in Iberia (Lepus granatensis, Lepus europaeus and Lepus castroviejoi ), which display high frequencies of mitochondrial DNA (mtDNA) from Lepus timidus, an arctic/boreal species presently extinct in Iberia. Here, we extend our previous geographical survey to determine whether the distribution of this mtDNA lineage extends beyond the northern half of the Iberian Peninsula, where it is found at high frequencies. We also review the taxonomy, distribution and molecular phylogeny of the genus Lepus. The phylogenetic inference reveals the presence of L. timidus-like mtDNA in several other hare species in Asia and North America, suggesting that the mitochondrial introgression observed in Iberia might be generalized. Comparison with the available nuclear gene phylogenies suggests that introgression could have happened repeatedly, possibly during different climatic transitions. We discuss demographic and adaptive scenarios that could account for the repetition in time and space of this spectacular phenomenon and suggest ways to improve our understanding of its determinants and consequences. Such high levels of introgressive hybridization should discourage attempts to revise hare taxonomy based solely on mtDNA.

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Section: Introductionmentioning

confidence: 51%

Section: Circumpolar Mitochondrial Dna Introgression?mentioning

confidence: 60%

Section: Massive Mitochondrial Dna Introgression From L Timidus Intomentioning

confidence: 99%

See 1 more Smart Citation

The ubiquitous mountain hare mitochondria: multiple introgressive hybridization in hares, genusLepus

Alves

Melo‐Ferreira

Freitas

et al. 2008

Phil. Trans. R. Soc. B

120

View full text Add to dashboard Cite

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“…In addition, the band patternobtained using HaeIII and HinfI shows differences in the 359 bpcytb fragmentevenamongclosely related species of hares asL. granatensisandL.timidus, which appear in Spain as an introgression of the cytb gene (Alves et al 2003, Melo-Ferreira et al 2012 (Fig. 2A and 3A).…”

Section: Resultsand Discussionmentioning

confidence: 98%

Identification of blood meals in field captured sand flies by a PCR-RFLP approach based on cytochrome b gene

et al. 2015

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Identification of blood meals in field captured sand flies by a PCR-RFLP approach based on cytochrome b gene.Acta Tropica http://dx.doi.org/10. 1016/j.actatropica.2015.08.020 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Therefore, thisapproachis anefficient and reliable alternative method to be applied in entomological surveys.

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“…The Iberian Peninsula has been an area of recurrent admixture at different timescales among species of hares (genus Lepus), because the three extant species have been heavily affected by mtDNA introgression from a fourth species, the mountain hare, Lepus timidus (Alves et al, 2003;Melo-Ferreira et al, 2005), which was present in Iberia at the end of the last glacial period (Altuna, 1970) but is currently distributed only in Northern Eurasia and in some isolated populations in Scotland, Ireland and the Alps. The broom hare Lepus castroviejoi, which is endemic to the Cantabrian Mountains, was the most massively affected because its aboriginal mtDNA lineage appears to have been totally replaced by mtDNA of L. timidus origin during two waves of hybridisation, the more recent one during the last deglaciation period and the earlier one during the Pleistocene (MeloFerreira et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Home-loving boreal hare mitochondria survived several invasions in Iberia: the relative roles of recurrent hybridisation and allele surfing

et al. 2013

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Genetic introgression from a resident species into an invading close relative can result from repeated hybridisation along the invasion front and/or allele surfing on the expansion wave. Cases where the phenomenon is massive and systematic, such as for hares (genus Lepus) in Iberia, would be best explained by recurrent hybridisation but this is difficult to prove because the donor populations are generally extinct. In the Pyrenean foothills, Lepus europaeus presumably replaced Lepus granatensis recently and the present species border is parallel to the direction of invasion, so that populations of L. granatensis in the contact zone represent proxies of existing variation before the invasion. Among three pairs of populations sampled across this border, we find less differentiation of mitochondrial DNA (mtDNA) across than along it, as predicted under recurrent hybridisation at the invasion front. Using autosomal microsatellite loci and X-and Y-linked diagnostic loci, we show that admixture across the border is quasi-absent, making it unlikely that lack of interspecific mtDNA differentiation results from ongoing gene flow. Furthermore, we find that the local species ranges are climatically contrasted, making it also unlikely that ongoing ecologydriven movement of the contact account for mtDNA introgression. The lack of mtDNA differentiation across the boundary is mostly due to sharing of mtDNA from a boreal species currently extinct in Iberia (Lepus timidus) whose mitochondria have thus remained in place since the last deglaciation despite successive invasions by two other species. Home-loving mitochondria thus witness past species distribution rather than ongoing exchanges across stabilised contact zones.

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Ancient introgression of Lepus timidus mtDNA into L. granatensis and L. europaeus in the Iberian Peninsula

Cited by 118 publications

References 31 publications

The ubiquitous mountain hare mitochondria: multiple introgressive hybridization in hares, genusLepus

The ubiquitous mountain hare mitochondria: multiple introgressive hybridization in hares, genusLepus

Identification of blood meals in field captured sand flies by a PCR-RFLP approach based on cytochrome b gene

Home-loving boreal hare mitochondria survived several invasions in Iberia: the relative roles of recurrent hybridisation and allele surfing

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