Ancient plant DNA in archaeobotany

Schlumbaum, Angela; Tensen, M.A.; Jaenicke-Després, Viviane

doi:10.1007/s00334-007-0125-7

Cited by 98 publications

(53 citation statements)

References 112 publications

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“…In the PET seeds the nuclear ITS region was however preserved, which opens up future possibilities to study potential hybridization and origin with nuclear markers (Clarke et al 2006). The results of the genetic analysis confirm the potential of waterlogged preserved plant material for aDNA analysis Table 1 ( Schlumbaum et al 2008) and the robustness of the primer for other geographic regions by Erickson et al (2005).…”

Section: Discussionsupporting

confidence: 48%

“…The degradation of the embryo containing the chloroplast DNA poses a frequent problem of typing cpDNA in archaeological seeds (Pollmann et al 2005;Schlumbaum et al 2008Schlumbaum et al , 2011. In the PET seeds the nuclear ITS region was however preserved, which opens up future possibilities to study potential hybridization and origin with nuclear markers (Clarke et al 2006).…”

Section: Discussionmentioning

confidence: 99%

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A short history of Lagenaria siceraria (bottle gourd) in the Roman provinces: morphotypes and archaeogenetics

Schlumbaum

Vandorpe

2011

Veget Hist Archaeobot

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Lagenaria siceraria (Molina) Standl. (bottle gourd) is pantropic and displays large variation in fruit and seed shape. Two subspecies are currently recognized: the African L. siceraria ssp. siceraria and the Asian L. siceraria ssp. asiatica. The Asian type of bottle gourd belongs to the earliest domesticated plants in the Americas. In Europe, bottle gourd only appears with some frequency from the Roman period onwards. The paper is the study of ancient DNA (aDNA) and seed morphology of one almost complete bottle gourd fruit from the Roman site of Oedenburg/Biesheim-Kunheim, France (1st century A.D.), and from individual seed finds from the Roman vicus of PetinescaVorderberg, Switzerland (3rd century A.D.), both recovered from waterlogged layers. Width and length measurements of seeds show large variation. Based on the index of width to length, seeds from both sites differ significantly (p \ 0.0001 Mann-Whitney) suggesting that there were different variants present north of the Alps. Genetically, the bottle gourd fruit from Roman Oedenburg/Biesheim-Kunheim and one commercial cultivar L. siceraria cv. 'Herkuleskeule' are of Asian origin as identified by three Asian and African specific chloroplast markers. These results support an early and longlasting presence of the Asian type of domestic bottle gourd in Europe. No chloroplast markers were found in the seeds from Petinesca-Vorderberg. However preserved nuclear high copy 5.8S rDNA fragments correctly matched to Cucurbitaceae, further supporting the evidence for preservation of DNA in waterlogged plant remains.

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Section: Discussionsupporting

confidence: 48%

Section: Discussionmentioning

confidence: 99%

A short history of Lagenaria siceraria (bottle gourd) in the Roman provinces: morphotypes and archaeogenetics

Schlumbaum

Vandorpe

2011

Veget Hist Archaeobot

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“…Allaby et al 1994;Jaenicke-Després et al 2003). The extraction of ancient DNA from plants (Gugerli et al 2005;Schlumbaum et al 2008), however, is relatively difficult and has yet to be successful for Australian or New Guinean samples. The preservation of DNA for long periods of time in a condition suitable for analysis requires very particular circumstances.…”

Section: Molecularmentioning

confidence: 99%

Archaeobotany in Australia and New Guinea: Practice, Potential and Prospects

Denham

Atchison

Austin

et al. 2009

Australian Archaeology

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Archaeobotany is the study of plant remains from archaeological contexts. Despite Australasian research being at the forefront of several methodological innovations over the last three decades, archaeobotany is now a relatively peripheral concern to most archaeological projects in Australia and New Guinea. In this paper, many practicing archaeobotanists working in these regions argue for a more central role for archaeobotany in standard archaeologicalpractice. An overview of archaeobotanical techniques and applications is presented, the potential for archaeobotany to address key historical research questions is indicated, and initiatives designed to promote archaeobotany and improve current practices are outlined.

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“…However, DNA extraction from dried wood stored for a long-term is more problematic (Finkeldey et al 2010;Abe et al 2011;Höltken et al 2011;Lowe and Cross 2011;Jiao et al 2012Jiao et al , 2014. After the death of an organism, hydrolytic and oxidative processes cause fragmentation and modification to its DNA (Pääbo et al 2004;Schlumbaum et al 2008). DNA increasingly degrades with age, resulting in the splitting of intact DNA into small fragments, ranging from 50 to 500 bp in length (Dumolin-Lapègue et al 1999;Deguilloux et al 2002;Speirs et al 2009;Jiao et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Extraction and amplification of DNA from aged and archaeological Populus euphratica wood for species identification

Jiao¹,

Liu²,

Jiang³

et al. 2015

Holzforschung

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Abstract:The wood samples of Populus euphratica Oliv. (Salicaceae) are common archaeological plant remains in the hot and arid regions of western China. However, it is difficult to identify P. euphratica wood based on traditional wood anatomical methods alone. DNA barcoding might provide a higher security for species identification. In this study, aged wood specimens stored for approximately 30, 60, and 80 years and archaeological wood up to 3600 years old were in focus to explore the potential of DNA extraction and PCR amplification for different-sized fragments, ranging between 100 and 800 bp, taken from wood stored for different periods. The results indicated that DNA fragments of more than 100 bp could be successfully retrieved from a wood specimen stored for about 80 years based on a modified Qiagen kit protocol. However, it was impossible to obtain DNA segments from the 3600-year-old wood according to the current extraction protocol. Moreover, it was deduced that two-stage PCR amplification could play a significant role in the analysis of DNA retrieved from aged wood materials. With the aid of phylogenetic analysis, based on the short DNA barcode rbcL-2 of 202 bp in length, it was possible to differentiate P. euphratica from the other species of the Populus genus.

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Ancient plant DNA in archaeobotany

Cited by 98 publications

References 112 publications

A short history of Lagenaria siceraria (bottle gourd) in the Roman provinces: morphotypes and archaeogenetics

A short history of Lagenaria siceraria (bottle gourd) in the Roman provinces: morphotypes and archaeogenetics

Archaeobotany in Australia and New Guinea: Practice, Potential and Prospects

Extraction and amplification of DNA from aged and archaeological Populus euphratica wood for species identification

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