Understanding past human settlement of inhospitable regions is one of the most intriguing puzzles in archaeological research, with implications for more sustainable use of marginal regions today. During the Byzantine period in the 4 th century CE, large settlements were established in the arid region of the Negev Desert, Israel, but it remains unclear why it did so, and why the settlements were abandoned three centuries later. Previous theories proposed that the Negev was a "green desert" in the early 1 st millennium CE, and that the Byzantine Empire withdrew from this region due to a dramatic climatic downturn. In the absence of a local climate archive correlated to the Byzantine/Early Islamic transition, testing this theory has proven challenging. We use stable isotopic indicators of animal dietary and mobility patterns to assess the extent of the vegetative cover in the desert. By doing so, we aim to detect possible climatic fluctuations that may have led to the abandonment of the Byzantine settlements. The findings show that the Negev Desert was not greener during the time period under investigation than it is today and that the composition of the animals' diets, as well as their grazing mobility patterns, remained unchanged through the Byzantine/Early Islamic transition. Favoring a nonclimatic explanation, we propose instead that the abandonment of the Negev Byzantine settlements was motivated by restructuring of the Empire's territorial priorities.Over the last 12,000 years, the Negev Desert in southern Israel has hosted an arid to hyper-arid climate, but despite the harsh living conditions, people have periodically established settlements here that have persisted for centuries 1,2 . Ongoing debate has seen archaeologists, historians, and climate scientists speculate whether the rise and fall of human habitation was caused by fluctuations in climatic conditions, or whether it was primarily driven by changes in the socio-political organization of the communities living in these environmentally marginal areas 3-7 . Palaeoenvironmental studies have contributed to this debate by providing a long-term perspective on climate fluctuations in the region. However, in order to understand the human response to these patterns on short-term (i.e. centennial) scales and ground the trends in specific micro-regional settings, archaeological proxies need to be studied from contexts that bracket the cultural events under consideration 8 .One of the most significant phases of population expansion in the Negev Desert started in the 3 rd century BCE with the establishment of the Nabatean kingdom. After the kingdom was annexed and subsumed into the Roman empire in 106 CE, it reached its height with the spread of Christianity during the Byzantine period in the 4 th -6 th century CE and was subsequently abandoned around the time of the Muslim conquest of the region in the 7 th century CE 9,10 . Between the 4 th -7 th centuries CE, five major Byzantine settlements -Elusa (Halutza), Subeita (Shivta), Nessana (Nitzana), Avdat (Oboda)...