ANCUT2, an Extracellular Cutinase from Aspergillus nidulans Induced by Olive Oil

Abstract

Cutinases are versatile carboxylic ester hydrolases with great potential in many biocatalytic processes, including biodiesel production. Genome sequence analysis of the model organism Aspergillus nidulans reveals four genes encoding putative cutinases. In this work, we purified and identified for the first time a cutinase (ANCUT2) produced by A. nidulans. ANCUT2 is a 29-kDa protein which consists of 255 amino acid residues. Comparison of the amino acid sequence of ANCUT2 with other microbial cutinase sequences revealed a high degree of homology with other fungal cutinases as well as new features, which include a serine-rich region and conserved cysteines. Cutinase production with different lipidic and carbon sources was also explored. Enzyme activity was induced by olive oil and some triacylglycerides and fatty acids, whereas it was repressed by glucose (1%) and other sugars. In some conditions, a 22-kDa post-translational processing product was also detected. The cutinase nature of the enzyme was confirmed after degradation of apple cutin.

Reducción de astringencia del pseudofruto de marañón (Anacardium occidentale L.) como alternativa para consumo humano

“…Furthermore, due to the difficulty of assessing the enzymatic activity of cutinase using radiolabeled cutin as substrate (Purdy & Kolattukudy, 1973;Davies et al, 2000). It was decided to detect cutinase activity by simpler methods using native cutins as substrate (Castro-Ochoa et al, 2012), and 4-nitrophenyl esters as commercial substrates (Degani et al, 2006). Degani (2015) reported that cutinase activity increases according to the amount of cutin added to the culture media.…”

“…Cutinases (E.C 3.1.1.73) are extracellular and inducible enzymes which break down cutin, the major component of vegetal cuticle in higher plants, which is composed of esterified fatty acids (C16 or C18) (Castro-Ochoa et al, 2012). Cutinases are predominantly produced by phytopathogenic fungi with the ability to grow on cutin as sole carbon source.…”

Enhancement of Biodegradable Plastic-degrading Enzyme Production from <i>Paraphoma</i>-like Fungus, Strain B47-9

“…Multiple cutinase genes have been identified in each of the following fungal species: F. solani f. sp. pisi 9 , A. oryzae 10 , and Aspergillus nidulans 11,12 . The expression of these genes is induced by polyesters such as biodegradable plastics or the plant polyester suberin 5, 11 13 .…”

Expression and characterization of a cutinase (AnCUT2) from Aspergillus niger

“…CAK41954.1) and A. flavus (GenBank accession no. EED52785.1) at the C-terminal [5]. In addition, the first author also wishes to thank the Government of the Kingdom of Saudi Arabia for awarding the fellowship for doctoral research.…”

“…Interestingly, this enzyme is also implicated in the pathogenicity of plant pathogenic fungi to break down plant cell walls releasing cutin monomers [4]. Cutinase belongs to the α/β hydroloase fold and serine esterase super-family with the classical Ser, His, and Asp triad [5,6].…”