Ancylomarina longa sp. nov., isolated from southern Okinawa Trough sediment and emended description of the family Marinifilaceae

Abstract

A Gram-stain-negative, obligately anaerobic, non-motile, non-spore-forming, long-rod-shaped and non-flagellated bacterial strain, designated T3-2 S1-C T , was isolated from a sediment sample collected at the Okinawa Trough. Phylogenetic analyses of 16S rRNA gene sequences and the whole genome revealed that strain T3-2 S1-C T was a member of the family Marinifilaceae and exhibited less than 95.1 % sequence similarities to the closely related type strains of the family Marinifilaceae. Optimal growth occurred at pH 7.0, 28 °C and in the presence of 3 % (w/v) NaCl. The isoprenoid quinone of strain T3-2 S1-C T was identified as menaquinone-7 (MK-7) and the predominant fatty acids (>10 %) were iso-C 15 : 0 (38.9 %) and anteiso-C 15 : 0 (11.6 %). The major polar lipids were one phosphatidylethanolamine, one phosphatidylmonomethylethanolamine, one aminolipids, two unidentified lipids and two unidentified phospholipids. The DNA G+C content of strain T3-2 S1-C T was 35.7 mol%. On the basis of the results of polyphasic analyses, strain T3-2 S1-C T is considered to represent a novel species of the genus Ancylomarina, for which the name Ancylomarina longa sp. nov. is proposed. The type strain is T3-2 S1-C T (=KCTC 15505 T =MCCC 1K01617 T).

Complete Genome Sequence of Lagilactobacillus salivarius VHProbi A17, Isolated from Fecal Samples

“…All procedures were in accordance with the Declaration of Helsinki. The inoculum was anaerobically incubated at 37°C for 48 h. A colony designated VHProbi A17 was picked and identified as the species Lagilactobacillus salivarius using 16S rRNA gene sequencing, as described by Fu et al ( 1 ). The strain was sequenced with Illumina HiSeq 2500 and Pacific Biosciences (PacBio) RS II platforms.…”

Complete Genome Sequence of Lactiplantibacillus plantarum VHProbi O10, Isolated from Kimchi

“…Then, 1 mL of dilution was spread onto the MRS agar plate and incubated under aerobic conditions at 37°C to enrich bacteria. After 48 h, a white colony designated VHProbi O10 was picked up and identified as the species Lactiplantibacillus plantarum using 16S rRNA sequencing as described by Fu et al ( 1 ). A combined sequencing strategy of Illumina HiSeq 2500 and the PacBio RS II single molecular real-time (SMRT) platform was used to sequence the genome of this strain.…”