Androgen-dmrt1positive feedback programs the rice field eel (Monopterus albus) sex transdifferentiation

Abstract

17The rice field eel Monopterus albus is a hermaphroditic protogynous fish species that 18 undergoes sex reversal from female to male. However, the potential mechanisms 19 underlying the process of sex transformation are still unclear. We analyzed and 20 compared the gene sequence of M. albus dmrt1 5′ upstream region and its potential 21 transcription factor binding sites with other known species and examined the in vitro 22 34 Androgens are synthesized in large amounts during sex reversal, leading to the 35 promotion of dmrt1 transcription, and thus, gonadal somatic cells transdifferetiation. 36 Overall, androgen-dmrt1 positive feedback programs the M. albus sex reversal. 37 38 39 111 other one on the antisense strand (-903 bp ~ -917 bp) (Supplementary Table 2). 112Histological change 113 After 6 hours of tissue culture, cells began to migrate from the periphery of the 114 130 Ozols' region, aromatic regions and heme-binding region ( Supplementary Fig. 3). We 131 compared the amino acid sequence of M. albus 11β-h to that in other species and 132 found 77% identity with Dicentrarchus labrax, 76% identity with Micropogonias 133 undulatus and 75% identity with Parajulis poecilepterus and Odontesthes bonariensis. 134 The phylogenetic tree analysis showed that the 11β-h of M. albus and Epinephelus 135 coioides, P. poecilepterus, D. labrax, M. undulatus, O. bonariensis, O. latipes and O. 136