Androgen levels during sequential insulin euglycemic clamp studies in patients with polycystic ovary disease

Micić, Dragan; Popović, Vera; Nesović, M; Sumarac, Mirjana; Dragasević, M; Kendereški, Aleksandra; Marković, Dragan; Djordjević, Predrag; Manojlović, D; Micić, Jelena

doi:10.1016/0022-4731(88)90344-5

Cited by 38 publications

(18 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The fact that circulating testosterone levels were not related to any of these variables argues against a causative role for testosterone in promoting greater adiposity. Instead, our data favor the interpretation that increasing amounts of body fat may promote increased circulating testosterone, possibly through an effect on tissue insulin sensitivity [18, 19]. Importantly, we cannot discount the possibility that testosterone promotes fat gain via modulation of energy intake, which some animal models have suggested [20, 21].…”

Section: Discussioncontrasting

confidence: 57%

Relationship of androgens to body composition, energy and substrate metabolism and aerobic capacity in healthy, young women

2011

View full text Add to dashboard Cite

Objective To evaluate the role of physiologic levels of androgens and their precursors in the regulation of body composition, energy and substrate metabolism and aerobic capacity in healthy, cycling, premenopausal women. Experimental We evaluated 30 young (27 ± 1 yr) premenopausal, non-obese (23 ± 0.5 kg/m2), normally-cycling women, without clinical or chemical evidence of hyperandrogenism or hyperinsulinemia, for parameters of total and regional body composition, glucose tolerance, aerobic capacity and resting energy expenditure and substrate oxidation. Serum was assayed for androgens and androgen precursors by techniques optimized to assess the low androgen levels in this population. Results Higher serum testosterone levels correlated with greater fat mass (r= .377; p=0.04), but not abdominal adiposity or other metabolic/physiologic variables. Additionally, dehydroepiandrosterone (DHEA) was negatively related to visceral fat content (r=−.569; p=0.02). Other serum androgens did not correlate with total or regional adiposity, skeletal muscle mass, aerobic capacity, glucose tolerance, or resting energy and substrate metabolism. Conclusion In this group of non-obese, premenopausal women with no clinical or chemical evidence of hyperandrogenemia, serum testosterone levels were positively related with fat mass, but not with abdominal adiposity; whereas, DHEA was negatively related to visceral adiposity. Our data suggest that within the normal physiologic range, testosterone is a predictor of overall adiposity, but that this effect does not appear to be associated with concomitant alterations in resting energy or substrate metabolism that could predispose to weight gain.

show abstract

Section: Discussioncontrasting

confidence: 57%

Relationship of androgens to body composition, energy and substrate metabolism and aerobic capacity in healthy, young women

2011

View full text Add to dashboard Cite

show abstract

“…As a further indication of the link between hyperinsulinemia and ovarian steroidogenesis, hyperinsulinemic states induced by hyperinsulinemic-euglycemic clamps lead to increased ovarian androgen production in lean women with PCOS, demonstrating an acute induction of ovarian steroidogenesis by insulin (41). …”

Section: Discussionmentioning

confidence: 99%

Reproductive Tissues Maintain Insulin Sensitivity in Diet-Induced Obesity

DiVall

Wondisford

et al. 2011

Diabetes

View full text Add to dashboard Cite

Reproductive dysfunction is associated with obesity. We previously showed that female mice with diet-induced obesity (DIO) exhibit infertility and thus serve as a model of human polycystic ovary syndrome (PCOS). We postulated that differential insulin signaling of tissues leads to reproductive dysfunction; therefore, a comparison of insulin signaling in reproductive tissues and energy storage tissues was performed. Pituitary-specific insulin receptor knockout mice were used as controls. High-fat diet–induced stress, which leads to insulin resistance, was also investigated by assaying macrophage infiltration and phosphorylated Jun NH2-terminal kinase (pJNK) signaling. In lean mice, reproductive tissues exhibited reduced sensitivity to insulin compared with peripheral metabolic tissues. However, in obese mice, where metabolic tissues exhibited insulin resistance, the pituitary and ovary maintained insulin sensitivity. Pituitaries responded to insulin through insulin receptor substrate (IRS)2 but not IRS1, whereas in the ovary, both IRS1 and IRS2 were activated by insulin. Macrophage infiltration and pJNK signaling were not increased in the pituitary or ovary of lean mice relative to DIO mice. The lack of inflammation and cytokine signaling in the pituitary and ovary in DIO mice compared with lean mice may be one of the reasons that these tissues remained insulin sensitive. Retained sensitivity of the pituitary and ovary to insulin may contribute to the pathophysiology of PCOS.

show abstract

“…There was significant T increase at the end of insulin infusion. There were no significant changes in DHEAS levels during clamp studies [25,26] .…”

Section: Discussionmentioning

confidence: 99%

“…Hyperinsulinemia acutely stimulates hypothalamic pituitary axis secretory activity during euglycemia [33][34][35][36][37][38][39] . During the euglycemic clamp, the high rate insulin infusion increased plasma ACTH levels, whereas plasma ACTH levels remained essentially unchanged during the low-insulin condition.…”

Section: Discussionmentioning

confidence: 99%

Assessment of Insulin Resistance in the Idiopathic Hirsutism

Saraç¹,

Saygılı²,

Ozgen³

et al. 2006

Gynecol Obstet Invest

View full text Add to dashboard Cite

Background: Idiopathic hirsutism (IH) is the second most common cause of hirsutism, after polycystic ovary syndrome and occurs in about 15% of hirsute women. There are not many studies showing whether patients with IH also have insulin resistance. In the present study, we aimed to investigate the insulin sensivity in IH with non-obese and changing hormone levels during the hyperinsulinemic-euglycemic clamp. Methods: Twenty (20) non-obese women with IH (Group I) ranging in age from 20 to 30 (mean 25 ± 5) years were studied. Hirsutism in women with normal testosterone (T) levels and regular menstrual cycles is as defined IH. Twenty (20) healthy women (mean age 23 ± 2 years) (Group II) were included in this study as the control group. Insulin sensitivity was assessed with modified euglycemic insulin clamp technique. Samples of prolactin, luteinizing hormone (LH), follicle-stimulating hormone, adrenocorticotropic hormone (ACTH), dehydroepiandrosterone sulfate, cortisol, estradiol, progesterone, 17-OH progesterone (17-OHP), total T, and free T (FT) were obtained at baseline and at 2nd hour during clamp. Results: Steady-state (120 min) glucose disposal rates were higher in Group II than Group I (7.51 ± 0.83 vs. 5.76 ± 1.89 mg/kg/min). Mean FT, ACTH, cortisol, LH, prolactin and 17-OHP levels were found to have decreased statistically significantly (p < 0.05) in Group I. Mean FT, ACTH, and prolactin levels were found to have decreased statistically significantly (p < 0.05) in Group II during the clamp. Mean baseline levels of FT, LH and prolactin were greater in women with hirsutism than in the control subjects (p < 0.05). Insulin mediated glucose disposal was lower in the normal weight women with IH than in those without hirsutism. Conclusions: Mean FT, 17-OHP and dehydroepiandrosterone sulfate levels decreased during euglcemic-hyperinsulinemic clamp in IH.

show abstract

Androgen levels during sequential insulin euglycemic clamp studies in patients with polycystic ovary disease

Cited by 38 publications

References 12 publications

Relationship of androgens to body composition, energy and substrate metabolism and aerobic capacity in healthy, young women

Relationship of androgens to body composition, energy and substrate metabolism and aerobic capacity in healthy, young women

Reproductive Tissues Maintain Insulin Sensitivity in Diet-Induced Obesity

Assessment of Insulin Resistance in the Idiopathic Hirsutism

Contact Info

Product

Resources

About