Androgen metabolism in oyster Crassostrea gigas: evidence for 17β-HSD activities and characterization of an aromatase-like activity inhibited by pharmacological compounds and a marine pollutant

Curieux-Belfond, Olivier Le; Moslemi, Safa; Mathieu, Michel; Séralini, Gilles‐Éric

doi:10.1016/s0960-0760(01)00109-1

Cited by 71 publications

(40 citation statements)

References 38 publications

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“…Usually, incubation of tissue extracts with labeled vertebrate-type precursors have been undertaken and although information is still fragmentary and comes from different species, most of the crucial enzymatic activities have been detected: steroid desmolase in Placopecten magellanicus (Idler et al 1969), 17b-hydroxysteroid dehydrogenase (17b-HSD) in Crassostrea virginica (Hathaway 1965), and C. gigas (Mori et al 1966;Le Curieux-Belfond et al 2001), 17b-HSD in Patinopecten yessoensis (Matsumoto et al 1997), C-20-22 lyase, 3b-HSD, 17a-hydroxylase, C-17-20 lyase, 17b-HSD, and 5a reductase in Mytilus edulis (DeLongcamp et al 1974), 17b/3b HSD and 5a reductase in the freshwater gastropod Marisa cornuarietis by (Janer et al 2005), C20-22-lyase, 3b-HSD/D5-4 isomerase, 17a-hydroxylase, C17-20-lyase, 17b-HSD, and 20-HSD in the cuttlefish Sepia officinalis (Carreau and Drosdowsky 1977), 3b-HSD/D5-4 isomerase, in Octopus vulgaris (D'Aniello et al 1996).…”

Section: Evidence For Endogenous Productionmentioning

confidence: 99%

Steroids in aquatic invertebrates

2007

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Steroid molecules are present in all invertebrates, and some of them have established hormonal roles: this is the case for ecdysteroids in arthropods and, to a lesser extent, for vertebrate-type steroids in molluscs. Steroids are not only hormones, they may also fulfill many other functions in chemical communication, chemical defense or even digestive physiology. The increasing occurrence of endocrine disruption problems caused by environmental pollutants, which interfere in particular with reproductive physiology of vertebrates but also of invertebrates has made necessary to better understand the endocrine physiology of the latter and the role of steroids in these processes. So many attempts are being made to better understand the endocrine roles of steroids in arthropods and molluscs, and to establish whether they also fulfill similar functions in other invertebrate phyla. At the moment, both the precise identification of these steroids, the determination of their origin (endogenous versus exogenous) and of their mechanism of action are under active investigation. This research takes profit of the development of genome sequencing programs on many invertebrate species, which allow the identification of receptors and/or biosynthetic enzymes, when related to their vertebrate counterparts, but the story is not so simple, as will be exemplified by estrogen receptors of molluscs.

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Section: Evidence For Endogenous Productionmentioning

confidence: 99%

Steroids in aquatic invertebrates

2007

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“…This enzyme requires NADPH as a cofactor and involves hydroxylations and dehydrations that culminate in aromatization of the A ring of the androgens (Simpson and Davis 2001). Aromatase activity has been reported in molluscs (Matsumoto et al 1997;Le Curieux-Belfond et al 2001;Horiguchi et al 2004), and in echinoderms (Lavado et al 2006c). However, the aromatization rates reported are rather close to the detection limit of the methods used to assess the activity.…”

Section: Cytochrome P450-dependent Biotransformations: Aromatization mentioning

confidence: 99%

“…However, the aromatization rates reported are rather close to the detection limit of the methods used to assess the activity. Two methods widely used in the analysis of aromatase activity in invertebrates include the tritiated water release assay, which is based on the quantification of the tritiated water released during [ 3 H]-androstenedione aromatization (Matsumoto et al 1997;Le Curieux-Belfond et al 2001;Lavado et al 2006a), and the analysis by GC-MS of the differential estradiol levels before and after incubating tissue slices with testosterone (Horiguchi et al 2004).…”

Section: Cytochrome P450-dependent Biotransformations: Aromatization mentioning

confidence: 99%

Sex steroids and potential mechanisms of non-genomic endocrine disruption in invertebrates

2007

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The review reports on the presence and metabolism of sex steroids in several invertebrate species and provides detailed information on possible mechanisms of endocrine disruption other than the interaction with nuclear receptors. The presence of most vertebrate sex steroids in invertebrate tissues has been demonstrated by liquid or gas chromatography coupled to mass spectrometry. In addition, enzymatic pathways involved in the steroidogenic pathway have been described in at least some invertebrate phyla. Some endocrine disruptors induce alterations in these metabolic pathways and might lead to changes in steroid levels. Growing evidence suggests that estradiol can act through non-genomic pathways in molluscs, and that xenobiotics can as well interfere in these signalling cascades. In spite of these recent advances, most question marks on the action and function of sex steroids in invertebrates remain to be answered.

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“…Recently, it was also reported that TBT altered steroid hormone metabolism and inhibited the activity of aromatase and other P450-enzymes in rats, marine species and human granulose-like tumor cells [18,23,24], therefore in mice, TBTO might also affect the endocrine system via the steroidogenesis pathway with toxic effects on the testicular function. In this study, we also tried to measure the serum testosterone level, however, we c ou ldn 't fin d t he exa c t ten den cy of ser um testosterone level in the TBTO-treated mice because of wide individual differences (data not shown).…”

Section: Discussionmentioning

confidence: 99%

Toxicity of the Tributyltin Compound on the Testis in Premature Mice.

Kumasaka

Miyazawa

Fujimaki

et al. 2002

Journal of Reproduction and Development

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Abstract. In this study, the toxicity of bis (tri-n-butyltin) oxide (TBTO), a marine pollutant, in premature mouse testes and the correlation of a total tin concentration with toxicity were examined. Mice were treated by oral administration twice a week for four weeks from five weeks of age at doses of 0 mg/kg (control), 0.4 mg/kg, 2.0 mg/kg, or 10 mg/kg, and sacrificed on the day following the final administration. The control mice were administered with distilled water containing 0.2% ethanol as vehicle. Removed testes were used for determination of the testicular sperm head counts and for histological study or determination of the total tin concentration. The sperm head count was significantly decreased in the 2.0 mg/kg and 10 mg/kg administration groups, although the testicular weights of both groups were unchanged compared to that of the control. The histological study revealed that TBTO caused vacuolization of Sertoli cells in several seminiferous tubules that failed to organize, however, the frequencies of occurrence were low. The total tin concentration in the testis increased in a dose-dependent manner in inverse proportion to the reduction of sperm head counts. These results suggest that TBTO is potentially an anti-testicular compound in mice.

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Androgen metabolism in oyster Crassostrea gigas: evidence for 17β-HSD activities and characterization of an aromatase-like activity inhibited by pharmacological compounds and a marine pollutant

Cited by 71 publications

References 38 publications

Steroids in aquatic invertebrates

Steroids in aquatic invertebrates

Sex steroids and potential mechanisms of non-genomic endocrine disruption in invertebrates

Toxicity of the Tributyltin Compound on the Testis in Premature Mice.

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