Androgen Receptors in Normal and Neoplastic Laryngeal Tissue

Veĕerina-Volić, Santa; Romić-Stojković, Ranka; Krajina, Z; Gamulin, Stjepan

doi:10.1001/archotol.1987.01860040073021

Cited by 16 publications

(8 citation statements)

References 6 publications

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“…Furthermore, our immunohistochemical analysis corroborates the biochemical findings, as we did not find any specific staining for AR, ER or PR. The discrepancy between our immunohistochemical results and some biochemical analyses (such as Saez and Sakai [24], Jing-qing et al [12] and VecerinaVolic et al [30]) may be explained by a contamination of the tumor homogenates by the serum of the patients. A similar explanation has been given for the biochemical analysis of hormone receptors in renal cell carcinomas, where Krech et al [16] suggested this explanation, which takes into consideration that serum may contain small amounts of SHR.…”

Section: Discussioncontrasting

confidence: 89%

“…Similar results have also been published by VecerinaVolic et al, who found a significant concentration of cytoplasmatic ARs in female neoplastic tissue and in 6 out of 16 male patients. Also, nuclear receptors were detected in five out of nine specimens of male laryngeal carcinoma [30].…”

Section: Discussionmentioning

confidence: 93%

“…The fact that the larynx is a secondary sex organ and undergoes developmental and physiologic changes during puberty and throughout the menstrual cycle, combined with the high rate of laryngeal carcinomas in men, has led to the suggestion that laryngeal carcinoma might be a sexhormone-dependent tumor similar to prostate and mammary carcinoma [18,24,26,28,30]. In those sex-dependent carcinomas, the amount of expressed sex-hormone receptors (SHR) is already a well-established, independent prognostic factor for the patients, providing important information for tumor treatment with antiandrogen or antiestrogen drugs.…”

Section: Introductionmentioning

confidence: 99%

“…There has been speculation that the increased frequency of laryngeal carcinomas in men may be associated with an enhanced activation of androgen-receptors (AR) in the larynx [18,24,28,30]. It is known that androgens control the growth of the laryngeal muscles [11], while testosterone causes temporary hypertrophy of the squamous epithelium of the vocal cords [11].…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, the level of serum testosterone in patients with laryngeal carcinomas was found to be increased [12]. Vecerina-Volic et al identified cytoplasmatic and nuclear ARs in laryngeal carcinomas [30]. Molteni et al observed high levels of estrogen-receptor (ER) protein [20] in several head and neck tumors.…”

Section: Introductionmentioning

confidence: 99%

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Analysis of sex-hormone-receptor expression in laryngeal carcinoma

Hagedorn¹,

Nerlich

2002

European Archives of Oto-Rhino-Laryngology

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There are significant differences in how laryngeal cancer affects the two genders, with cases occuring predominantly in males. This has been speculated to result from the different susceptibilities of the tumor cells to steroid sex hormones. Since the sex hormone action is mediated by specific cellular receptors, several previous studies have analyzed the presence of these sex-hormone receptors. However, the data on the receptor status for androgen, estrogen and progesterone receptors in laryngeal carcinomas are controversial. Since some authors have suggested antiandrogen or antiestrogen therapy as an adjuvant treatment for laryngeal carcinoma, we performed a comprehensive study using immunomorphological and biochemical techniques on both in-vivo and in-vitro tumor cells in order to clarify whether major sex-hormone receptors are present or not. We performed an immunohistochemical analysis on 13 patients with laryngeal carcinomas, 8 males and 5 females, using monoclonal antibodies against androgen, estrogen and progesterone receptors. In addition, the presence of estrogen and progesterone receptors was tested biochemically using an enzyme immune assay (EIA). Furthermore, we analyzed immunohistochemically the expression of the hormone receptors in five keratinocyte cell lines originating from laryngeal carcinomas. In all of our tumor samples, as well as in the five tumor cell lines tested, we did not detect any specific immunohistochemical staining for androgen, estrogen and progesterone receptors in the laryngeal carcinoma cells when compared to the positive controls of breast and prostate cancer samples. Similarly, the biochemical analysis did not demonstrate any significant amount of receptor protein in the analyzed cases. In conclusion, the absence of male and female sex-hormone receptors strongly argues against laryngeal carcinomas being sex-hormone-dependent tumors. Therefore, we do not see any rational indication to use a specific antiandrogen or antiestrogen therapy for the adjuvant treatment of laryngeal carcinomas.

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Section: Discussioncontrasting

confidence: 89%

Section: Discussionmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Analysis of sex-hormone-receptor expression in laryngeal carcinoma

Hagedorn¹,

Nerlich

2002

European Archives of Oto-Rhino-Laryngology

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Sex steroid receptors in intracranial tumors

Stojković

Jovančević

et al. 1990

Cancer

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Estrogen (ER), progestin (PGR), and androgen (AR) receptors were assayed in cytosols prepared from 38 various intracranial tumors. The receptors were in the following proportions (number of receptor-positive/number of tumors examined): meningiomas were positive for PGR (4/6) and AR (2/5); glioblastomas were also positive for PGR (3/21) and AR (7/21); astrocytomas were positive only for PGR (4/5); and oligodendrogliomas only for AR. In two hamartomas AR was present, while in one chordoma both PGR and AR were present. In this latter tumor ER were not assayed due to insufficient material. The receptors were present in concentrations between 10 and 20 fmol/mg protein. Exceptions were two meningiomas and a chordoma with a high concentration of PGR and AR. Our results support the notion that a proportion of intracranial tumors contains sex steroid receptors, and some of these tumors might be hormonally dependent.

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Receptors for epidermal growth factor and steroid hormones in primary laryngeal tumors

Scambia¹,

Panici²,

Battaglia³

et al. 1991

Cancer

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The authors investigated the presence of epidermal growth factor (EGF-R), estrogen (ER), progesterone (PR), and androgen (AR) receptors in 42 laryngeal squamous cell carcinomas and 20 normal laryngeal mucosa specimens. EGF-R were expressed in all tumor specimens analyzed, with significantly higher levels in tumor samples compared with normal mucosa (P less than 0.05). The immunohistochemical study demonstrated the presence of EGF-R in basal and parabasal cells of normal mucosa and in most of the cancer cells. There was no correlation between EGF-R and tumor localization or T classification. On the other hand, the authors did find an interesting correlation between EGF-R levels and grading, EGF-R levels being significantly higher in G3 than in G1-G2 tumors. Moreover, the three patients whose tumors expressed the highest EGF-R levels had disease recur within 8 months. Among tumor specimens, 9.5%, 35.7%, and 9.5% expressed very low but detectable ER, PR, and AR levels, respectively. No statistically significant difference was found between the levels of steroid receptors in the tumor and normal mucosa specimens, and neither was there any correlation of ER, PR, and AR with the pathologic findings. The authors' results suggest that the EGF-R system may play a role in regulating the growth of laryngeal cancer. Additional studies should demonstrate whether, as in other tumor types, EGF-R expression may have prognostic significance in human laryngeal cancer.

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Androgen Receptors in Normal and Neoplastic Laryngeal Tissue

Cited by 16 publications

References 6 publications

Analysis of sex-hormone-receptor expression in laryngeal carcinoma

Analysis of sex-hormone-receptor expression in laryngeal carcinoma

Sex steroid receptors in intracranial tumors

Receptors for epidermal growth factor and steroid hormones in primary laryngeal tumors

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