Androgen-Regulated Genes in the Murine Epididymis1

Chauvin, Theodore R.; Griswold, Michael D.

doi:10.1095/biolreprod.103.026302

Cited by 84 publications

(48 citation statements)

References 42 publications

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“…Expression of proteins secreted from epididymal epithelium are often regulated by testicular factors or androgen (Chauvin and Griswold, 2004;Johnston et al, 2005). We analyzed the expression of BPI in caput and caudal epididymides of rats that had been bilaterally castrated and reared for 7 days.…”

Section: Effects Of Castration On the Expression Of Bpimentioning

confidence: 99%

Bactericidal/Permeability‐Increasing Protein Is Associated With the Acrosome Region of Rodent Epididymal Spermatozoa

Yano

Matsuyama

Kaneko

et al. 2010

Journal of Andrology

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To elucidate the molecular mechanisms involved in sperm maturation during epididymal transit, we intended to isolate secretory molecules that are region-specifically expressed along the epididymis and secreted into the lumen of epididymal ducts. By using differential display screening and DNA sequence analyses, we isolated a rat bactericidal/permeability-increasing protein (BPI) possessing a signal sequence at its N-terminal, which was expressed in the caput region of epididymis, but not in the caudal region. Reverse transcription polymerase chain reaction analysis and in situ hybridization showed that rat BPI messenger RNA (mRNA) was highly expressed in caput epididymal epithelium and that its expression level was developmentally up-regulated. Confocal laser scanning microscopy with the anti-BPI antibody revealed that in both rats and mice, BPI protein was detected on granulelike structures in the lumen of both caput and cauda epididymal ducts, as well as at the sperm surface covering the acrosome region in spermatozoa freshly isolated from epididymis. Acrosome reaction induced by calcium ionophore A23187 in vitro brought about the disappearance of BPI on mouse spermatozoa. These data suggested that BPI, which is synthesized in caput epididymis and secreted into the lumen, is associated with not only the granulelike structures, but also the sperm surface covering the acrosome region, and that BPI bound to the acrosome region is extinguished by acrosome reaction. Possibly BPI bound to the sperm surface covering the acrosome region in rodent spermatozoa is involved in sperm maturation or fertilization.

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Section: Effects Of Castration On the Expression Of Bpimentioning

confidence: 99%

Bactericidal/Permeability‐Increasing Protein Is Associated With the Acrosome Region of Rodent Epididymal Spermatozoa

Yano

Matsuyama

Kaneko

et al. 2010

Journal of Andrology

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“…Many genes show androgen-dependent expression patterns as revealed by individual studies and the comprehensive profiling of gene expression changes before and after orchidectomy in the rat [13][14][15][16]. In the mouse, by castration and dihydrotestosterone (DHT) replacement, the androgen-responsive genes including up-or down-regulated by DHT were identified [17]. Previous studies reported changes in gene expression during epididymal development and aging including 5a-reductases, E-cadherin, occludin, and ZO-1 which decline during aging [18].…”

Section: Introductionmentioning

confidence: 99%

Comparative profiling of genes and miRNAs expressed in the newborn, young adult, and aged human epididymides

Zhang¹,

Liu²,

Zhang³

et al. 2010

ABBS

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To understand roles of transcriptional factors and miRNAs in regulating gene expression in the epididymis from postnatal development through aging, systematic profiling of genes and miRNAs expressed in the newborn, young adult, and aged human epididymides was performed by cDNA array and miRNA array analysis, respectively. The newborn human epididymis expressed the fewest mRNAs but the largest number of miRNAs, whereas the adult and aged epididymides expressed the most mRNAs but the fewest miRNAs, a negative correlation between mRNAs and miRNA during aging. By integrative analysis, a set of miRNA targets were predicted based on the miRNA and cDNA arrays. In the newborn epididymis, 127 miRNAs were exclusively or preferentially expressed but only 3 and 2 miRNAs showed an age-enriched expression pattern in the adult and aged epididymides, respectively. This study provides a basic database as well as new insights and foundations for further studies on the complex regulation of gene expression in the epididymis.

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“…Here, we detected four epididymal androgen-responsive genes, namely Adam7, Fkbp5, Gldc, and Gpx5 (36,37). No miR-29a-and miR-29b-binding sites on the 3Ј-UTR of these four genes were predicted using the on-line software miRWalk, indicating that they should not be direct targets of miR-29a and miR-29b.…”

Section: Androgen Negatively Regulates Mir-29a Expression In Epi-mentioning

confidence: 96%

An Androgen Receptor-MicroRNA-29a Regulatory Circuitry in Mouse Epididymis

Wei

Yao

et al. 2013

Journal of Biological Chemistry

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Background: Abnormally up-regulated miR-29a is associated with several diseases, so miR-29a repression is important for homeostasis. Results: miR-29a was repressed by androgen system and inversely inhibited AR expression by targeting IGF1 and CDC42/p85␣-p53 pathways in mouse epididymis. Conclusion:There is an androgen receptor-miR-29a regulatory circuitry in mouse epididymis. Significance: The mutual repression between miR-29a and AR may be important for epididymal development and functions.

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Androgen-Regulated Genes in the Murine Epididymis1

Cited by 84 publications

References 42 publications

Bactericidal/Permeability‐Increasing Protein Is Associated With the Acrosome Region of Rodent Epididymal Spermatozoa

Bactericidal/Permeability‐Increasing Protein Is Associated With the Acrosome Region of Rodent Epididymal Spermatozoa

Comparative profiling of genes and miRNAs expressed in the newborn, young adult, and aged human epididymides

An Androgen Receptor-MicroRNA-29a Regulatory Circuitry in Mouse Epididymis

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