Androgen Signaling in Esophageal Adenocarcinoma Cell Lines In Vitro

Palethorpe, Helen M.; Drew, Paul A.; Smith, Eric

doi:10.1007/s10620-017-4794-5

Cited by 22 publications

(23 citation statements)

References 51 publications

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“…EAC has one of the highest male-to-female ratios reported for cancers of non-reproductive organs, 7–10 to 1, significantly higher than for the major risk factors [19].…”

Section: Esophageal Adenocarcinomamentioning

confidence: 91%

Esophageal cancer research today and tomorrow: Lessons from algae and other perspectives

Tiasto¹,

Mikhaĭlova²,

Gulaia³

et al. 2018

AIMS Genetics

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Esophageal cancer is an increasing concern due to poor prognosis, aggressive disease modalities, and a lack of efficient therapeutics. The two types of esophageal cancer: esophageal squamous cell carcinoma (ESCC) and esophageal adenocarcinoma (EAC) are responsible for an estimated 450,000 annual deaths, with over 457,000 new patients diagnosed in 2015, making it the eighth most prevalent and the 10th most fatal cancer worldwide. As esophageal cancer prevalence continues to increase, and so does the pressing need for the development of new and effective strategies for the early diagnostics, prevention, and treatment of this cancer, as well for building the innovative research tools to understand the affected molecular mechanisms. This short review summarizes the current statistics and recent research of the problems and solutions related to the esophageal cancer, and offer a brief overview of its epidemiology, molecular alterations, and existing biomedical tools. We will discuss currently available research tools and discuss selected approaches we deem relevant to find new model systems and therapies for the future with the special focus on novel opportunities presented by the unique molecules found in algae, namely carbohydrates and lipids. Their remarkable chemical variability is connected to their striking structural and functional properties, which combined with the relative novelty of these compounds to cancer biology, warrants interest of the wide biomedical community to these molecules, especially in the esophageal cancer theory and practice.

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“…EAC has one of the highest male-to-female ratios reported for cancers of non-reproductive organs, 7–10 to 1, significantly higher than for the major risk factors [19].…”

Section: Esophageal Adenocarcinomamentioning

confidence: 91%

Esophageal cancer research today and tomorrow: Lessons from algae and other perspectives

Tiasto¹,

Mikhaĭlova²,

Gulaia³

et al. 2018

AIMS Genetics

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“…Cell growth was determined by crystal violet assay as described previously [ 18 ]. Briefly, 10 3 cells per well were seeded into 96-well plates and cultured for 24 h. Culture medium without cells was added to 6 wells to serve as background controls for nonspecific binding of the crystal violet dye.…”

Section: Methodsmentioning

confidence: 99%

The Purified Extract from the Medicinal Plant Bacopa monnieri, Bacopaside II, Inhibits Growth of Colon Cancer Cells In Vitro by Inducing Cell Cycle Arrest and Apoptosis

Smith

Palethorpe

Tomita

et al. 2018

Cells

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Aquaporin-1 (AQP1), a transmembrane pore-forming molecule, facilitates the rapid movement of water and small solutes across cell membranes. We have previously shown that bacopaside II, an extract from the medicinal herb Bacopa monnieri, blocks the AQP1 water channel and impairs migration of cells that express AQP1. The aim of this study was to further elucidate the anti-tumour potential of bacopaside II in colon cancer cells. Expression of AQP1 in HT-29, SW480, SW620 and HCT116 was determined by quantitative PCR and western immunoblot. Cells were treated with bacopaside II, and morphology, growth, autophagy, cell cycle and apoptosis assessed by time-lapse microscopy, crystal violet, acridine orange, propidium iodide (PI) and annexin V/PI staining respectively. AQP1 expression was significantly higher in HT-29 than SW480, SW620 and HCT116. Bacopaside II significantly reduced growth at ≥20 µM for HT-29 and ≥15 µM for SW480, SW620 and HCT116. Inhibition of HT-29 at 20 µM was primarily mediated by G0/G1 cell cycle arrest, and at 30 µM by G2/M arrest and apoptosis. Inhibition of SW480, SW620 and HCT116 at ≥15 µM was mediated by G2/M arrest and apoptosis. These results are the first to show that bacopaside II inhibits colon cancer cell growth by inducing cell cycle arrest and apoptosis.

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“…Importantly, individuals with intact copies of UGT2B17 locus have substantially higher testosterone to epitestosterone ratio (T/E) in urine [33]. In this light, it is important to note that EA has marked male dominance with a male-to-female ratio of up to 9:1, pointing that the hormonal balance may play a specific role in the pathogenesis of EA [35,36]. Recent studies show that the cell lines derived from EA respond to stimulation with androgens [37].…”

Section: Discussionmentioning

confidence: 99%

UGT2B17 and miR-224 contribute to hormone dependency trends in adenocarcinoma and squamous cell carcinoma of esophagus

et al. 2019

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Esophageal squamous cell carcinoma (ESCC) and esophageal adenocarcinoma (EA) are the two main subtypes of esophageal cancer. Genetics underpinnings of EA are substantially less understood than that of ESCC. A large-scale relation data analysis was conducted to explore the genes implicated with either EA or ESCC, or both. Each gene linked to ESCC but not EA was further explored in mega-analysis of six independently collected EA RNA expression datasets. A multiple linear regression (MLR) model was built to study the possible influence of sample size, population region, and study date on the gene expression data in EA. Finally, a functional pathway analysis was conducted to identify the possible linkage between EA and the genes identified as novel significant contributors. We have identified 276 genes associated with EA, 1088 with ESCC, with a significant (P<5.14e-143) overlap between these two gene groups (n=157). Mega-analysis showed that two ESCC-related genes, UGT2B17 and MIR224, were significantly associated with EA (P-value <1e-10), with multiple connecting pathways revealed by functional analysis. ESCC and EA share some common pathophysiological pathways. Further study of UGT2B17 and MIR224, which are differentially dysregulated in ESCC and EA tumors, is warranted. Enhanced expression of UGT2B17 and the lack of miR-224 signaling may contribute to the responsiveness of EA to the male sex steroids.

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Androgen Signaling in Esophageal Adenocarcinoma Cell Lines In Vitro

Cited by 22 publications

References 51 publications

Esophageal cancer research today and tomorrow: Lessons from algae and other perspectives

Esophageal cancer research today and tomorrow: Lessons from algae and other perspectives

The Purified Extract from the Medicinal Plant Bacopa monnieri, Bacopaside II, Inhibits Growth of Colon Cancer Cells In Vitro by Inducing Cell Cycle Arrest and Apoptosis

UGT2B17 and miR-224 contribute to hormone dependency trends in adenocarcinoma and squamous cell carcinoma of esophagus

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