Androgenesis in Hexaploid Spring Wheat F₂ Populations and Their Parents Using a Multiple‐Step Regeneration System

Abstract

The main goals of the wheat androgenesis experiment were to study the main phenomena of in vitro androgenesis in anther culture of Fj populations (10) and their parents (6), to compare the usage of P-4 and G-17 media for the formation of embryo/callus and to demonstrate a new plant regeneration system.The P-4 induction medium was found to be significantly better than the G-17 in the number of responsive anthers (RA) and calli induced (GI) at the 1 % and 0.1 % level, respectively. Genotypic effect was evident in both RA and GI. The yields of F2 populations in RA and GI were significantly higher than those of their parents regarding both media. The data confirmed the existence of heterosis for RA and GI in F2 populations.The ratio of green/albino plant regeneration was more favourable in the G-17 derived embryo/calli than in the P-4 derived ones.The frequency of plantlet regeneration was enhanced in the group of unresponsive calli by the application of the multiple-step regeneration system. In this system the calli lacking well developed morphogenic structure were transferred to a new regeneration medium, containing a higher concentration of the same cytokinin, other cytokinin or basic medium, before the occurrence of irreversible changes in their physiology.

Inheritance of anther culture derived green plantlet regeneration in wheat (Triticum aestivum L.)

“…The correlation between anther culture response and callus induction frequency or embryo induction frequency was high (r --0.95 and 0.94, respectively), similar to the results that were found by Pauk et al (1991). In their report 6 parents and 10 F2-populations were tested on two media and the correlations between anther culture response and callus induction frequency were r = 0.92 and r = 0.91 for the two media, respectively.…”

Effect of copper on shoot and root regeneration in wheat, triticale, rape and tobacco tissue cultures

“…For induction of androgenic calli, C-17 medium (Huijun X. in Pauk et al 1991) was used with some modification (replacement of C-17 microelements with those of MS). Pretreatment of tillers, isolation of anthers and the culture conditions were similar to those described by Pauk et al (1991).…”

“…For induction of androgenic calli, C-17 medium (Huijun X. in Pauk et al 1991) was used with some modification (replacement of C-17 microelements with those of MS). Pretreatment of tillers, isolation of anthers and the culture conditions were similar to those described by Pauk et al (1991). Calli l mm in diameter were harvested and transferred to ½ strength MS medium with or without hormones (0.5+ 0.5 mg 1-1 NAA and KN) and containing different concentrations of CuSO 4 (0, 0.1 and 2 IxM).…”

The origin of clones among androgenic regenerants of hexaploid triticale

“…For the solid culture approach, modified 190-2 induction medium (Zhuang and Xu 1983) supplemented with 90 g/l maltose, 438 mg/l glutamine, 2 mg/l 2.4-D and 0.5 mg/l kinetin was used; liquid medium was the same but without growth regulators. Individual androgenic structures were manually selected under a dissecting microscope at their early stages of development and at the appropriate stage, transferred onto the solidified 190-2 regeneration medium (Zhuang and Xu 1983) supplemented with growth regulators according to Pauk et al (1991) and sub-cultured every 2 weeks. The cultures were kept under a 16/8-h (day/night) photoperiod.…”