Androgenesis in rainbow trout using cryopreserved spermatozoa: the effect of processing and biological factors

Babiak, Igor; Dobosz, Stefan; Goryczko, K.; Kuźmiński, H.; Brzuzan, Paweł; Ciesielski, Sławomir

doi:10.1016/s0093-691x(02)00631-3

Cited by 65 publications

(71 citation statements)

References 33 publications

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“…Androgenesis Androgenetic development was induced in rainbow trout as described by Babiak et al (2002a). Gametes were collected from donors kept at the Department of Salmonid Research, Inland Fisheries Institute in Olsztyn, Rutki, Poland.…”

Section: Methodsmentioning

confidence: 99%

“…Gametes were collected from donors kept at the Department of Salmonid Research, Inland Fisheries Institute in Olsztyn, Rutki, Poland. Oocytes originated from six silver-blue-coloured females of the Rutki strain, whereas spermatozoa were obtained from a single yellow-coloured male from a gynogenetic yellow strain (Babiak et al, 2002a). Yellow colouration is recessive to wild colouration, therefore it can serve as a colour marker for efficiency of androgenesis: androgenetic progenies are yellowcoloured whereas control half-siblings are wild-coloured.…”

Section: Methodsmentioning

confidence: 99%

“…Androgenetic progenies were yellow-coloured and also homozygous at locus str60, a microsatellite marker (Estoup et al, 1993) used to prove the occurrence of androgenetic development. Control half-siblings were wild-coloured, and the founder father was heterozygous at the locus str60 (Babiak et al, 2002a) until their maturity. Karyology was conducted on the material derived from 16 adult, healthy, morphologically normal 3-year-old individuals.…”

Section: Methodsmentioning

confidence: 99%

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The stability of telomereless chromosome fragments in adult androgenetic rainbow trout

Ocalewicz

Babiak

Dobosz

et al. 2004

Journal of Experimental Biology

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SUMMARY The study provides new data on the stability of gamma radiation-induced chromosome fragments of a putative maternal nuclear genome in an androgenetic vertebrate, rainbow trout (Oncorhynchus mykiss Walbaum). The fragments were found in five of 16 examined individuals and they were mostly centromeric parts of metacentric or subtelocentric chromosomes. Chromosome fragments were identical in all cells of a given androgenetic individual,indicating that segregation of chromosome fragments is active from the early cell divisions. Most of the fragments were telomereless, i.e. they had no telomeric sequences on their ends. This shows that telomeres are not necessary for stability of chromosomal structures in a vertebrate genome. In one individual, the interstitial telomeric sites were found in chromosomes, which could be the effect of joining chromosome fragments.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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The stability of telomereless chromosome fragments in adult androgenetic rainbow trout

Ocalewicz

Babiak

Dobosz

et al. 2004

Journal of Experimental Biology

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“…The artificial induction of androgenesis fish has primarily been attempted in O. mykiss, Platichthys flesus, Salvelinus japonicus, Oreochromis niloticus, Danio rerio, Oncorhynchus masou, Ctenopharyngodon idellu, Esox reticulatu, Misgurnus anguillicaudrus, Pelteobagrus fulvidraco, and so on [92,[109][110][111]. It is worth noting that because male tilapia grows more rapidly than female tilapia, androgenesis could be used to rapidly obtain super-male individuals with a male nucleus (YY).…”

Section: Artificial Gynogenesis and Androgenesismentioning

confidence: 99%

Development and application of biological technologies in fish genetic breeding

Duan

Xiao

et al. 2015

Sci. China Life Sci.

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Fish genetic breeding is a process that remolds heritable traits to obtain neotype and improved varieties. For the purpose of genetic improvement, researchers can select for desirable genetic traits, integrate a suite of traits from different donors, or alter the innate genetic traits of a species. These improved varieties have, in many cases, facilitated the development of the aquaculture industry by lowering costs and increasing both quality and yield. In this review, we present the pertinent literatures and summarize the biological bases and application of selection breeding technologies (containing traditional selective breeding, molecular marker-assisted breeding, genome-wide selective breeding and breeding by controlling single-sex groups), integration breeding technologies (containing cross breeding, nuclear transplantation, germline stem cells and germ cells transplantation, artificial gynogenesis, artificial androgenesis and polyploid breeding) and modification breeding technologies (represented by transgenic breeding) in fish genetic breeding. Additionally, we discuss the progress our laboratory has made in the field of chromosomal ploidy breeding of fish, including distant hybridization, gynogenesis, and androgenesis. Finally, we systematically summarize the research status and known problems associated with each technology. fish genetic breeding, genetic improvement, biological method, traits, new variety Citation:

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“…Although androgenesis performed by using frozen sperm and γ-ray-inactivated xenogenic eggs can regenerate live fish, their survival rate is extremely low, and resulting offspring become nuclear-cytoplasmic hybrids (11). The loss of maternally inherited materials including mitochondrial DNA makes this method impractical, as it also does for the transfer of nuclei from cryopreserved somatic cells into xenogenic oocytes (12,13).…”

mentioning

confidence: 99%

Generation of functional eggs and sperm from cryopreserved whole testes

Lee

Iwasaki

Shikina

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

133

118

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The conservation of endangered fish is of critical importance. Cryobanking could provide an effective backup measure for use in conjunction with the conservation of natural populations; however, methodology for cryopreservation of fish eggs and embryos has not yet been developed. The present study established a methodology capable of deriving functional eggs and sperm from frozen type A spermatogonia (ASGs). Whole testes taken from rainbow trout were slowly frozen in a cryomedium, and the viability of ASGs within these testes did not decrease over a 728-d freezing period. Frozenthawed ASGs that were intraperitoneally transplanted into sterile triploid hatchlings migrated toward, and were incorporated into, recipient genital ridges. Transplantability of ASGs did not decrease after as much as 939 d of cryopreservation. Nearly half of triploid recipients produced functional eggs or sperm derived from the frozen ASGs and displayed high fecundity. Fertilization of resultant gametes resulted in the successful production of normal, frozen ASG-derived offspring. Feasibility and simplicity of this methodology will call for an immediate application for real conservation of endangered wild salmonids.fish genetic resources | testes cryopreservation | germ cell transplantation | stem cell | triploid trout B ecause of the environmental effects of human activities, a growing number of fish species have become threatened or are already extinct (1). Salmonid species are particularly vulnerable to habitat destruction and the effects of climate change as they occupy a unique ecological niche resulting from their longrange migration between freshwater and marine habitats (2, 3). Conservation strategies for these threatened fish must therefore be developed and implemented with all possible haste. Complicating the conservation of threatened salmonid populations are the risks associated with captive breeding and translocation of live fish, such as facility accidents, pathogen infections, genetic drift, and reduced fitness of individuals within natural habitats (4, 5).Cryobanking of fish gametes to semipermanently (6) store genetic resources could serve as an alternative approach to traditional conservation methods (7); however, past attempts at cryopreservation of fish embryos and mature oocytes have been unsuccessful (8), as a result of their large size, high yolk content, and low membrane permeability (9, 10). Although androgenesis performed by using frozen sperm and γ-ray-inactivated xenogenic eggs can regenerate live fish, their survival rate is extremely low, and resulting offspring become nuclear-cytoplasmic hybrids (11). The loss of maternally inherited materials including mitochondrial DNA makes this method impractical, as it also does for the transfer of nuclei from cryopreserved somatic cells into xenogenic oocytes (12, 13). In zebrafish, in vitro maturation of immature oocytes subsequent to cryopreservation is also impossible. Although techniques for cryopreservation of stage I and II oocytes (diameter, 90-350 μm) (14) and ...

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Androgenesis in rainbow trout using cryopreserved spermatozoa: the effect of processing and biological factors

Cited by 65 publications

References 33 publications

The stability of telomereless chromosome fragments in adult androgenetic rainbow trout

The stability of telomereless chromosome fragments in adult androgenetic rainbow trout

Development and application of biological technologies in fish genetic breeding

Generation of functional eggs and sperm from cryopreserved whole testes

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