Androgenesis of highly recalcitrant rice genotypes with maltose and silver nitrate

Lentini, Zaida; Reyes, Patricia; Martínez, César; Roca, William M.

doi:10.1016/0168-9452(95)04180-3

Cited by 72 publications

(63 citation statements)

References 21 publications

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“…The use of sucrose might promotes in vitro production of ethylene in excised tissues causing the browning of callus, the substitution of maltose for sucrose could help to protect the calli by reducing the production of ethylene. Similar results that maltose is better sources of carbohydrate in both the subculture and differentiation media were obtained by Lin et al [9].…”

Section: Influence Of Maltose On Browning Secretion In Zygotic Embryosupporting

confidence: 77%

Regeneration of <i>in Vitro</i> Shoot and Root Structure through Hormone Manipulation of Coconut (MATAG F2) Zygotic Embryos

Zuraida¹,

Sentoor²,

Ahmad³

et al. 2017

AJPS

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Zygotic embryos tissues have found to be more responsive explants for clonal propagation of coconut. In the present study, the feasibility of using zygotic embryos explants for clonal propagation of a local coconut variety MATAG F2 was assessed. Callus was induced by incorporating of cytokinin and auxin into the medium. The sliced embryos explants were immersed in 1 M maltose for 60 mins, then with 0.05 M maltose for 1 min and followed by 0.01 M maltose for 5 mins was the best for prevention of phenolic compounds excretion. The callus formation depended on the concentration of 2,4-D in the media and the best effect was observed with the high level (2,4-D and BAP) tested. Attempts at inducing multiple shoot from the zygotic embryos callus were unsuccessful. No multiple shoots was present; most of the callus became root structure.

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Section: Influence Of Maltose On Browning Secretion In Zygotic Embryosupporting

confidence: 77%

Regeneration of <i>in Vitro</i> Shoot and Root Structure through Hormone Manipulation of Coconut (MATAG F2) Zygotic Embryos

Zuraida¹,

Sentoor²,

Ahmad³

et al. 2017

AJPS

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“…According to Lentini et al [55], maltose has been described as an agent that may regulate osmotic potential of cellular environment of callus. Since sucrose promotes in vitro production of ethylene in excised tissues causing the browning of callus, the substitution of maltose for sucrose may help to protect the calli by reducing the production of ethylene.…”

Section: Resultsmentioning

confidence: 99%

High Frequency Embryogenic Callus Induction and Whole Plant Regeneration in Japonica Rice Cv. Kitaake

Sah¹,

Kaur²

2014

J Rice Res

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A new and rapid protocol for production of embryogenic callus and plant regeneration has been reported in Oryza sativa cv. Kitaake. Callus cultures were established from seeds of Kitaake on MS medium supplemented with 2,4-D (3.0 mg/l), BAP (0.25 mg/l) and proline (0.6 g/l) gelled with phytagel (3.0 g/l), after 9 days of seed culturing. The embryogenic calli were regenerated on regeneration medium, i.e. MS medium supplemented with BAP (3.0 mg/l) and NAA (0.2 mg/l) and gelled with agar (8.0 g/l) in combination with phytagel (2.0 g/l) and regeneration occurred in 18 days. The regeneration percentage achieved was very high i.e 82.66% on the above medium. Rooting was achieved on half strength MS medium. The plantlets were hardened and transferred to soil in earthen pots. The regeneration protocol so developed through callus formation was highly reproducible. The plants showed normal growth and flowering under glass house conditions as well as in field conditions.

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“…This might be due to the lower level of nitrogen in N6 as compared to MS and SK1. Lentini et al (1995) demonstrated the beneficial effects of lowered NH4+ levels on callus induction in indica rice. In addition to basal media, the plant growth regulators are also equally important in calli induction.…”

Section: Discussionmentioning

confidence: 99%

Doubled Haploids generated through anther culture from an elite long duration rice hybrid, CRHR32: Method optimization and molecular characterization

Rout

Naik

Ngangkham

et al. 2016

Plant Biotechnology

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An improved procedure has been developed for high frequency androgenesis in an elite long duration indica rice hybrid. The effects of cold temperature pretreatment, duration of treatment and media with different plant growth regulators on callus induction and shoot regeneration were examined for generation of doubled haploids. N6 medium supplemented with 2.0 mg l −1 2,4-D, 0.5 mg l −1 BAP and 30 g l −1 maltose was found to be most effective for callusing when compared with MS and SK1. The N6 media grown calli showed maximum green shoot regeneration frequency in MS medium supplemented with 0.5 mg l −1 NAA, 0.5 mg l −1 Kinetin, 1.5 mg l −1 BAP and 30 g l −1 sucrose after 2 week of culture. The cold temperature treatment of spike at 10°C for 2 days alongside by 8 days was found to be most suitable conditions for callusing and green shoot regeneration producing 186 green plants from indica rice hybrid, CRHR32. The ploidy status assessed on the basis of morpho-agronomic characters revealed fertile diploids at a frequency of about 81.10%; 16.10%, 2.68% and 1.08% were polyploids, haploids and mixploids respectively. Microsatellite marker analysis showed 1 : 1 ratio of the alleles of CMS and restorer lines used for development of CRHR32. Homozygosity was detected for all the marker loci in 150 DHs and only one plant was identified as heterozygote. This investigation identified the favorable media composition and condition for callus induction and green plant regeneration which would further increase the knowledge and better understanding in rice hybrids for development of DHs.

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Androgenesis of highly recalcitrant rice genotypes with maltose and silver nitrate

Cited by 72 publications

References 21 publications

Regeneration of <i>in Vitro</i> Shoot and Root Structure through Hormone Manipulation of Coconut (MATAG F2) Zygotic Embryos

Regeneration of <i>in Vitro</i> Shoot and Root Structure through Hormone Manipulation of Coconut (MATAG F2) Zygotic Embryos

High Frequency Embryogenic Callus Induction and Whole Plant Regeneration in Japonica Rice Cv. Kitaake

Doubled Haploids generated through anther culture from an elite long duration rice hybrid, CRHR32: Method optimization and molecular characterization

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Androgenesis of highly recalcitrant rice genotypes with maltose and silver nitrate

Cited by 72 publications

References 21 publications

Regeneration of &lt;i&gt;in Vitro&lt;/i&gt; Shoot and Root Structure through Hormone Manipulation of Coconut (MATAG F2) Zygotic Embryos

Regeneration of &lt;i&gt;in Vitro&lt;/i&gt; Shoot and Root Structure through Hormone Manipulation of Coconut (MATAG F2) Zygotic Embryos

High Frequency Embryogenic Callus Induction and Whole Plant Regeneration in Japonica Rice Cv. Kitaake

Doubled Haploids generated through anther culture from an elite long duration rice hybrid, CRHR32: Method optimization and molecular characterization

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Regeneration of <i>in Vitro</i> Shoot and Root Structure through Hormone Manipulation of Coconut (MATAG F2) Zygotic Embryos

Regeneration of <i>in Vitro</i> Shoot and Root Structure through Hormone Manipulation of Coconut (MATAG F2) Zygotic Embryos