Androgenic activation, impairment of the monoaminergic system and altered behavior in zebrafish larvae exposed to environmental concentrations of fenitrothion

Faria, Melissa; Prats, Eva; Ramírez, Jonathan Ricardo Rosas; Bellot, Marina; Bedrossiantz, Juliette; Pagano, Maria; Valls, Arnau; Gómez‐Canela, Cristian; Porta, Josep M.; Mestres, Jordi; García-Reyero, Natàlia; Faggio, Caterina; Gómez-Oliván, Leobardo Manuel; Raldúa, Demetrio

doi:10.1016/j.scitotenv.2021.145671

Cited by 55 publications

(19 citation statements)

References 77 publications

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“…A total inhibition of zebrafish MAO activity has been found in larvae exposed to 5 µM deprenyl for only 24 h. As expected, fluoxetine, a chemical targeting SERT activity, did not affect the activity of this enzyme. A similar inhibition of MAO activity by deprenyl was recently reported in zebrafish larvae [27]. Furthermore, Sallinen at al.…”

Section: Increase Of Serotonin Signalingsupporting

confidence: 81%

Pharmacological Modulation of Serotonin Levels in Zebrafish Larvae: Lessons for Identifying Environmental Neurotoxicants Targeting the Serotonergic System

Faria

Prats

Bellot

et al. 2021

Toxics

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This study examines the effects of acute pharmacological modulation of the serotonergic system over zebrafish larvae’s cognitive, basic, and defense locomotor behaviors, using a medium to high throughput screening assay. Furthermore, the relationship between behavior, enzyme activity related to neurotransmitter metabolism, neurotransmitter levels, and gene expression was also determined. Modulation of larvae serotonergic system was accomplished by 24 h exposure to single and opposite pharmacodynamics co-exposure to three model psychopharmaceuticals with antagonistic and agonistic serotonin signaling properties: 2.5 mM 4-Chloro-DL-phenylalanine (PCPA) and 5 µM deprenyl and 0.5 µM fluoxetine, respectively. Similar behavioral outcome was observed for deprenyl and fluoxetine, which was reflected as hypolocomotion, decrease in larvae defensive responses, and cognitive impairment. Contrarily, PCPA induced hyperlocomotion and increase in larvae escape response. Deprenyl exposure effects were more pronounced at a lower level of organization than fluoxetine, with complete inhibition of monoamine oxidase (MAO) activity, dramatic increase of 5-HT and dopamine (DA) levels, and downregulation of serotonin synthesis and transporter genes. PCPA showed mainly effects over serotonin and dopamine’s main degradation metabolites. Finally, co-exposure between agonistic and antagonist serotonin signaling drugs reviled full recovery of zebrafish impaired locomotor and defense responses, 5-HT synthesis gene expression, and partial recovery of 5-HT levels. The findings of this study suggest that zebrafish larvae can be highly sensitive and a useful vertebrate model for short-term exposure to serotonin signaling changes.

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Section: Increase Of Serotonin Signalingsupporting

confidence: 81%

Pharmacological Modulation of Serotonin Levels in Zebrafish Larvae: Lessons for Identifying Environmental Neurotoxicants Targeting the Serotonergic System

Faria

Prats

Bellot

et al. 2021

Toxics

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“…In addition, because the embryos and early adults are optically transparent, a direct observation of internal organs and morphological characteristics by light or fluorescence microscopy is possible [ 13 , 14 , 15 , 16 ]. Furthermore, the facilities required to house zebrafish for scientific purposes are relatively simple and cheaper, and the experiments are performed across different developmental stages (e.g., embryo, larva or adult) and in a wide variety of laboratory conditions (e.g., large number of compounds from diverse classes in parallel, acute and chronic testing and a wide range of concentrations of the chemicals tested) [ 17 , 18 , 19 , 20 , 21 ]. Generally, the zebrafish acute and chronic toxicity tests are performed according to the guideline 203 of OECD (updated 2019) and guideline 230 of OECD (2009) [ 22 , 23 , 24 , 25 , 26 ], while guideline 236 of OECD (2013) is used to determine the acute toxicity of chemicals on embryonic stages [ 27 , 28 , 29 ].…”

Section: Introductionmentioning

confidence: 99%

Zebrafish as a Screening Model to Study the Single and Joint Effects of Antibiotics

et al. 2021

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The overuse of antibiotics combined with the limitation of wastewater facilities has resulted in drug residue accumulation in the natural environment. Thus, in recent years, the presence of antibiotic residues in the environment has raised concerns over the potential harmful effects on ecosystems and human health. The in vivo studies represent an essential step to study the potential impact induced by pharmaceutical exposure. Due to the limitations of traditional vertebrate model systems, zebrafish (Danio rerio) has recently emerged as a promising animal model to study the toxic effects of drugs and their therapeutic efficacy. The present review summarizes the recent advances made on the toxicity of seven representative classes of antibiotics, namely aminoglycosides, β-lactams, macrolides, quinolones, sulfonamides, tetracyclines and polyether antibiotics, in zebrafish, as well as the combined effects of antibiotic mixtures, to date. Despite a significant amount of the literature describing the impact of single antibiotic exposure, little information exists on the effects of antibiotic mixtures using zebrafish as an animal model. Most of the research papers on this topic have focused on antibiotic toxicity in zebrafish across different developmental stages rather than on their efficacy assessment.

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“…Due to the extensive use of PCPs (e.g., sunscreens, lotions, shampoos, decorative cosmetics) with UVs, these substances have become a significant micro-pollutant of our environment and extensive scientific research has been performed to investigate their toxicological effect on non-target organisms (e.g., fish, corals, mussels) [37]. Aquatic organisms are exposed to various of micropollutants from their early life stage to their adulthood [38][39][40][41][42][43][44]. It has been revealed that UVs are able to bioaccumulate in the organism and biomagnificate through the food chain [3,43,45].…”

Section: Discussionmentioning

confidence: 99%

Do Single-Component and Mixtures Selected Organic UV Filters Induce Embryotoxic Effects in Zebrafish (Danio rerio)?

Cahova

Blahová

Plhalová

et al. 2021

Water

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UVs are important ingredients in common cosmetic products (e.g., sunscreens, hairsprays, soap). After their use, they can enter the aquatic ecosystem and negatively affect non-target aquatic organisms. The aim of our study was to evaluate acute embryotoxicity of selected organic UVs 2-phenylbenzimidazole-5-sulfonic acid (PBSA), ethylhexyl methoxycinnamate (EHMC), octocrylene (OC), 4-methylbenzylidene camphor (4-MBC) and benzophenone-3 (BP-3). The chemicals were tested both as a single substance and their mixtures. The types of mixtures were chosen as follows: the combination of OC and 4-MBC; the combination of PBSA, EHMC and BP-3 and the combination of all five UV filters. The embryotoxicity was evaluated using a modified method of the Fish Embryo Acute Toxicity Test-OECD guideline 236 and zebrafish (Danio rerio) was selected as a suitable fish model organism. The toxic effects were studied by assessing mortality, hatching and the occurrence of malformations at 24, 48, 72 and 96 h post fertilization. The obtained results indicate that especially the mixture of OC and 4-MBC presents a potential risk of embryotoxicity for zebrafish due to a significant increase in mortality, which was 41.7% in the experimental group exposed to 10 μg/L at 96 h post fertilization. Based on our results, the most effected sub-lethal endpoints were hatching and malformation (e.g., edema of pericard, bent spine, yolk edema), but with no statistically significant effect. These results differ within groups with single UVs and with their mixtures, suggesting the interaction of these substances when they are exposed together.

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Androgenic activation, impairment of the monoaminergic system and altered behavior in zebrafish larvae exposed to environmental concentrations of fenitrothion

Cited by 55 publications

References 77 publications

Pharmacological Modulation of Serotonin Levels in Zebrafish Larvae: Lessons for Identifying Environmental Neurotoxicants Targeting the Serotonergic System

Pharmacological Modulation of Serotonin Levels in Zebrafish Larvae: Lessons for Identifying Environmental Neurotoxicants Targeting the Serotonergic System

Zebrafish as a Screening Model to Study the Single and Joint Effects of Antibiotics

Do Single-Component and Mixtures Selected Organic UV Filters Induce Embryotoxic Effects in Zebrafish (Danio rerio)?

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