Androgenic response of Brazilian wheat genotypes to different pretreatments of spikes and to a gelling agent

Poersch-Bortolon, Liane Balvedi; Scagliusi, S. M. M.; Lau, Elene Yamazaki; Bodanese‐Zanettini, Maria Helena

doi:10.1590/s0100-204x2016001100006

Cited by 3 publications

(2 citation statements)

References 29 publications

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“…Finally, we also highlighted a novel reactivity marker using the viable microspores detected in the P3 polygon by IFC technology to predict embryo yields in Pavon microspore suspensions. With an average of 13.5 embryos per 10,000 microspores, Pavon's embryo yields in our study fit with the other yields published for this genotype, which corroborate the reactivity threshold we set up [62,63]. To our knowledge, this is the first time that embryo yields have been predicted early and reliably in androgenesis.…”

Section: Discussionsupporting

confidence: 87%

Impedance flow cytometry allows the early prediction of embryo yields in wheat (Triticum aestivum L.) microspore cultures

Canonge¹,

Philippot²,

Leblanc

et al. 2020

Plant Science

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Haplomethods are key biotechnological tools that make it possible to rapidly produce perfectly homozygous lines, speeding up plant breeding programs. Under specific stress conditions, microspores are reprogrammed toward sporophytic pathways, leading to embryo formation.Various endogenous and exogenous factors affect embryo yield in androgenesis, so the improvement of androgenesis efficiency requires the development of early, reliable and robust reactivity markers. During the last decade, numerous cytological, cellular and biochemical approaches were carried out to finely characterize microspore development and fate during androgenesis. However, the different available markers are often species-dependent, and their development and application are time-consuming and cumbersome. In this study, we show the suitable use of impedance flow cytometry (IFC) to develop new robust, reliable and strong markers of androgenesis reactivity in wheat, leading to: (i) routine monitoring of the viability of heterogeneous cell cultures; (ii) quick and simple evaluation of stress treatment efficiency; and (iii) early prediction of embryo yields from microspore suspensions. IFC can therefore provide the fine characterization of all of the microspore developmental pathways that occur in a cell suspension, for embryogenic microspores as well as pollen-like microspores. IFC technology has become a very useful tool to track and characterize wheat microspores in androgenesis, but can also be adapted to other species and other in vitro cell culture systems.

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Section: Discussionsupporting

confidence: 87%

Impedance flow cytometry allows the early prediction of embryo yields in wheat (Triticum aestivum L.) microspore cultures

Canonge¹,

Philippot²,

Leblanc

et al. 2020

Plant Science

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show abstract

“…Three wheat (Triticum aestivum L.) genotypes (Brilhante, PF37 and PF62) were used in this study (Poersch-Bortolon et al, 2016). Fifty seeds per meter in 15 cm row distance (five rows per container) were sown on May 12, 2016 into six containers (two containers per genotype) in a randomized block design.…”

Section: Wheatmentioning

confidence: 99%

Genotype Specific Photosynthesis x Environment Interactions Captured by Automated Fluorescence Canopy Scans Over Two Fluctuating Growing Seasons

et al. 2019

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Photosynthesis reacts dynamic and in different time scales to changing conditions. Light and temperature acclimation balance photosynthetic processes in a complex interplay with the fluctuating environment. However, due to limitations in the measurements techniques, these acclimations are often described under steady-state conditions leading to inaccurate photosynthesis estimates in the field. Here we analyze the photosynthetic interaction with the fluctuating environment and canopy architecture over two seasons using a fully automated phenotyping system. We acquired over 700,000 chlorophyll fluorescence transients and spectral measurements under semi-field conditions in four crop species including 28 genotypes. As expected, the quantum efficiency of the photosystem II (F v /F m in the dark and F q '/F m ' in the light) was determined by light intensity. It was further significantly affected by spectral indices representing canopy structure effects. In contrast, a newly established parameter, monitoring the efficiency of electron transport (F r2 /F v in the dark respective F r2 '/F q ' in the light), was highly responsive to temperature (R 2 up to 0.75). This parameter decreased with temperature and enabled the detection of cold tolerant species and genotypes. We demonstrated the ability to capture and model the dynamic photosynthesis response to the environment over entire growth seasons. The improved linkage of photosynthetic performance to canopy structure, temperature and cold tolerance offers great potential for plant breeding and crop growth modeling.

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Impact of Ionic Liquids on Induction of Wheat Microspore Embryogenesis and Plant Regeneration

et al. 2020

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Ionic liquids are novel compounds with unique chemical and physical properties. They can be received based on synthetic auxins like 2,4-dichlorophenoxyacetic acid or dicamba, which are commonly used hormones in microspore embryogenesis. Nevertheless, ionic liquids have not been adapted in plant in vitro culture thus far. Therefore, we studied the impact of ionic liquids on the ability to undergo microspore embryogenesis in anther cultures of wheat. Two embryogenic and two recalcitrant genotypes were used for this study. Ten combinations of ionic liquids and 2,4-dichlorophenoxyacetic acid were added to the induction medium. In most cases, they stimulated induction of microspore embryogenesis and green plant regeneration more than a control medium supplemented with only 2,4-dichlorophenoxyacetic acid. Two treatments were the most favorable, resulting in over two times greater efficiency of microspore embryogenesis induction in comparison to the control. The effect of breaking down the genotype recalcitrance (manifested by green plant formation) was observed under the influence of 5 ionic liquids treatments. Summing up, ionic liquids had a positive impact on microspore embryogenesis induction and green plant regeneration, increasing the efficiency of these phenomena in both embryogenic and recalcitrant genotypes. Herbicidal ionic liquids can be successfully used in in vitro cultures.

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Androgenic response of Brazilian wheat genotypes to different pretreatments of spikes and to a gelling agent

Cited by 3 publications

References 29 publications

Impedance flow cytometry allows the early prediction of embryo yields in wheat (Triticum aestivum L.) microspore cultures

Impedance flow cytometry allows the early prediction of embryo yields in wheat (Triticum aestivum L.) microspore cultures

Genotype Specific Photosynthesis x Environment Interactions Captured by Automated Fluorescence Canopy Scans Over Two Fluctuating Growing Seasons

Impact of Ionic Liquids on Induction of Wheat Microspore Embryogenesis and Plant Regeneration

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