Andrographis paniculata (Burm.f.) Nees Alleviates Methotrexate-Induced Hepatotoxicity in Wistar Albino Rats

Abstract

Andrographis paniculata is a herbal plant used in traditional medicinal approaches to treat various ailments and diseases. Methotrexate (MTX) is a clinically used immunosuppressant and anticancer drug. One of the increasing concerns with MTX use is liver toxicity. The aim of this study is to investigate the potential effect of aqueous leaf extract of Andrographis paniculata against methotrexate-induced hepatotoxicity. Wistar albino rats were grouped into five groups, and the drugs were administered. MTX (20 mg/kg b.w.) was intraperitoneally injected into rats on the ninth day alone. Aqueous leaf extract of Andrographis paniculata (500 mg/kg b.w./day) was orally administered for 10 days. We confirmed the beneficial effect of aqueous extracts of Andrographis paniculata on restoring the hepatic enzyme markers, lipid profile, antioxidant level, anti-inflammatory marker (IL-10), anti-apoptosis (bcl-2), significant suppression of inflammatory cytokines (TNF-α, and IL-6), apoptosis marker (caspase 3) and cellular tissue damage caused by MTX. Overall, we revealed that Andrographis paniculata reduces critical aspects of oxidative stress, inflammatory processes, and apoptosis, thus protecting against methotrexate-induced hepatotoxicity.

Potential Hepatoprotective Effects of Chamaecyparis lawsoniana against Methotrexate-Induced Liver Injury: Integrated Phytochemical Profiling, Target Network Analysis, and Experimental Validation

“…According to MS mass, MS 2 fragmentation data and patterns, and literature reports, 61 chemical constituents were identified, categorized into flavonoids and glycosides, phenolic, diterpene, carboxylic, sugar acids, fatty acids, lignans, and other compounds. Retention time, pseuomolecular ion peak [M-H] − , MS 2 , and the related literature of the identified metabolites of CLAE are listed in Table 1. Figure S1 shows the total ion chromatogram (TIC) of CLAE in negative mode.…”

“…One of the common problems associated with liver function is drug-induced liver injury (DILI), which refers to the side effects caused by medications and is often the leading cause of acute liver failure. This condition can not only impede therapeutic progress but also restrict drug development and result in the discontinuation of specific medications from the market [1,2]. Methotrexate (MTX), also known as amethopterin, is a versatile medication that has been proven effective in treating a wide range of medical conditions.…”