Anesthesia and recovery with tricaine methanesulfonate, eugenol and thiopental sodium in the carp, Cyprinus carpio.

Hikasa, Yoshiaki; Takase, Katsuaki; Ogasawara, Toshimi; Ogasawara, Satoshi

doi:10.1292/jvms1939.48.341

Cited by 133 publications

(133 citation statements)

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“…the flounder P. flesus (Akbulut et al, 2012b), the Russian sturgeon A. gueldenstaedtii (Akbulut et al, 2011b), the Persian sturgeon A. persicus (Imanpoor et al, 2010) and the Siberian sturgeon A. baerii (Akbulut et al, 2012a), which ranged between 400 and 750 mg L -1 . In general, highwater temperatures enhance anaesthetic efficacy of clove oil by shortening induction time in the steelhead fry O. mykiss (Woolsey et al, 2004), the gilthead sea bream Sparus aurata (Mylonas et al, 2005), the common carp C. carpio (Hikasa et al, 1986) and the flounder, P. flesus (Akbulut et al,2012b). Hamackova et al (2006), studied with perch (Perca fluviatilis), reported full anesthesia after 7.57 min at 12.5°C while 6.98 min at 17.5°C, and/or 6.05 min at 12.5°C and 3.73 min at 20.0°C after the exposure to clove oil.…”

Section: Discussionmentioning

confidence: 99%

“…Eugenol has a multitude of properties making it useful in a wide variety of applications, including as an antioxidant (Kramer, 1985;Pulla and Lokesh, 1992), antifungal (Kamble and Patil, 2008;Hoskonen et al 2015), antibacterial (Karapmar and Aktug,1987;Kouidhi et al, 2010) or as an antiparasitic agent (Machado et al, 2011). Several studies have shown that clove oil is an effective agent in the sedation of larvae (Akbulut et al, 2011a), fry (Endo et al, 1972;Woolsey et al, 2004;Akbulut et al, 2012a), juvenile (Keene et al, 1998;Uçar and Atamanalp, 2010;Akbulut et al, 2011b) and adult fish of various species (Hikasa et al, 1986;Wagner et al, 2002;Hoskonen and Pirhonen, 2004). Among flatfish species, clove oil efficacy has been tested on the sole Solea senegalensis (Weber et al, 2009) and on the flounder Platichthys flesus (Akbulut et al, 2012b), but it has never been tested on the anaesthesia of turbot Psetta maximawith relation to temperature and fish size.…”

Section: öZetmentioning

confidence: 99%

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Untitled

Aydın¹,

Akbulut²,

Küçük³

et al. 2015

Turk. J. Fish. Aquat. Sci.

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The effects of four concentrations of clove oil (150, 300, 450 and 600 mg L -1 ) as ananaesthetic substance at three temperature levels (10, 15 and 20°C) and three fish size (as weight) groups (57.0±11.3, 103.0±11.9 and 457.0±89.8 g) on induction and recovery times in turbot (Psetta maxima) were investigated in this study. The fish were individually exposed to each clove oil bath in 10, 20 or 50L buckets with respect to fish size and were recovered in a 500-L tank with running seawater. Temperature, fish size and clove oil concentrations were found to have significant effects on induction and recovery times in turbot (P<0.01). Induction and recovery times were both less than half when the temperature was increased from 10 to 20°C (P<0.01). The duration to reach surgical anaesthesia and recovery times of small size turbot varied in relation to temperature, but were generally shorter than big size turbot (P<0.01). Overall, the increase of clove oil concentration from 150 to 600 mg L -1 decreased the induction time by a factor of 2.3 but on the contrary, prolonged the full recovery time by a factor of 1.92. The surgical anesthesia was attained in all the groups under 4.45 min at 150 mg L -1 or even under 2 min at 600 mg L -1 clove oil concentration. However, the time of recovery ranged from 4.32 min (150 mg L -1 ) to 8.29 min (600 mg L -1 ). Based on our results, the clove oil concentrations around 180-220 mg L -1 appeared to be adequate to be used for fast anaesthesia and relatively short recovery time for turbot. Mean effective concentration (EC50) of clove oil was calculated as 190.00 ± 10.34 mg L -1 with 95% confidence limits for overall three temperature and fish sizes. This study has demonstrated that clove oil can be safely and effectively used in the anaesthesia of turbot.

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Section: Discussionmentioning

confidence: 99%

Section: öZetmentioning

confidence: 99%

Untitled

Aydın¹,

Akbulut²,

Küçük³

et al. 2015

Turk. J. Fish. Aquat. Sci.

View full text Add to dashboard Cite

show abstract

“…Behavioural changes in each fish were observed according to Keene et al (1998). Clinical signs as lack of response to external stimuli and total loss of muscle tone were used to characterize the anaesthetic stage IV (deep anaesthesia) (adapted from Hikasa et al, 1986).…”

Section: Experiments 1: Anesthetic Sprinkling Method Viability and Pamentioning

confidence: 99%

“…After achieving the anesthetic stage V (Hikasa et al, 1986), the fish were euthanized through medullar section and gills were removed and separated into Petri dishes. It was considered the gills on the left side with the anesthetic (LSWE), and the gills of the right side, without anesthetic (RSWOE) the control group.…”

Section: Experiments 1: Anesthetic Sprinkling Method Viability and Pamentioning

confidence: 99%

Anesthesia by sprinkling method in the gills of tambaqui Colossoma macropomum does not influence intensity and morphology of monogeneans

et al. 2016

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The present study evaluates the influence of anesthesia on the parasitic fauna of monogenea fish parasites, as its intensity and viability. Two experiments were conducted: Evaluation of an anesthetic method by sprinkling eugenol directly on gills and evaluation of monogenea motility and viability; Comparison of immersion and directly sprinkling on the gills with benzocaine and eugenol followed by evaluation on parasite intensity. The results suggest that the anesthetic sprinkling didn't interfere in the parasite motility, morphology and body surface integrity analyzed by fluorescence method. The monogenean intensity in the gills was lower in fish anesthetized by immersion method compared to the sprinkling method and the control group. This method of anesthesia can be used in parasitological studies.Keywords: parasitology, animal welfare, humane slaughter, histopathology. Anestesia por meio de aspersão nas brânquias de tambaquiColossoma maropomum não influencia a intensidade e a morfologia de monogeneas ResumoO presente estudo avalia a influência da anestesia sobre a fauna parasitária de monogeneas em peixes, sua intensidade e sua viabilidade. Dois experimentos foram realizados: Avaliação de um método anestésico por aspersão eugenol diretamente nas brânquias e avaliação da motilidade das monogeneas e sua viabilidade; e Comparação entre imersão e aspersão diretamente nas brânquias com benzocaína e eugenol, seguido de avaliação sobre a intensidade parasitária. Os resultados sugerem que a aspersão do anestésico não interferiu na motilidade, morfologia, superfície corporal e integridade do parasita, analisadas pelo método de fluorescência. A intensidade de monogenéticos nas brânquias foi menor nos peixes anestesiados pelo método de imersão em comparação com o método de aspersão e o grupo controle. O método de anestesia por aspersão nas brânquias pode ser utilizado em estudos parasitológicos.Palavras-chave: parasitologia, bem-estar animal, o abate humanitário, histopatologia.

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“…Des études, récentes pour la plupart, ont mis en évidence les pouvoirs anesthésiques de l'huile de clou de girofle sur les poissons (ENDO et al, 1972 ;HIKASA et al, 1986 ;SOTTO et BURHANUDDIN, 1995 ;MUNDAY et WILSON, 1997 ;PEAKE, 1998 ;WATERSTRAT, 1999). Des études ont été réalisées chez les salmonidés TAYLOR et ROBERTS, 1999), mais aucune, à notre connaissance, ne s'est intéressée aux effets de cet anesthésique sur les smolts de saumon atlantique (Salmo salar L.) qui, à ce stade de leur développement, subissent de profonds changements physiologiques, susceptibles de modifier les effets de l'anesthésique.…”

Section: Introductionunclassified