Objective: To analyze the risk factors of neonatal medical adhesive-related skin injury and put forward targeted preventive measures, so as to provide reference for the care and prevention of neonatal medical adhesive-related skin injuries. Methods: Using the convenience sampling method, 262 neonates admitted to the neonatal intensive care unit (NICU) of a tertiary general hospital in Wenzhou from April 2021 to May 2022 were selected as the study subjects. The incidence of medical adhesive-related skin injuries in these neonates was retrospectively analyzed. Results: Among the 262 children, 43 cases had skin injuries, with an incidence rate of 16.4%. Single factor analysis showed that the occurrence of medical adhesive-related skin injury was related to gestational age, weight, electrocardiogram (ECG) monitoring, venous access, ambient temperature, and mechanical ventilation (P < 0.05). Multivariate logistic regression showed that gestational age, ECG monitoring, and ambient temperature were independent risk factors of medical adhesive-related skin injury (OR values were 0.700, 0.431, and 6.365, respectively). Conclusion: The high incidence of neonatal medical adhesive-related skin injury may be caused by one or more factors. Clinical measures should be taken, such as selecting the appropriate type of adhesive according to gestational age and using skin-protecting membrane, minimizing ECG monitoring, etc., to prevent the occurrence of neonatal medical adhesive-related skin injury.