“…Efficacy of lignocaine (20 mg/mL) for post-operative pain relief following castration of cattle has been investigated in numerous studies with various findings [17,24,25,26,27,28,29,30,31,32]. However, there are fewer studies that have assessed the efficacy of lignocaine (20 mg/mL) specifically for intra-operative pain relief during castration of cattle [17,28,30,32,33,34,35] (Table 1). Of these studies, pain during castration has been assessed using electroencephalographic responses [33], ocular temperature responses [34], cardiovascular responses [33,34], testicle retraction responses [35] and behavioural responses [17,28,30,32] to the procedure.…”