Aneuploidy of stem cells isolated from human adipose tissue

Бочков, Н. П.; Nikitina, V. A.; Buyanovskaya, O. A.; Voronina, E. S.; Goldstein, D. V.; Кулешов, Н. П.; Rzhaninova, A. A.; Chaushev, I. N.

doi:10.1007/s10517-008-0293-1

Cited by 19 publications

(19 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These fi ndings, our previous results [1,2], and pub lished data [8,10] disagree with the opinion of some scientists on genetic stability of SC and safety of their use without cytogenetic testing. The data on different types of chromosome changeability in cultured MSC necessitate more profound studies of SC genetics and creation of a system of their quality control for prevention of untoward consequences of therapeutic use of these cells.…”

Section: Incidence Of Aneuploidy In Early Passage Culturescontrasting

confidence: 79%

Spontaneous Aneuploidy and Clone Formation in Adipose Tissue Stem Cells during Different Periods of Culturing

et al. 2009

Self Cite

View full text Add to dashboard Cite

Cytogenetic analysis of 13 mesenchymal stem cell cultures isolated from normal human adipose tissue was carried out at different stages of culturing. The incidence of chromosomes 6, 8, 11, and X aneuploidy and polyploidy was studied by fluorescent in situ hybridization. During the early passages, monosomal cells were more often detected than trisomal ones. A clone with chromosome 6 monosomy was detected in three cultures during late passages.

show abstract

Section: Incidence Of Aneuploidy In Early Passage Culturescontrasting

confidence: 79%

Spontaneous Aneuploidy and Clone Formation in Adipose Tissue Stem Cells during Different Periods of Culturing

et al. 2009

Self Cite

View full text Add to dashboard Cite

show abstract

“…Monosomic nuclei predominated over trisomic ones. These data are in line with our previous data on MSC isolated from the adipose tissue [1]. The incidence of the studied autosome aneuploidy did not change over the course of MSC culturing (p>0.05; Table 3).…”

Section: Resultssupporting

confidence: 92%

Study of Genetic Stability of Human Bone Marrow Multipotent Mesenchymal Stromal Cells

et al. 2011

Self Cite

View full text Add to dashboard Cite

Immunophenotype, proliferation rate, and genetic stability parameters of bone marrow multipotent mesenchymal stromal cells were studied. Despite the reduction of proliferative activity by passages 11-12, the cells retained the characteristic immunophenotype. The incidence of spontaneous aneuploidy for autosomes 6, 8, 11 and sex chromosomes was evaluated. Two cultures of mesenchymal stromal cells carrying aneuploid cell clones were detected: with chromosome 8 trisomy and X chromosome monosomy. The results indicate the possibility of genetic transformation and selection of mesenchymal stromal cells with abnormal karyotype during in vitro culturing.

show abstract

“…According to our data, abnormal clones were detected in about 30% adipose tissue MSC and in 11% bone marrow MSC cultures [1,2]. Initial aspirated bone mar row or adipose tissue can be the sources of ab normal clones.…”

Section: Resultsmentioning

confidence: 62%

“…The problem of evaluating clone formation in mo saic cultures, discussed previously [2], deserves special attention. The presence of a clone in a culture is confi rmed by the presence of a group of cells with the same chromosome abnormality, but the problemwhich, specifi cally, incidence of aneuploid cells in the culture implies that this culture should be classifi ed as mosaic culture with clones -deserves a special study.…”

Section: Resultsmentioning

confidence: 99%