Angelica Sinensis polysaccharide antagonizes 5-Fluorouracil-induced spleen injury and dysfunction by suppressing oxidative stress and apoptosis

Du, Kunhang; Wang, Lu; Wang, Ziling; Xiao, Hanxianzhi; Hou, Jiying; Hu, Ling; Fan, Ningke; Wang, Yaping

doi:10.1016/j.biopha.2023.114602

Cited by 7 publications

(3 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…TBARS is one of the most widely used assay for measuring lipid peroxidation as MDA is a better predictor of oxidative damage (Al-Asmari et al, 2016). In our current study, a significant increase in TBARS levels indicates that 5-FU causes oxidative stress and our results are consistent with previous findings (Du et al, 2023).…”

Section: Immunoexpression Of Nf-кb and Caspaes-3supporting

confidence: 92%

Ameliorative Effect of Naringenin in 5-Fluorouracil-Induced Hepatotoxicity: An Experimental Study on Albino Wistar Rats

Vemula,

Mylaram,

Yadala

et al. 2024

IJAR

View full text Add to dashboard Cite

Background: Hepatotoxicity is an adverse complication in 5-Fluorouracil cancer treatment. Inflammation, oxidative stress, apoptosis and DNA damage are essential in the pathogenesis of liver damage. The present study evaluated the ameliorative effect of Naringenin (NG) in 5-FU at two different intervals in hepatic damage. Methods: Total (n=48) Albino Wistar rats were divided into 4 groups (N=12), Control-normal saline (group-1) per oral, 5-FU (group-2) @ 20 mg/kg b.wt was injected intraperitoneal (IP) for first 5 days, NG (group-3) @100 mg/kg b. wt/day orally for 28 days. Another group-4 was concurrently treated with NG along with 5-FU for 28 days and sacrificed on the 14th and 28th day of the experiment. Result: On exposure to 5-FU, biochemical variation of liver enzymes (ALT, AST, ALP and TP) were significantly increased while NG treatment significantly reduced these biomarkers in group 2 on 14th day and 28th day of the experiment. Furthermore, NG treatment significantly reduced lipid peroxidation and increases the antioxidant profile. It also significantly reduced elevated concentrations of pro-inflammatory cytokines like tumour necrosis factor (TNF-α), interleukin -1β (IL-1β), interleukin-6 (IL-6) with increased concentrations of interleukin-10 (IL-10) along with reduced immunohistochemical expression of nuclear factor kappa light chain enhancer of activated B cells (NF-κB) and Caspases -3 (Cas-3) by NG. On histopathology, pathological lesions were observed to support biochemical variations in group-2 rats. In group-4 rats, NG ameliorates liver enzymes, inflammatory cytokines and oxidative indices through an anti-oxidant, anti-inflammatory and anti-apoptotic property.

show abstract

Section: Immunoexpression Of Nf-кb and Caspaes-3supporting

confidence: 92%

Ameliorative Effect of Naringenin in 5-Fluorouracil-Induced Hepatotoxicity: An Experimental Study on Albino Wistar Rats

Vemula,

Mylaram,

Yadala

et al. 2024

IJAR

View full text Add to dashboard Cite

show abstract

“…In this study, proin ammatory factor IL-1β signi cantly increased following the administration of 5-FU treatment. However, ASP alleviated IL-1β levels somehow, which may be the reason that ASP suppressing oxidative stress and apoptosis, antagonizing 5-FU-induced spleen injury and dysfunction [25]. Surprisingly, it was also found that concomitant with the proliferation of splenic macrophages and c-kit + erythroid progenitor cells, ASP promoted the genetic expression of glucose transporter1 (Glut1), rate-limiting enzymes of glycolysis such as hexokinase2 (Hk2) and pyruvate kinase M (PkM), and key glycolytic genes, including phosphoglycerate kinase (Pgk), lactate dehydrogenase A (LdhA), and pyruvate dehydrogenase kinase (Pdk-1), indicating ASP facilitates glycolysis of splenocytes.…”

Section: Discussionmentioning

confidence: 97%

Angelica sinensis polysaccharides promote extramedullary stress erythropoiesis via ameliorating splenic glycolysis and EPO/STAT5 signaling-regulated macrophages

Sun,

Wang,

Jiang

et al. 2024

Preprint

Self Cite

View full text Add to dashboard Cite

Background Extrinsic molecular mechanisms that regulate extramedullary stress erythropoiesis are still poorly understood, and the exploration of potential protective medication is needed. Materials and methods Peripheral blood parameters and BFU-E colony enumeration were measured. IHC staining was conducted to detect the proliferation of splenocytes and splenic F4/80 macrophages. The expression of β-catenin protein in RAW264.7 macrophages was assessed using immunofluorescence. The cell cycle of mouse spleen c-kit+ cells was analyzed by flow cytometry assay. Detection of Ccl2, Hk2, and Pgk1 mRNA expression by RT-qPCR. Cyclin D1 protein expression was assessed using Western blotting. IL-1 and EPO levels were determined by ELISA assay. Results In the 5-FU pre-administrated mouse, ASP rescued peripheral blood parameters such as RBC counts, HGB, HCT and MCH, and the BFU-E colony enumeration in the bone marrow. Meanwhile, ASP increased cellular proliferation in the splenic red pulp and cyclin D1 expression, ASP increased macrophage chemokine Ccl2 genetic expression and the number of F4/80 macrophages in the spleen and splenic BFU-E enumeration. Furthermore, ASP facilitated glycolytic genes including Hk2, Pgk1, Pkm, Pdk1 and Ldha via PI3K/Akt/HIF2α signaling in the spleen. Subsequently, ASP declined pro-proinflammatory factor IL-1β, whereas upregulating erythroid proliferation-associated genes Gdf15, Bmp4, Wnt2b, and Wnt8a. Moreover, ASP facilitated EPO/STAT5 signaling in splenic macrophages to enhance erythroid lineage Gata2, Gata1 genetic expression. Also, ASP facilitated erythroid differentiation via macrophage-mediated EpoR/STAT5 signaling. Conclusions ASP facilitate extramedullary stress erythropoiesis that suggest it might be a promising strategy for stress anemia treatment.

show abstract

“…Sun et al showed that LBP1C-2 extracted from Lycium barbarum was found to alleviate age-related bone loss by targeting BMPRIA/BMPRII/Noggin, which might serve as a new type of botanical medicine to fight age-related osteoporosis [ 45 ]. Several studies have demonstrated that Angelica sinensis could improve rheumatoid arthritis and colitis by reshaping the gut microbiota and also prevent splenic injury and functional impairment by inhibiting oxidative stress and cell apoptosis [ 12 , 46 , 47 ]. It also showed therapeutic potential in ischemic stroke treatment by regulating inflammation and autophagy pathways [ 48 ].…”

Section: Discussionmentioning

confidence: 99%

Association of Drinking Herbal Tea with Activities of Daily Living among Elderly: A Latent Class Analysis

Tao,

Liao,

Zheng

et al. 2023

Nutrients

View full text Add to dashboard Cite

The aim of this study was to explore whether drinking herbal tea and tea would positively benefit activities of daily living (ADL) in the elderly. We used data from the Chinese longitudinal healthy longevity survey (CLHLS) to explore the association. Drinking herbal tea and drinking tea were divided into three groups using latent class analysis (LCA): frequently, occasionally, and rarely. ADL disability was measured by the ADL score. Multivariate COX proportional hazards models with competing risks were used to explore the impact of drinking herbal tea and tea on ADL disability, statistically adjusted for a range of potential confounders. A total of 7441 participants (mean age 81.8 years) were included in this study. The proportions of frequently and occasionally drinking herbal tea were 12.0% and 25.7%, respectively. Additionally, 29.6% and 28.2% of participants reported drinking tea, respectively. Multivariate COX regression showed that compared with rarely drinking, frequently drinking herbal tea could effectively reduce the incidence of ADL disability (HR = 0.85, 95% CI = 0.77–0.93, p = 0.005), whereas tea drinking had a relatively weaker effect (HR = 0.92, 95% CI = 0.83–0.99, p = 0.040). Subgroup analysis found that frequently drinking herbal tea was more protective for males under 80 years old (HR = 0.74 and 0.79, respectively), while frequently drinking tea was somewhat protective for women (HR = 0.92). The results indicate that drinking herbal tea and tea may be associated with a lower incidence of ADL disability. However, the risks associated with using Chinese herb plants still deserve attention.

show abstract

Angelica Sinensis polysaccharide antagonizes 5-Fluorouracil-induced spleen injury and dysfunction by suppressing oxidative stress and apoptosis

Cited by 7 publications

References 52 publications

Ameliorative Effect of Naringenin in 5-Fluorouracil-Induced Hepatotoxicity: An Experimental Study on Albino Wistar Rats

Ameliorative Effect of Naringenin in 5-Fluorouracil-Induced Hepatotoxicity: An Experimental Study on Albino Wistar Rats

Angelica sinensis polysaccharides promote extramedullary stress erythropoiesis via ameliorating splenic glycolysis and EPO/STAT5 signaling-regulated macrophages

Association of Drinking Herbal Tea with Activities of Daily Living among Elderly: A Latent Class Analysis

Contact Info

Product

Resources

About