Angelica sylvestris and Delphinium staphisagria Extracts Induces Antiproliferation through Caspase-mediated Apoptosis on Human Cancer Cells

Akgün, Oğuzhan; Akgun, Halime; Sahin, Cagatay; Çelikler, Serap; Arı, Ferda

doi:10.1590/1678-4324-2022210065

Cited by 4 publications

(1 citation statement)

References 14 publications

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“…Cells labeled with Hoechst 33342, Annexin V and PI were analysed by fluorescence microscopy. In this study, cells were seeded at a density of 5×10 3 cells per well of 96‐well culture plate and treated with Cu(II) flavonoid complex (3.12–25 μM; for 24 hours) and cells were stained described previously [38] . In primary necrotic cells, enlarged nucleus, cell swelling is seen as a result of fluid entry into the cell, while secondary necrotic cells (late apoptosis) are characterized by a pycnotic and fragmented nucleus.…”

Section: Methodsmentioning

confidence: 99%

The Mechanism for Anticancer and Apoptosis‐Inducing Properties of Cu(II) Complex with Quercetin and 1,10‐Phenanthroline

et al. 2022

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This article covers the anticancer activities and mechanisms of action of Cu(II) complexes of flavonoid‐derived quercetin and 1,10‐phenanthroline ligands. The antiproliferative activity of the complex and its ligands was evaluated by MTT, ATP, and SRB viability assays in human lung cancer cells (A549, H1299). Findings for apoptosis were determined by fluorescent staining, flow cytometry analysis, and the M30 antigen method. In addition, the mechanism of action of the complex was investigated by Annexin V staining, caspase 3/7 activity, ROS formation, and cell cycle analysis. The involvement of caspases, thus, apoptosis was confirmed by rescuing cell death by using a pan‐caspase inhibitor (Z‐VAD‐FMK). Again, increased ROS levels in the cell showed that death may occur by apoptosis. For this reason, the accuracy of ROS‐induced apoptosis in cells has been proven as a result of the application of N‐acetylcysteine (NAC), which is a ROS inhibitor. The efficacy of the complex was compared with Cisplatin and ligands. The results showed that the Cu(II) flavonoid complex is cytotoxic on lung cancer cells and may have the potential to act as an effective metal‐based anticancer drug with a lower IC50 over Cisplatin.

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Section: Methodsmentioning

confidence: 99%

The Mechanism for Anticancer and Apoptosis‐Inducing Properties of Cu(II) Complex with Quercetin and 1,10‐Phenanthroline

et al. 2022

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Processing of Angelica (Angelica sylvestris L.) into a functional jam with addition of carob and cinnamon extracts: Evaluation of sensorial, physicochemical, and nutritional characteristics and in vitro bioaccessibility of phenolics

Koç Alibaşoğlu,

Acoğlu Çelik,

Ceylan

et al. 2024

Food Science & Nutrition

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A traditional Angelica jam has been produced from stems of Angelica sylvestris L. growing at the foot of Mount Uludag in Bursa for many years. The aim of this study was to develop new functional formulations of Angelica jam by adding carob and cinnamon extracts, and to evaluate the effect of jam processing and the extracts' addition on the sensory attributes, physicochemical and nutritional characteristics of Angelica jams as well as in vitro bioavailability of phenolics. The main physicochemical properties of the jams including total water‐soluble dry matter (Brix value), pH, invert sugar content, total dietary fiber content, and 5‐(hydroxymethyl) furfural (HMF) ranged between 71.80–72.70 °Bx, 3.65–3.91, 39.30%–49.60%, 0.70–0.90 g/100 g, and 91.00–105.50 mg/kg, respectively. The most abundant minerals were potassium (K), calcium (Ca), magnesium (Mg), phosphorus (P), and iron (Fe) within a concentration range of 80.49–388.67 mg/kg, 111.10–135.00 mg/kg, 24.00–43.28 mg/kg, 7.57–28.06 mg/kg, and 0.52–1.14 mg/kg, respectively. Sensory analysis revealed that general acceptability of Angelica jams in a 9‐point hedonic scale was above 7. The highest antioxidant capacity values of Angelica jam and its functional forms fortified with carob and cinnamon extracts were obtained as 96.68 ± 11.30 mg TE/100 g DM, 248.49 ± 17.78 mg TE/100 g DM, and 193.11 ± 16.06 mg TE/100 g DM, respectively, with the ABTS (2,2′‐azino‐bis‐3‐ethylbenzothiazoline‐6‐sulfonic acid) method. It was observed that the main phenolic compound of the jams was caffeic acid. Depending on the type of extract added into the formulations, trans‐cinnamic acid and gallic acid (GA) became predominant phenolics. Total antioxidant capacity (TAC) of Angelica sylvestris stem decreased by 72%–85% with the heat treatment applied during jam processing. While total phenolic content (TPC) and total antioxidant capacity (measured by the DPPH (1,1‐diphenyl‐2‐picrylhydrazyl) method) increased as a result of intestinal digestion of the jam, it was observed that bioaccessibility of most of the phenolic compounds decreased after intestinal digestion of the jams.

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Angelica sylvestris L. (Apiaceae) of the Isle of Skye (Scotland): chemical composition of essential oil from the aerial flowering parts

Porrello

Merra

Badalamenti

et al. 2023

Natural Product Research

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Angelica sylvestris L. (Apiaceae) of the Isle of Skye (Scotland): chemical composition of essential oil from the aerial flowering partsAngelica is a large genus of plants belonging to Apiaceae family that includes about 100 species of biennial or perennial herbs. Several species of this genus are extensively used in various traditional medicines and, despite their content in toxic furanocoumarins, also as food. In the present study the chemical composition of the essential oil (EO) from aerial flowering parts of Angelica sylvestris L., a plant distributed in Europe, North and Central Asia, collected in the Isle of Skey (Scotland), was analyzed by GC and GC-MS. No one report has been previously published on this accession. The result showed the presence of large quantity of monoterpene hydrocarbons with limonene (51.89%), by far, as the most abundant component. Other metabolites present in lesser quantity were β-pinene (4.61%), α-pinene (3.54%), and thymol (3.33%). Considerations with respect all the other EOs of A. sylvestris taxa, studied so far, were carried out.

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Angelica sylvestris and Delphinium staphisagria Extracts Induces Antiproliferation through Caspase-mediated Apoptosis on Human Cancer Cells

Cited by 4 publications

References 14 publications

The Mechanism for Anticancer and Apoptosis‐Inducing Properties of Cu(II) Complex with Quercetin and 1,10‐Phenanthroline

The Mechanism for Anticancer and Apoptosis‐Inducing Properties of Cu(II) Complex with Quercetin and 1,10‐Phenanthroline

Processing of Angelica (Angelica sylvestris L.) into a functional jam with addition of carob and cinnamon extracts: Evaluation of sensorial, physicochemical, and nutritional characteristics and in vitro bioaccessibility of phenolics

Angelica sylvestris L. (Apiaceae) of the Isle of Skye (Scotland): chemical composition of essential oil from the aerial flowering parts

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