Angelicin regulates LPS-induced inflammation via inhibiting MAPK/NF-κB pathways

Liu, Fang; Sun, Guozhe; Gao, Huayi; Li, Ruisheng; Soromou, Lanan-Wassy; Chen, Na; Deng, Yong; Feng, Hailan

doi:10.1016/j.jss.2013.05.083

Cited by 54 publications

(29 citation statements)

References 31 publications

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“…In response to external stimuli or pathogen macrophages get activated, subsequently other pro‐inflammatory signals get secreted (Laskin and others ). Bacterial LPS is one of the important pro‐inflammatory agents which are responsible for spreading inflammation and leading to several diseases which could cause death (Liu and others ). Here we have used LPS‐stimulated RAW 264.7 cells to study the anti‐inflammation effect of Q32G and QCT isolated from persimmon.…”

Section: Discussionmentioning

confidence: 99%

Anti‐inflammatory Potential of Quercetin‐3‐O‐β‐D‐(“2”‐galloyl)‐glucopyranoside and Quercetin Isolated from Diospyros kaki calyx via Suppression of MAP Signaling Molecules in LPS‐induced RAW 264.7 Macrophages

Cho

Kim

Khan

et al. 2016

Journal of Food Science

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Diospyros kaki (DK) contains an abundance of flavonoids and has been used in folk medicine in Korea for centuries. Here, we report for the first time the anti-inflammatory activities of Quercetin (QCT) and Quercetin 3-O-β-("2"-galloyl)-glucopyranoside (Q32G) isolated from DK. We have determine the no cytotoxicity of Q32G and QCT against RAW 264.7 cells up to concentration of 50 μM. QCT and Q32G demonstrated potent anti-inflammatory activities by reducing expression of nitric oxide (NO), tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6 inducible NO synthase (iNOS), cyclooxygenase (COX)-2, and mitogen-activated protein kinase (MAPKs) in mouse RAW 264.7 macrophages activated with lipopolysaccharide (LPS). Both QCT or Q32G could decrease cellular protein levels of COX-2 and iNOS as well as secreted protein levels of NO, PGE , and cytokines (TNF-α, IL-1β, and IL-6) in culture medium of LPS-stimulated RAW 264.7 macrophages. Immunoblot analysis showed that QCT and Q32G suppressed LPS-induced MAP kinase pathway proteins p-p38, ERK, and JNK. This study revealed that QCT and Q32G have anti-inflammatory potential, however Q32G possess comparable activity as that of QCT and could be use as adjuvant to treat inflammatory diseases.

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Section: Discussionmentioning

confidence: 99%

Anti‐inflammatory Potential of Quercetin‐3‐O‐β‐D‐(“2”‐galloyl)‐glucopyranoside and Quercetin Isolated from Diospyros kaki calyx via Suppression of MAP Signaling Molecules in LPS‐induced RAW 264.7 Macrophages

Cho

Kim

Khan

et al. 2016

Journal of Food Science

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“…We have also found that pyroGlu-Leu decreases plasma TNF-α and IL-6 levels in LPS-administered mice (unpublished data). In D-GalN and LPS-administered animals, NF-κB and MAPKs are activated in macrophages in various tissues (He et al, 2011;Liu et al, 2013;Nakagawa and Maeda, 2012); therefore, pyroGlu-Leu is considered to exert antiinflammatory activity in vivo by the same mechanism as in vitro. Recently, many kinds of functional peptides are demonstrated, but the problem is their instability or short-half life in vivo; many natural peptides are easily hydrolyzed and have limited availability.…”

Section: Discussionmentioning

confidence: 99%

Anti-inflammatory effect of pyroglutamyl-leucine on lipopolysaccharide-stimulated RAW 264.7 macrophages

et al. 2014

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“…Previous studies have suggested that the release of pro-inflammatory cytokines have a central role in VALI (4,5). Nuclear factor (NF)-κB is required for maximal transcription of various cytokines, including tumor necrosis factor (TNF)-α, interleukin (IL)-8, and IL-1β (6,7). Furthermore, it has been suggested that inhibitors of NF-κB function may be useful as anti-inflammatory agents (8).…”

Section: Introductionmentioning

confidence: 99%

Preconditioning of physiological cyclic stretch inhibits the inflammatory response induced by pathologically mechanical stretch in alveolar epithelial cells

Fang

et al. 2017

Exp Ther Med

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Abstract. The aim of the present study was to investigate the effects of preconditioning of physiological cyclic stretch (CS) on the overexpression of early pro-inflammatory cytokines [including tumor necrosis factor (TNF)-α, interleukin (IL)-1β and IL-8] during the inflammatory response induced by pathologically mechanical stretch in lung epithelial cells, and to determine its molecular mechanism of action. Cells were subjected to 5% CS for various durations (0,15, 30, 60 and 120 min) prior to 6 h treatment with pathological 20% CS. In a separate experiment, cells were preconditioned with physiological 5% CS or incubated with a nuclear factor (NF)-κB inhibitor, pyrroldine dithiocarbamate (PDTC). The expression levels of inflammatory mediators were measured using reverse transcription-quantitative polymerase chain reaction. NF-κB was quantified using western blot analysis. Preconditioning with physiological 5% CS for 30, 60 and 120 min was demonstrated to significantly attenuate the release of pathologically mechanical stretch-induced early pro-inflammatory cytokines (TNF-α, IL-1β and IL-8) in alveolar epithelial cells (P<0.05) and significantly reduce the expression of NF-κB (P<0.05). Peak suppression was observed in cells preconditioned for 60 min. In the second set of experiments, it was demonstrated that mechanical stretch-induced release of TNF-α, IL-1β and IL-8 was significantly inhibited by both PDTC pretreatment and 5% CS pretreatment alone (all P<0.05). Furthermore, significant inhibition was also observed when both 5% CS pretreatment and PDTC pretreatment was used on mechanical stretch-induced cells (P<0.05), which was markedly greater than the inhibition induced by either pretreatment alone. The present findings suggest that preconditioning with physiological 5% CS is able to inhibit the inflammatory response induced by pathologically mechanical stretch in alveolar epithelial cells. These anti-inflammatory effects are induced, at least in part, by suppressing the NF-κB signaling pathway.

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Angelicin regulates LPS-induced inflammation via inhibiting MAPK/NF-κB pathways

Cited by 54 publications

References 31 publications

Anti‐inflammatory Potential of Quercetin‐3‐O‐β‐D‐(“2”‐galloyl)‐glucopyranoside and Quercetin Isolated from Diospyros kaki calyx via Suppression of MAP Signaling Molecules in LPS‐induced RAW 264.7 Macrophages

Anti‐inflammatory Potential of Quercetin‐3‐O‐β‐D‐(“2”‐galloyl)‐glucopyranoside and Quercetin Isolated from Diospyros kaki calyx via Suppression of MAP Signaling Molecules in LPS‐induced RAW 264.7 Macrophages

Anti-inflammatory effect of pyroglutamyl-leucine on lipopolysaccharide-stimulated RAW 264.7 macrophages

Preconditioning of physiological cyclic stretch inhibits the inflammatory response induced by pathologically mechanical stretch in alveolar epithelial cells

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