Angiogenesis and its Hormonal Control in the Corpus Luteum of the Pregnant Rat1

Tamura, H.; Greenwald, Gilbert S.

doi:10.1095/biolreprod36.5.1149

Cited by 49 publications

(22 citation statements)

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“…2B), which is consistent with the report by Tamura & Greenwald (1987). Since it is reported that Ang-2 is involved in destabilization of blood vessels and promotes vessel sprouting in the presence of VEGF, angiogenic changes seen in the early luteal phase may be induced by the Ang-2 action because of low Ang-1 expression, although It has been reported that angiogenesis in the CL is activated and parallel to the rapid growth of the CL during mid-pregnancy (Tamura & Greenwald 1987. The present data have also shown an increase in the vascular index in the CL during mid-pregnancy.…”

Section: Discussionsupporting

confidence: 93%

“…Angiogenesis has been recognized to play important roles in the development of the corpus luteum (CL) and maintenance of CL function (Tamura & Greenwald 1987, Smith et al 1994, Ferrara et al 1998, Fraser et al 2000, Sugino et al 2000a, Bowen-Shauver & Gibori 2004, Pauli et al 2005. Previous studies have demonstrated that vascular endothelial growth factor (VEGF) stimulates angiogenesis in the CL and contributes to CL development and progesterone production (Ferrara et al 1998, Fraser et al 2000, Sugino et al 2000a, Pauli et al 2005.…”

Section: Introductionmentioning

confidence: 99%

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Changes in vascular leakage and expression of angiopoietins in the corpus luteum during pregnancy in rats

Matsuoka-Sakata¹,

Tamura²,

Asada³

et al. 2006

Reproduction

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The present study investigates changes in blood vessel stability and its regulation in the corpus luteum (CL) during pregnancy in the rat. First, blood vessel stability in the CL was evaluated during pregnancy based on vascular leakage, which was quantified by the Evans blue assay. Vascular leakage was highest on day 3, thereafter decreased until day 15 and increased again on day 21. Secondly, to study the regulation of vascular leakage, the expression of angiopoietins was examined in the CL during pregnancy. Angiopoietin-1 (Ang-1) effects maturation and stabilization of newly formed blood vessels, while Ang-2 produces the opposite effect by allowing vascular remodeling. An immunohistochemical study showed both Ang-1 and Ang-2 expression in luteal cells. mRNA and protein levels of Ang-1 were significantly higher on days 12 and 15 than those on days 3 and 21, whereas there was no significant change in Ang-2 expression. Since estradiol contributes to CL development during mid-pregnancy, we finally studied whether estradiol regulates vascular leakage and angiopoietin expression. Rats undergoing hypophysectomy and hysterectomy (hypox-hect) on day 12 were treated with estradiol until day 15. Vascular leakage was increased and Ang-1 expression was decreased by hypox-hect, and these effects were completely reversed by estradiol treatment. In conclusion, blood vessel stability in the CL is likely to be associated with CL development and CL regression, and may be regulated by angiopoietins. Estradiol contributes to blood vessel stabilization in the CL during mid-pregnancy, which is associated with an increase in Ang-1 expression.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Changes in vascular leakage and expression of angiopoietins in the corpus luteum during pregnancy in rats

Matsuoka-Sakata¹,

Tamura²,

Asada³

et al. 2006

Reproduction

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“…During the establishment of pregnancy, the corpus luteum rapidly increases its size due to the augmented numbers of endothelial cells and increased luteal blood flow (Bruce et al 1984). The acquirement of corpus luteum function is known to be dependent on the growth of new capillary vessels (Tamura & Greenwald 1987, Smith et al 1994, Ferrara et al 1998, Reynolds et al 2000 and it appears that this prominent vascularization of the corpus luteum may provide the luteal cells with the large amounts of cholesterol that are needed for P4 synthesis as well as aiding the delivery of P4 into the circulation.…”

Section: Introductionmentioning

confidence: 99%

Vascular endothelial growth factor increases messenger RNAs encoding cyclooxygenase-II and membrane-associated prostaglandin E synthase in rat luteal cells

Sakurai¹,

Tamura²,

Kuroda³

2004

Journal of Endocrinology

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Vascular endothelial growth factor (VEGF) is known to be necessary for the vascularization of the developing corpus luteum. Our recent data suggested that cyclooxygenase-II (COX-II) may play a role in the formation of vascular plexuses in developing corpora lutea of the rat. Here we examined the relationship between VEGF and the expression of prostaglandin (PG)-metabolizing enzymes in rat ovarian luteal cells. VEGF treatment caused a dosedependent increase in the expression of COX-II and membrane-associated PGE synthase (mPGES) mRNA in cultured rat luteal cells. However, pretreatment of the luteal cells with a selective COX-II inhibitor, NS-398, abolished the VEGF-enhanced mPGES mRNA expression. VEGF also increased PGE 2 secretion. Conversely, PGE 2 dose-dependently stimulated VEGF mRNA expression. Furthermore, VEGF induced VEGF mRNA expression, but this effect was abolished by NS-398 pretreatment. These findings suggest that VEGF enhances PGE 2 production by stimulating COX-II and mPGES expression in rat corpus luteum and that the effect of VEGF on luteal cells may be partially mediated by this stimulation of PGE 2 production.

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“…A ngiogenesis, the development of vessels from preexisting vessels, is essential for a variety of physiologic processes, including organ development (Adams et al, 1999;Erlebacher et al, 1995), wound healing, and reproduction (Nissen et al, 1998;Tamura and Greenwald, 1987). In adults it is usually related to pathologic conditions such as rheumatoid arthritis, diabetic retinopathy, tumor progression, and metastasis (Ellis and Fidler, 1996;Folkman, 1995).…”

mentioning

confidence: 99%

Effect of Angiogenic and Antiangiogenic Compounds on the Outgrowth of Capillary Structures from Fetal Mouse Bone Explants

Deckers

Pluijm

Dooijewaard

et al. 2001

Laboratory Investigation

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SUMMARY:Fetal mouse metatarsals are well-known models to study cartilage differentiation and osteoclastic resorption. We show here the outgrowth of PECAM-1 positive tubelike structures from the bone rudiments. This feature can be used to study angiogenesis in vitro. The area of outgrowth significantly increased with culture time, as shown by computerized image analysis of PECAM-1 positive tubelike structures. Treatment with recombinant human vascular endothelial growth factor (rhVEGF-A) stimulated the formation of tubelike structures. Treatment of explants with the angiogenesis inhibitor endostatin, the chemokine IP-10, and the thalidomide derivative phatolyl glutamic acid (PG-acid) resulted in an inhibition of the formation of PECAM-1 positive tubelike structures of 48.8% (Ϯ 4%), 50.2% (Ϯ12%), and 80.8% (Ϯ3%), respectively. Outgrowth of tubelike structures was partly dependent on endogenous VEGF-A because treatment with anti-mVEGF-A and truncated VEGF receptor 1 (soluble fms-like tyrosine kinase 1, sFlt1) strongly inhibited the formation of tubelike structures 74% (Ϯ 4%) and 38% (Ϯ 5%), respectively. Neither onset of tube formation nor total area of tubelike structures were changed when metatarsals were cultured on a fibrin gel or collagen type I gel. Tube formation required activation of matrix metalloproteinases because treatment of the bones with an inhibitor of matrix metalloproteinases completely inhibited migration and tube formation, whereas treatment with an inhibitor of plasmin had no effect. In conclusion, we describe a new in vitro model to study angiogenesis that can be used to test the angiogenic or antiangiogenic potential of novel test compounds that also combines the multicellularity of in vivo assays with the accessibility and flexibility of in vitro assays. (Lab Invest 2001, 81:5-15).

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Angiogenesis and its Hormonal Control in the Corpus Luteum of the Pregnant Rat1

Cited by 49 publications

References 0 publications

Changes in vascular leakage and expression of angiopoietins in the corpus luteum during pregnancy in rats

Changes in vascular leakage and expression of angiopoietins in the corpus luteum during pregnancy in rats

Vascular endothelial growth factor increases messenger RNAs encoding cyclooxygenase-II and membrane-associated prostaglandin E synthase in rat luteal cells

Effect of Angiogenic and Antiangiogenic Compounds on the Outgrowth of Capillary Structures from Fetal Mouse Bone Explants

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