Angiogenesis and Lymphangiogenesis in Salivary Gland Adenoid Cystic Carcinoma and Mucoepidermoid Carcinoma

Abstract

Background and Purpose:This study aimed to investigate the immunohistochemical expression of CD31 and podoplanin in order to examine angiogenesis and lymphangiogenesis, respectively in common malignant tumors of salivary glands. Materials and Methods: Forty formalin-fixed, paraffinated blocks (20 adenoid cystic carcinoma and 20 mucoepidermoid carcinoma blocks) were selected from the medical archives of Amir A'lam Hospital of Tehran, Iran. Sections from the blocks were stained by CD31 and D2-40 markers via immunohistochemistry. Clinical and demographic information was extracted from the patients' records. Findings: There was a significant difference between tumors in terms of intratumoral microvessel density (MVD) (P< 0.001), total MVD (P< 0.001), and intratumoral lymphatic vessel density (LVD) (P= 0.011). In mucoepidermoid carcinoma, intratumoral MVD and LVD were greater than peritumoral MVD and LVD (P= 0.001 and P< 0.001, respectively). In mucoepidermoid carcinoma, there was no relationship between histological grade with MVD (total, intratumoral or peritumoral) or LVD (total, intratumoral or peritumoral) (P> 0.05). A similar finding was reported with respect to the histopathological grade of adenoid cystic carcinoma (P> 0.05). Conclusion: The higher level of angiogenesis and lymphangiogenesis in mucoepidermoid carcinoma, specifically at the center of tumor, compared to adenoid cystic carcinoma, may be attributed to differences in the clinical behaviors and metastasis of tumors. Moreover, considering the high LVD at the center of tumor in mucoepidermoid carcinoma and infrequency of metastasis to regional lymph nodes in adenoid cystic carcinoma, it can play a significant role in metastasis to regional lymph nodes.

Podoplanin expression in salivary gland carcinomas and its clinical significance. An immunohistochemical study

“…Also, Americo et al [39] stated that podoplanin was used to stain the cytoplasm and cytoplasmatic membrane of lymphatic endothelial cells in mucoepidermoid carcinoma. Dezfuli et al [40] recorded a cytoplasmic reaction in the tumour cells of mucoepidermoid carcinoma cases in their study.…”

Efeito de quimioterápicos inibidores de histonas deacetilases sobre células-tronco tumorais derivadas de linhagens de carcinoma mucoepidermoide de glândula salivar

“…Além disso, evidências recentes têm apontado para a relação existente entre o aumento das CTTs, sob o estímulo da hipóxia, e o aumento do fator de crescimento endotelial vascular, cujo papel é induzir a angiogênese (198). Interessantemente, apesar desses marcadores serem comumente encontrados em células endoteliais presentes no microambiente tumoral (199), neste trabalho, observou-se que as células derivadas de CME também expressaram esses genes, que por sua vez, tiveram a sua expressão modulada pelos tratamentos com os iHDACs e suas associações com a CIS.…”