Angiogenesis and metastatic potential in breast carcinoma

Ravazoula, Panagiota; Hatjikondi, O.; Kardamakis, D.; Maragoudakis, Michael E.; Bonikos, Dionysis S.

doi:10.1016/s0960-9776(96)90107-1

Cited by 6 publications

(3 citation statements)

References 16 publications

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“…[7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23] These contradictory results are most probably caused by diVerences in methodology. 5 24 Although the counting of microvessels has been made more objective by introducing morphometry 8 45 and image processing, 8 14 19 27-37 the most observer dependent and therefore most subjective step remained-the selection of the hot spot.…”

Section: Discussionmentioning

confidence: 99%

“…2 5 Breast cancer in particular has been studied with regard to the clinical impact of microvessel density (for an overview see Fox 6 ), and the initial studies provided promising results. [7][8][9][10][11][12][13][14][15][16][17] However, these results could not be confirmed in several well conducted studies, and no prognostic value was found. [18][19][20][21][22][23] This can probably be explained mainly by diVerences in methodology.…”

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confidence: 81%

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Fully automated microvessel counting and hot spot selection by image processing of whole tumour sections in invasive breast cancer.

Beliën¹,

Somi²,

Jong³

et al. 1999

J Clin Pathol

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Background-Manual counting of microvessels is subjective and may lead to unacceptable interobserver variability, which may explain conflicting results. Aims-To develop and test an automated method for microvessel counting and objective selection of the hot spot, based on image processing of whole sections, and to compare this with manual selection of a hot spot and counting of microvessels. Methods-Microvessels were stained by CD31 immunohistochemistry in 10 cases of invasive breast cancer. The number of microvessels was counted manually in a subjectively selected hot spot, and also in the same complete tumour sections by interactive and automated image processing methods. An algorithm identified the hot spots from microvessel maps of the whole tumour section. Results-No significant diVerence in manual microvessel counts was found between two observers within the same hot spot, and counts were significantly correlated. However, when the hot spot was reselected, significantly diVerent results were found between repeated counts by the same observer. Counting all microvessels manually within the entire tumour section resulted in significantly diVerent hot spots than manual counts in selected hot spots by the same observer. Within the entire tumour section no significant differences were found between the hot spots of the manual and automated methods using an automated microscope. The hot spot was found using an eight connective path search algorithm, was located at or near the border of the tumour, and (depending on the size of the hot spot) did not always contain the field with the largest number of microvessels. Conclusions-The automated counting of microvessels is preferable to the manual method because of the reduction in measurement time when the complete tumour is scanned, the greater accuracy and objectivity of hot spot selection, and the possibility of visual inspection and relocation of each measurement field afterwards. (J Clin Pathol 1999;52:184-192)

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 81%

Fully automated microvessel counting and hot spot selection by image processing of whole tumour sections in invasive breast cancer.

Beliën¹,

Somi²,

Jong³

et al. 1999

J Clin Pathol

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“…Tumor angiogenesis and MVD were shown to result in the rapid tumor progression and increased invasivity and metastatic activity. The relationship to metastasis was found in non-small-cell lung cancer [ 14 ] and breast carcinoma [ 39 , 40 ]. According to data reported by some investigators [ 41 , 42 ], angiogenesis is an independent prognostic factor in breast carcinoma and prostate cancer.…”

Section: Discussionmentioning

confidence: 99%

Prognostic Value of Microvessel Density in Tumor and Peritumoral Area as Evaluated by CD31 Protein Expression and Argyrophilic Nucleolar Organizer Region Count in Endothelial Cells in Uterine Leiomyosarcoma

Avdalyan

Бобров

Климачев

et al. 2012

Sarcoma

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The objective of this study was to investigate the prognostic value of microvessel density (MVD) in uterine leiomyosarcoma (LMS) and peritumoral area (PA) as evaluated by CD31 expression and argyrophilic nucleolar organizer region (AgNOR) count in endothelial cells. Tissue specimens from 66 patients with uterine LMS were examined. There were no significant differences in the mean MVD between tumor itself and the PA (P = 0.9); moreover, the MVD in the PA often exceeded that in the tumor. No correlation or significant differences were also found in the MVD between different grades of malignancy of LMS (r = 0.1; P = 0.07). The number of AgNORs in tumor endothelial cells was significantly higher in tumor vessels than in the peritumoral area (P < 0.005) and increased with the tumor grade. Analysis of the prognostic value of MVD in uterine LMS and PA showed that the density of tumor vessels was not an independent criterion, while the MVD in the PA affected 10-year survival to a significantly greater extent (χ 2 = 27.5; P = 0.0003). The number of AgNORs also had an important effect on survival of LMS patients: when the threshold of 11.6 granules was exceeded, prognosis was significantly more unfavorable than that prior to exceeding the threshold.

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Relationships between vascularization and proliferation in invasive breast cancer

1999

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Studies on relationships between angiogenesis and tumour cell proliferation have provided conflicting results. This study has therefore investigated the relationships between the number and location of fully automatically identified CD31‐positive microvessels and interactively segmented mitoses and necrotic compartments by image processing. These features were studied in ten invasive breast cancers, in the ‘hot spots’ and in whole tumour sections. Microvessel and mitosis hot spots were topographically close or overlapping and were always located at the periphery of the tumour. The numbers of mitoses and microvessels per mm2 in the hot spot were strongly correlated with the respective numbers in the whole tumour section, as well as mutually. The ratio of mitoses in the hot spot to the whole tumour section was significantly higher than the corresponding microvessel ratio. Mitoses were preferentially located at a distance of 50–150 µm from microvessels. No significant difference was found between the average distance between mitoses and microvessels in the whole tumour sections and in the hot spot (79 vs. 72 µm), although considerable inter‐tumour differences were found (hot spot 43–101 µm, tumour 47–111 µm). The presence of necrotic areas correlated with the number of mitoses per mm2 and necrosis was in general observed at a distance of more than 150 µm from the microvessels, suggesting that necrotic areas have outgrown their vascular system. These results indicate the usefulness of image processing of whole tumour sections for the identification of proliferation and vascularization hot spots, which are strong prognostic factors in breast cancer. The results also support a close relationship between tumour necrosis and microvessels. Copyright © 1999 John Wiley & Sons, Ltd.

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Angiogenesis and metastatic potential in breast carcinoma

Cited by 6 publications

References 16 publications

Fully automated microvessel counting and hot spot selection by image processing of whole tumour sections in invasive breast cancer.

Fully automated microvessel counting and hot spot selection by image processing of whole tumour sections in invasive breast cancer.

Prognostic Value of Microvessel Density in Tumor and Peritumoral Area as Evaluated by CD31 Protein Expression and Argyrophilic Nucleolar Organizer Region Count in Endothelial Cells in Uterine Leiomyosarcoma

Relationships between vascularization and proliferation in invasive breast cancer

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