Angiogenesis Biomarkers May Be Useful in the Management of Patients With Indeterminate Pulmonary Nodules

Serum Biomarkers May Prognosticate Recurrence in Node-Negative, Non-Small Cell Lung Cancers Less Than 4 Centimeters

“…This cross validation is also utilized to compute a variable importance for each biomarker included in the random forests analysis. The random forests methods has been successfully and extensively used for feature selection in biomarker studies, including several reports from our group [13,[16][17][18].…”

“…All serum samples were assayed simultaneously by a blinded technician for serum biomarkers hypothesized to have value for risk stratifying patients for early recurrence. These biomarkers were chosen based on our previous work and sampled biological processes associated with metastatic progression, including angiogenesis, energy metabolism, apoptosis, and inflammation [11][12][13][14][15][16][17][18][19][20][21]. Pretreatment serum specimens were prepared using standard techniques, supplemented with 25 mL/mL mammalian protease inhibitor cocktail (Sigma-Aldrich, St. Louis, MO), and archived at À80 C in aliquots with no specimen subjected to more than two freeze-thaw cycles, as previously described [12,18].…”

“…The biomarkers chosen for investigation in the current study were based on our previous work aimed at the development of blood-based multiple analyte panels to help guide clinical decision making [11][12][13][14][15][16][17][18][19][20][21]. They sample biological processes associated with metastatic progression, including angiogenesis, energy metabolism, apoptosis, and inflammation.…”

Predictors of HbA1c among adipocytokine biomarkers in African American men with varied glucose tolerance

“…All primary data points were collected on a Luminex FLEXMAP 3D® system with concentrations calculated based on 7-point standard curves using a ve-parametric t algorithm in xPONENT® v4.0.3 (Luminex Corp., Austin, TX). All data met minimum quality control thresholds de ned by the kit manufacturer with percent coe cient of variation (%CV) values ≤10, all as previously de ned [45,46].…”

“…Adipocytokine biomarkers were measured at the Rush Biomarker development Core research laboratory, applying laboratory standards of care and references [39,45,46]. A total 14 serum adipocytokine biomarkers were explored in the Luminex immunobead platform using commercially available kits that were implemented according to the manufacturer's recommended protocols.…”