Angiogenesis factors in ocular tissues of normal rabbits on chorioallantoic membrane assay.

Okamoto, Takuya; Oikawa, Shinichi; Toyota, Takayoshi; Gotō, Yoshio

doi:10.1620/tjem.154.63

Cited by 8 publications

(4 citation statements)

References 18 publications

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“…They may be due to the differences in experimental designs between 311-thymidine incorporation study (incubation for 18 hr) and cell number measurement study (cluture for 5 days). Thus, the retinal extracts of rabbit possessed mitogenic activity as well as angiogenic activity previously reported by us using chorioallantoic membrane (Okamoto et al 1988).…”

Section: Discussionsupporting

confidence: 73%

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Effect of eye-derived growth factors from rabbit retina on proliferation of endothelial cell from marginal vessels.

Okamoto

Oikawa²,

Toyota³

1989

Tohoku J. Exp. Med.

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Section: Discussionsupporting

confidence: 73%

“…We have already reported on angiogenic activity in the retinal extracts of normal rabbits (Okamoto et al 1988). Rabbits are easily made experimental diabetes by an injection of diabtogenic substances.…”

mentioning

confidence: 94%

Effect of eye-derived growth factors from rabbit retina on proliferation of endothelial cell from marginal vessels.

Okamoto

Oikawa²,

Toyota³

1989

Tohoku J. Exp. Med.

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“…ancer biologists, developmental biologists, and ophthalmologists have described the chick chorioallantoic membrane (CAM) as a model system for studying development, 1 cancer behavior, [2][3] properties of biomaterials, 4 angiogenesis, [5][6][7][8][9][10] and photodynamic therapy. 11 We propose several new applications for CAM in the study of retina and its vasculature with respect to microsurgical interventions.…”

mentioning

confidence: 99%

The Chick Chorioallantoic Membrane as a Model Tissue for Surgical Retinal Research and Simulation

Leng

Miller

Bilbao

et al. 2004

Retina

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Purpose:We describe the use of chick chorioallantoic membrane (CAM) as a model system for the study of the precision and safety of vitreoretinal microsurgical instruments and techniques.Methods: The CAM was prepared for experimentation with and without its inner shell membrane (ISM) attached for in vivo and in vitro experiments that simulated medical and surgical interventions on the retina.Results: The CAM's ease of use, low cost, and anatomic structure make it a convenient model for surgical retinal and retinal vascular modeling.Conclusion: While CAM has been used extensively in the past for ocular angiogenesis studies, we describe the tissue as a useful tool for a variety of other applications, including (1) testing of novel surgical tools and techniques for cutting and coagulating retina and its vasculature, (2) testing vessel cannulation and injection techniques, (3) angiographic studies, and (4) endoscopic surgery.

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“…They observed a potent vasoproliferative response when pellets containing retinal extracts were applied on the top of the CAM, while other adult tissues resulted ineffective ( 99 ). With a similar approach, Okamoto and colleagues demonstrated that extracts derived from rabbit retina, iris-ciliary body, and optic nerve exerted an angiogenic activity on CAM, with retinal extracts inducing the strongest effect ( 100 ). On these bases, the CAM assay was applied for testing angiogenic factors extracted from both cat and bovine retinas ( 101 ), and Prost compared the angiogenic activity of the detached retina with that of the normal attached retina, demonstrating that the detached retina exhibits a stronger angiogenic activity ( 102 ).…”

Section: The Chick Embryo Chorioallantoic Membrane For Diabetic Retinmentioning

confidence: 99%

Angiogenesis-Inflammation Cross Talk in Diabetic Retinopathy: Novel Insights From the Chick Embryo Chorioallantoic Membrane/Human Vitreous Platform

et al. 2020

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Pathological angiogenesis of the retina is a key component of irreversible causes of blindness, as observed in proliferative diabetic retinopathy (PDR). The pathogenesis of PDR is complex and involves vascular, inflammatory, and neuronal mechanisms. Several structural and molecular alterations associated to PDR are related to the presence of inflammation that appears to play a non-redundant role in the neovascular response that characterizes the retina of PDR patients. Vascular endothelial growth factor (VEGF) blockers have evolved over time for the treatment of retinal neovascularization. However, several limitations to anti-VEGF interventions exist. Indeed, the production of other angiogenic factors and pro-inflammatory mediators may nullify and/or cause resistance to anti-VEGF therapies. Thus, appropriate experimental models are crucial for dissecting the mechanisms leading to retinal neovascularization and for the discovery of more efficacious anti-angiogenic/anti-inflammatory therapies for PDR patients. This review focuses on the tight cross talk between angiogenesis and inflammation during PDR and describe how the chick embryo chorioallantoic membrane (CAM) assay may represent a cost-effective and rapid in vivo tool for the study of the relationship between neovascular and inflammatory responses elicited by the vitreous humor of PDR patients and for the screening of novel therapeutic agents.

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Angiogenesis factors in ocular tissues of normal rabbits on chorioallantoic membrane assay.

Cited by 8 publications

References 18 publications

Effect of eye-derived growth factors from rabbit retina on proliferation of endothelial cell from marginal vessels.

Effect of eye-derived growth factors from rabbit retina on proliferation of endothelial cell from marginal vessels.

The Chick Chorioallantoic Membrane as a Model Tissue for Surgical Retinal Research and Simulation

Angiogenesis-Inflammation Cross Talk in Diabetic Retinopathy: Novel Insights From the Chick Embryo Chorioallantoic Membrane/Human Vitreous Platform

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