Angiogenesis in Rat Uterine Cicatrix after Injection of Autologous Bone Marrow Mesenchymal Stem Cells

Abstract

Results of injection of autologous bone marrow mesenchymal stem cells with transfected GFP gene into the rat uterine horn cicatrix were studied by light microscopy. Large groups of blood vessels with blood cells inside were seen after injection of autologous bone marrow cells into the cicatrix on the right horn, formed 2 months after its ligation; no groups of vessels of this kind were found in the cicatrix in the contralateral horn. Examination of unstained sections in reflected UV light showed sufficiently bright fluorescence in the endothelium and outer vascular membrane in the uterine horn cicatrix only on the side of injection. Hence, autologous mesenchymal stem cells injected into the cicatrix formed the blood vessels due to differentiation into endotheliocytes and pericytes. The expression of GFP gene not only in the vascular endothelium, but also in vascular outer membranes indicated that autologous mesenchymal stem cells differentiated in the endothelial and pericytic directions.

Effect of Bone Marrow Derived Mesenchymal Stem Cells on Healing of Induced Full-Thickness Skin Wounds in Albino Rat

“…These findings might be attributed to the role of BMSCs in neovascularisation to sustain the newly formed granulation tissue (8,24,26). BMSCs could differentiate into endothelial cells and pericytes (27). In the present work, this assumption might be supported by detection of PKH26 labeled MSCs within & around the regenerated blood vessels in the regenerated dermis of MSCs-treated groups and negative detection of fluorescence in any section of non treated group.…”

Therapeutic effect of human umbilical cord-derived mesenchymal stem cells on injured rat endometrium during its chronic phase

“…MSCs migrate to the injured site to produce a large number of proinflammatory factors, anti-inflammatory cytokines, and growth factors [ 23 – 25 ]. Here, we detected the expression of the proinflammatory factors interferon (IFN)-γ, tumor necrosis factor (TNF)-α, and interleukin (IL)-2, and the anti-inflammatory cytokines IL4 and IL-10 by qRT-PCR (Fig.…”

The Effect of Adipose Tissue-Derived Mesenchymal Stem Cells on Regeneration of Uterine Scars

“…The effects of autologous stem cells of bone marrow origin on the scar tissue of rat uterus were examined with the light microscope and observed that it increased angiogenesis (25).…”

“…Taking the histologic structure and embryonic origin of the uterus into consideration, similarities between surface markers of the MSCs with uterus have been determined in a limited number of studies conducted in this area. These cells have a differentiation ability in similar quality to normal tissue in the uterus and new tissue formation including less fibrotic tissue have been observed (24,25). Of the MSCs that will be used for this purpose, MSCs of adipose tissue origin are applicable and easy to obtain, are prepared with a different method than the methods tried in many studies so far, and it is used in our study in the experimental uterine scar model in the rat uterus.…”