Angiogenesis inhibition by a lichen compound olivetoric acid

Koparal, Ayşe Tansu; Ulus, Gönül; Zeytinoğlu, Melih; Tay, Turgay; Türk, Ayşen Özdemir

doi:10.1002/ptr.3035

Cited by 35 publications

(39 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Certain lichen secondary compounds (ca. 1000 presently known) derived from fungal metabolism have antimicrobial, antiviral, antiprotozoal, antiproliferative, antitumor, antiinflammatory, anti-pyretic, analgesic, photoprotective, enzyme inhibitory and anti-angiogenic properties [4][5][6][7][8]. Usnic acid, a dibenzofuran derivative, is one of the most frequent and most studied lichen metabolites [9].…”

Section: Introductionmentioning

confidence: 99%

Anti-angiogenic and antiproliferative properties of the lichen substances (-)-usnic acid and vulpinic acid

Koparal

2015

Zeitschrift Für Naturforschung C

View full text Add to dashboard Cite

The anti-proliferative activities of the lichen substances (-)-usnic acid and vulpinic acid on the viability of HepG2 hepatocarcinoma cells, NS20Y neuroblastoma cells and HUVEC endothelial cells were studied by the MTT assay. The anti-angiogenic potential of the substances was determined by the endothelial tube formation assay. Both lichen substances exhibited strong anti-angiogenic activity and were more cytotoxic to the cancer cell lines than to the normal cell line, but vulpinic acid has more potential as an anti-angiogenic substance because of its low cytotoxicity and stronger anti-angiogenic activity on the HUVEC cell line.

show abstract

Section: Introductionmentioning

confidence: 99%

Anti-angiogenic and antiproliferative properties of the lichen substances (-)-usnic acid and vulpinic acid

Koparal

2015

Zeitschrift Für Naturforschung C

View full text Add to dashboard Cite

show abstract

“…Currently, 1050 secondary metabolites are known which are unique to lichens (Stocker-Worgotter, 2008; Verma & Behera, 2015). Bioactivity studies on lichen metabolites reported in recent years indicate their therapeutic potential in terms of natural antioxidants, antimicrobial, anticancer, probiotic, anti-angiogenic, and cardiovascular protective agents Gaikwad et al, 2014;Koparal et al, 2010;Mahadik et al, 2011;Srivastava et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Radical scavenging, prolyl endopeptidase inhibitory, and antimicrobial potential of a cultured Himalayan lichenCetrelia olivetorum

Savale

Pol

Khare

et al. 2015

Pharmaceutical Biology

View full text Add to dashboard Cite

Context: Lichens are source of natural bioactive compounds which are traditionally used to cure a variety of ailments. Objective: The objective of this study is to assess free radical scavenging, prolyl endopeptidase inhibitory (PEPI), and antimicrobial potential of a high altitude lichen species Cetrelia olivetorum (Nyl.) W. L. Culb. & C. F. Culb (Parmeliaceae). Materials and methods: Lichen C. olivetorum has been cultured in vitro, and optimized culture conditions were implemented in bioreactor to obtain high quantity of biomass for the study of radical scavenging, PEPI, and antimicrobial activities. Radical scavenging activity of methanol extract of Cetrelia olivetorum (MECO) was tested at 100 mg/mL, PEPI activity at 25 and 50 mg/mL, and antimicrobial activity at 5, 25, 50, and 100 mg/mL conc. All the biological activities of natural thallus extract and its derived culture extract were evaluated spectrophotometrically. Results: Murashige and Skoog medium supplemented with 3% glucose and 100 ppb indole-3-butyric acid (IBA) supported biomass growth at flask level and yielded 5.095 g biomass in bioreactor. MECO of both the cultured and the natural lichen exhibited half inhibiting concentration (IC 50 ) for radical scavenging activities in the range of 50-60 mg/mL, whereas the IC 50 value of standard antioxidants was found to be in the range of 12-29 mg/mL. The IC 50 value of lichen extract for PEPI activity was 144-288 mg/mL, whereas the IC 50 value of standard prolyl endopeptidase inhibitor, Z-pro-prolinal, was 57.73 mg/mL. As far as the antimicrobial activity of MECO is concerned, minimum inhibitory concentration (MIC) value of lichen extracts against tested microorganisms was obtained in the range of 50-104 mg/mL and found to be more effective than commercially available standard erythromycin. Discussion: Murashige and Skoog medium containing IBA was found to be suitable for maximum biomass production of C. olivetorum under bioreactor conditions. The cultured lichen biomass extract also showed antioxidant, PEPI, and antimicrobial potential. Conclusion: The present study indicates therapeutic potential of Himalayan lichen C. olivetorum against neurodegenerative diseases owing to its radical scavenging, PEPI, and antimicrobial activities. Further, the result encourages its commercial exploitation through mass culture for production of its bioactive components and their use in pharmaceutical and nutraceutical industries.

show abstract

“…The latest ones in these activities have been anticancer, antimicrobial and antioxidant. Koparal et al (2010) investigated antiangiogenic activity of OLA isolated from P. furfuracea and reported that reduction in endothelial cells occurred as OLA broke structure of the actin cytoskeleton. Inhibitory activities of bacteria growth of OLA were reported by some researchers (Türk et al 2006;Mitrović et al 2014).…”

Section: Discussionmentioning

confidence: 99%

In vitroantitumor activities of the lichen compounds olivetoric, physodic and psoromic acid in rat neuron and glioblastoma cells

Emsen

Aslan

Toğar

et al. 2015

Pharmaceutical Biology

View full text Add to dashboard Cite

Context Since methods utilised in the treatment of glioblastoma multiforme (GBM) are inadequate and have too many side effects, usage of herbal products in the treatment process comes into prominence. Lichens are symbiotic organisms used for medicinal purposes for many years. There are various anticancer treatments about components of two lichen species used in the present study. Objective Antitumor potential of three lichen secondary metabolites including olivetoric acid (OLA) and physodic acid (PHA) isolated from Pseudevernia furfuracea (L.) Zopf (Parmeliaceae) and psoromic acid (PSA) isolated from Rhizoplaca melanophthalma (DC.) Leuckert (Lecanoraceae) were investigated on human U87MG-GBM cell lines and primary rat cerebral cortex (PRCC) cells for the first time. Materials and methods PRCC cells used as healthy brain cells were obtained from SpragueDawley rats. The treatments were carried out on the cells cultured for 48 h. Cytotoxic effects of different concentrations (2.5, 5, 10, 20 and 40 mg/L) of metabolites on the cells were determined via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase (LDH) analyses. Total antioxidant capacity (TAC) and total oxidant status (TOS) parameters were used for assessing oxidative alterations. Oxidative DNA damage potentials of metabolites were investigated via evaluating 8-hydroxy-2 0 -deoxyguanosine (8-OH-dG) levels. Results Median inhibitory concentration (IC 50 ) values of OLA, PHA and PSA were 125.71, 698.19 and 79.40 mg/L for PRCC cells and 17.55, 410.72 and 56.22 mg/L for U87MG cells, respectively. It was revealed that cytotoxic effects of these metabolites showed positive correlation with concentration, LDH activity and oxidative DNA damage. Discussion and conclusion The present findings obtained in this study revealed that primarily OLA and then PSA had high potential for use in the treatment of GBM. ARTICLE HISTORY

show abstract

Angiogenesis inhibition by a lichen compound olivetoric acid

Cited by 35 publications

References 30 publications

Anti-angiogenic and antiproliferative properties of the lichen substances (-)-usnic acid and vulpinic acid

Anti-angiogenic and antiproliferative properties of the lichen substances (-)-usnic acid and vulpinic acid

Radical scavenging, prolyl endopeptidase inhibitory, and antimicrobial potential of a cultured Himalayan lichenCetrelia olivetorum

In vitroantitumor activities of the lichen compounds olivetoric, physodic and psoromic acid in rat neuron and glioblastoma cells

Contact Info

Product

Resources

About