Angiogenic activity from bovine retina: partial purification and characterization.

Abstract

Bovine retinas contain a factor that stimulates proliferation of aortic endothelial cells in culture as well as neovascularization on the chicken chorioallantoic membrane. The stimulatory activity has been partially purified from a balanced salt solution extract of bovine retinas. The stability of the activity to acid pH was utilized as the first step in purification. The acidtreated material was subjected to ion-exchange chromatography on DEAE Bio-Gel A. The material thatwas eluted with 0.

Isolation, characterization, and localization of heparin-binding growth factors in the heart.

“…In culture, bFGF is found in endothelial cells (9)(10)(11) and many other normal and transformed cell types, with some exceptions (12)(13)(14)(15). aFGF is essentially restricted to brain (5,16) and retina (17), with small amounts identified in kidney (18) and bone matrix (19). In culture it is expressed by smooth muscle (20) and rhabdomyosarcoma (21) cells, but not by several other cell types (12)(13)(14).…”

“…All samples were adjusted to equal ionic strengths, usually 0.8 M, reduced with beta mercaptoethanol, and heated to 90°C for 3 min. Additional molecular weight markers were human recombinant (hr) bFGF and aFGF (154 amino acid constructs), which usually migrate at 17 45 min in a Polyblot semi-dry apparatus (American Bionetics, Hayward, CA) using the manufacturer's specified anode and cathode buffers. The proteins were then stained as described below.…”

Purification of a factor from human placenta that stimulates capillary endothelial cell protease production, DNA synthesis, and migration.

“…It has been proposed that the response of microvascular endothelial cells to angiogenic factors has three major components: an increase in endothelial cell protease production that allows the endothelial cells to penetrate the surrounding tissues, a stimulation of endothelial cell migration toward the source of the angiogenic factor, and an increase in the rate of endothelial cell proliferation (2). Possible correlates of each of these components have been identified in the response of cultured microvascular endothelial cells to angiogenic preparations-increased collagenase and plasminogen activator (PA) production (2), increased endothelial cell motility and chemotaxis (3,4), and increased rate of multiplication (5,6). However, it has not been demonstrated whether the stimulation of these different processes is due to a single molecule in the preparations or to several different molecules.…”

Purification from a human hepatoma cell line of a basic fibroblast growth factor-like molecule that stimulates capillary endothelial cell plasminogen activator production, DNA synthesis, and migration.

“…Angiogenic preparations from a number of cells and tissues have been reported to induce a phenotype in cultured capillary endothelial cells which consists of increased mitosis (5,6,21,22), chemotactic movement (2,16,24), and production of proteases (12). Each of these responses has been used in assays for the detection of putative angiogenesis factors.…”