Angiogenic activity of cytochalasin B-induced membrane vesicles of human mesenchymal stem cells

Abstract

Background: The cytochalasin B-induced membrane vesicles (CIMVs) are suggested to be used as a vehicle for the delivery of therapeutics. However, the angiogenic activity and therapeutic potential of human mesenchymal stem cells (MSCs) derived CIMVs (CIMVs-MSCs) remains unknown. Objectives: The objectives of this study were to analyzed the morphology, size distribution, molecular composition and angiogenic properties of CIMVs-MSCs. Methods: The morphology of CIMVs-MSC was analyzed by scanning electron microscopy. The proteomic analysis, multiplex analysis and immunostaining were used to characterize the molecular composition of the CIMVs-MSCs. The transfer of surface proteins from a donor to a recipient cell mediated by CIMVs-MSCs was demonstrated using immunostaining and confocal microscopy. The angiogenic potential of CIMVs-MSCs was evaluated using in vivo approach of subcutaneous implantation of CIMVs-MSCs in mixture with Matrigel matrix.

Cytochalasin B‐Induced Membrane Vesicles of NK Cells for Efficient Tumor Immunotherapy

“…As vesicles contain intracellular proteins and are formed outwardly, the proteome of vesicles could be distinct between naïve EV and artificially induced CIMV. [24,25] The majority of NK cell-derived vesicles possessed an immunological phenotype equivalent to that of parental NK cells, as shown in previous studies, [26][27][28] or even improved protein contents with granzyme B and FasL in NK-CIMVs.…”

Photo-induced synthesis and antitumor activity of marine zygosporamide analogs containing the isoindolinone fragment

“…9 The isoindolinone fragment can be introduced into the backbone via the photoinduced SET reaction to synthesize cyclic peptides, and is the active structure of many antitumor small molecules and increases the rigidity of the cyclic peptide for improving the stability. 10,11 In this paper, we engineered zygosporamide cyclic peptides via the photo-induced SET reaction in which the D-leucine and adjacent L-leucine caught our attention. Consequently, we designed and synthesized two novel marine cyclic peptide zygosporamide analogs: the first one was to be synthesized without changing the original amino acid configuration; the other was to exchange R-3 Leu and S-4 Leu to determine its activity (Fig.…”