Angiogenic activity of endothelial progenitor cells through angiopoietin-1 and angiopoietin-2

Siavashi, Vahid; Sariri, Reyhaneh; Nassiri, Seyed Mahdi; Esmaeilivand, Masoumeh; Asadian, Simin; Cheraghi, Hadi; Barekati-Mowahed, Mazyar; Rahbarghazi‬, Reza

doi:10.1080/19768354.2016.1189961

Cited by 22 publications

(25 citation statements)

References 40 publications

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“…Based on previous observations, the critical role of these receptors has been documented in the context of vascularization and angiogenesisassociated signaling pathways (Jeltsch, Leppänen, Saharinen, & Alitalo, 2013;Vahid Siavashi, Nassiri, Esmaeiliv, & Rahbarghazi, 2016). Tie and VEGF receptors are largely confined to endothelial lineage and their activation elicits ECs survival and remodeling of pre-existing vascular bed (Jeltsch et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Alginate‐gelatin encapsulation of human endothelial cells promoted angiogenesis in in vivo and in vitro milieu

Nemati

Rezabakhsh

Khoshfetrat

et al. 2017

Biotech & Bioengineering

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Up to present, many advantages have been achieved in the field of cell-based therapies by applying sophisticated methodologies and delivery approaches. Microcapsules are capable to provide safe microenvironment for cells during transplantation in a simulated physiological 3D milieu. Here, we aimed to investigate the effect of alginate-gelatin encapsulation on angiogenic behavior of human endothelial cells over a period of 5 days. Human umbilical vein endothelial cells were encapsulated by alginate-gelatin substrate and incubated for 5 days. MTT and autophagy PCR array analysis were used to monitor cell survival rate. For in vitro angiogenesis analysis, cell distribution of Tie-1, Tie-2, VEGFR-1, and VEGFR-2 were detected by ELISA. In addition to in vitro tubulogenesis assay, we monitored the expression of VE-cadherin by Western blotting. The migration capacity of encapsulated HUVECs was studied by measuring MMP-2 and MMP-9 via gelatin zymography. The in vivo angiogenic potential of encapsulated HUVECs was analyzed in immune-compromised mouse implant model during 7 days post-transplantation. We demonstrated that encapsulation promoted HUVECs cell survival and proliferation. Compared to control, no significant differences were observed in autophagic status of encapsulated cells (p > 0.05). The level of Tie-1, Tie-2, VEGFR-1, and VEGFR-2 were increased, but did not reach to significant levels. Encapsulation decreased MMP-2, -9 activity and increased the VE-cadherin level in enclosed cells (p < 0.05). Moreover, an enhanced in vivo angiogenic response of encapsulated HUVECs was evident as compared to non-capsulated cells (p < 0.05). These observations suggest that alginate-gelatin encapsulation can induce angiogenic response in in vivo and in vitro conditions.

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Section: Discussionmentioning

confidence: 99%

Alginate‐gelatin encapsulation of human endothelial cells promoted angiogenesis in in vivo and in vitro milieu

Nemati

Rezabakhsh

Khoshfetrat

et al. 2017

Biotech & Bioengineering

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“…The methodological procedure is summarized in Figure . To further verify these cells, binding of Ulex europaeus agglutinin (UEA‐1) and uptake of 1,1′‐dioctadecyl‐3,3,3',3'‐tetramethylindocarbocyanine (Dil)‐labeled acetylated LDL (Dil‐Ac‐LDL) was assessed as described previously [Siavashi et al, ,]. For this purpose, cells were washed with PBS and incubated in the M199 medium containing 10 μg/ml of Dil‐Ac‐LDL (Cat No: L‐3485, Gibco) for 4 h and then stained with FITC‐labeled UEA‐1 (Cat No: L2895; Sigma‐Aldrich, MO) for 4 h. 4′,6‐diamidino‐2‐phenylindole (DAPI, 1 mg/ml, Cat No:D9542; Sigma‐Aldrich) was used for nuclear counterstaining.…”

Section: Methodsmentioning

confidence: 99%

Endothelial Progenitor Cell Mobilization in Preterm Infants With Sepsis Is Associated With Improved Survival

Siavashi

Asadian

Taheri-Asl³

et al. 2017

J of Cellular Biochemistry

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Microvascular dysfunction plays a key role in the pathology of sepsis, leading to multi-organ failure, and death. Circulating endothelial progenitor cells (cEPCs) are critically involved in the maintenance of the vascular homeostasis in both physiological and pathological contexts. In this study, concentration of cEPCs in preterm infants with sepsis was determined to recognize whether the EPC mobilization would affect the clinical outcome of infantile sepsis. One hundred and thirty-three preterm infants (81 with sepsis and 52 without sepsis) were enrolled in this study. The release of EPCs in circulation was first quantified. Thereafter, these cells were cultivated and biological features of these cells such as, proliferation and colony forming efficiency were analyzed. The levels of chemoattractant cytokines were also measured in infants. In mouse models of sepsis, effects of VEGF and SDF-1 as well as anti-VEGF and anti-SDF-1 were evaluated in order to shed light upon the role which the EPC mobilization plays in the overall survival of septic animals. Circulating EPCs were significantly higher in preterm infants with sepsis than in the non-sepsis group. Serum levels of VEGF, SDF-1, and Angiopoietin-2 were also higher in preterm infants with sepsis than in control non-sepsis. In the animal experiments, injection of VEGF and SDF-1 prompted the mobilization of EPCs, leading to an improvement in survival whereas injection of anti-VEGF and anti-SDF-1 was associated with significant deterioration of survival. Overall, our results demonstrated the beneficial effects of EPC release in preterm infants with sepsis, with increased mobilization of these cells was associated with improved survival. J. Cell. Biochem. 118: 3299-3307, 2017. © 2017 Wiley Periodicals, Inc.

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“…Flow cytometry assays were performed as previously described with some modifications [Siavashi et al, ]. For this purpose, MNCs or cEPCs expanded on fibronectin were incubated with a panel of antibodies, including PE‐conjugated anti‐human CD309 (VEGFR2 Flk‐1) (Cat No: 359903; Biolegend), FITC‐conjugated anti‐human CD‐34 (Cat No: ab46924, abcam, Inc.), and APC‐conjugated anti‐human CD133 antibodies (Cat No: 130‐098‐829; Miltenyi Biotech, Auburn, CA).…”

Section: Methodsmentioning

confidence: 99%

Circulation Enrichment of Functional Endothelial Progenitor Cells by Infantile Phototherapy

Siavashi¹,

Asadian

Sharifi

et al. 2016

J. Cell. Biochem.

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Phototherapy is the most common therapy used for severe jaundice. There is increasing evidence that phototherapy can directly affect the expression and function of cell surface receptors including adhesion molecules, cytokines, and growth factor receptors. In this study, the effect of two infantile phototherapy regimens, including single and intensive phototherapy was investigated on biological features of circulation endothelial progenitor cells (cEPCs), as well as on serum secretion of two important chemotactic cytokines, SDF-1 and VEGF. Sixty infants diagnosed with severe hyperbilirubinemia and exposed to phototherapy were enrolled in this study. cEPCs were isolated before and after phototherapy and then migratory, proliferative, tubulogenic, and functional properties of these cells were analyzed. Our results revealed that intensive phototherapy markedly increased the release of EPCs into the circulation, and augmented the serum concentrations of both SDF-1 and VEGF cytokines. Cell proliferation, tubulogenic, and migratory properties of cEPCs isolated and expanded from infants with intensive phototherapy were significantly improved. cEPCs from infants with intensive phototherapy also showed greater levels of acetylated low-density lipoprotein and lectin binding. Overall, our results showed that the intensive phototherapy regimen can mobilize functional EPCs into the circulation through up-regulation of serum levels of VEGF and SDF-1, indicating phototherapy as an effective modality for improvement of stem cell mobilization in the therapeutic regenerative medicine. J. Cell. Biochem. 118: 330-340, 2017. © 2016 Wiley Periodicals, Inc.

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Angiogenic activity of endothelial progenitor cells through angiopoietin-1 and angiopoietin-2

Cited by 22 publications

References 40 publications

Alginate‐gelatin encapsulation of human endothelial cells promoted angiogenesis in in vivo and in vitro milieu

Alginate‐gelatin encapsulation of human endothelial cells promoted angiogenesis in in vivo and in vitro milieu

Endothelial Progenitor Cell Mobilization in Preterm Infants With Sepsis Is Associated With Improved Survival

Circulation Enrichment of Functional Endothelial Progenitor Cells by Infantile Phototherapy

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