Angiogenic Activity of Sera from Extrinsic Allergic Alveolitis Patients in Relation to Clinical, Radiological, and Functional Pulmonary Changes

Zielonka, Tadeusz M.; Demkow, Urszula; Filewska, M; Białas, B; Życińska, Katarzyna; Radzikowska, E; Wardyn, Andrzej K; Skopińska-Różewska, Ewa

doi:10.1007/s00408-010-9228-5

Cited by 7 publications

(12 citation statements)

References 27 publications

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“…To assess the effect of sera from TB patients on angiogenesis induced by different fractions of MNC, a leukocyte-induced angiogenesis test described by Sidky and Auerbach in an animal model [27] with modification [10, 28] was used. The local ethical commission for experimenting on animals approved all procedures involved in this study.…”

Section: Methodsmentioning

confidence: 99%

Angiogenic Activity of Sera from Pulmonary Tuberculosis Patients in Relation to IL-12p40 and TNFα Serum Levels

Zielonka

Demkow

Michałowska-Mitczuk³

et al. 2011

Lung

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The role of angiogenesis in the pathogenesis of tuberculosis (TB) is not clear. The aim of this study was to examine the effect of sera from TB patients on angiogenesis induced by different subsets of normal human mononuclear cells (MNC) in relation to IL-12p40 and TNFα serum levels. Serum samples from 36 pulmonary TB patients and from 22 healthy volunteers were evaluated. To assess angiogenic reaction the leukocytes-induced angiogenesis test according to Sidky and Auerbach was performed. IL-12p40 and TNFα serum levels were evaluated by ELISA. Sera from TB patients significantly stimulated angiogenic activity of MNC compared to sera from healthy donors and PBS (p < 0.001). The number of microvessels formed after injection of lymphocytes preincubated with sera from TB patients was significantly lower compared to the number of microvessels created after injection of MNC preincubated with the same sera (p < 0.016). However, the number of microvessels created after the injection of lymphocytes preincubated with sera from healthy donors or with PBS alone was significantly higher (p < 0.017). The mean levels of IL-12p40 and TNFα were significantly elevated in sera from TB patients compared to healthy donors. We observed a correlation between angiogenic activity of sera from TB patients and IL-12p40 and TNFα serum levels (p < 0.01). Sera from TB patients constitute a source of mediators that participate in angiogenesis and prime monocytes for production of proangiogenic factors. The main proangiogenic effect of TB patients’ sera is mediated by macrophages/monocytes. TNFα and IL-12p40 may indirectly stimulate angiogenesis in TB.

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Section: Methodsmentioning

confidence: 99%

Angiogenic Activity of Sera from Pulmonary Tuberculosis Patients in Relation to IL-12p40 and TNFα Serum Levels

Zielonka

Demkow

Michałowska-Mitczuk³

et al. 2011

Lung

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“…Autoimmunity has been reported as a source of factors modulating angiogenesis, and proangiogenic effects of sera from patients with systemic diseases depend on the intensity of radiological signs of a pulmonary pathology [19]. On the other hand, the intensity of proangiogenic stimulation does not correlate with dyspnea or lung function tests [19].…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…On the other hand, the intensity of proangiogenic stimulation does not correlate with dyspnea or lung function tests [19]. However, a suggestion for an association of autoimmunity, pulmonary pathology, and modulation of angiogenesis has been made [19]. Proangiogenic factors, such as vascular endothelial growth factor (VEGF), enhance vascular permeability and increase blood-brain barrier leakage and these factors' expression is increased in autoimmune central nervous system diseases [20].…”

Section: Discussionmentioning

confidence: 99%

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Onconeuronal and antineuronal antibodies in patients with neoplastic and non-neoplastic pulmonary pathologies and suspected for paraneoplastic neurological syndrome

Michalak

Cofta²,

Piatek³

et al. 2009

Eur J Med Res

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ObjectiveOnconeuronal antibodies are important diagnostic tool in patients with suspicion of paraneoplastic neurological syndromes (PNS). However, their role in PNS pathophysiology and specificity for particular neurological manifestation remains unclear. The aim of this study was to evaluate onconeuronal and antineuronal antibodies in patients with pulmonary pathologies and suspected for PNS.Materials and methodsTwenty one patients with pulmonary pathologies were selected from the database of 525 consecutive patients with suspicion of PNS. Patients' sera were screened for the presence of onconeuronal and antineuronal antibodies by means of indirect immunofluorescence; the presence was confirmed by Western blotting. Clinical data were obtained from medical records, hospital data base, and questionnaire-based direct telephone contact with patients.ResultsAmong 21 patients, aged 54 ± 11, with pulmonary pathologies, the most frequent neurological manifestations were neuropathies. Typical PNS included paraneoplastic cerebellar degeneration (PCD) and limbic encephalitis (LE). We found cases with multiple onconeuronal antibodies (anti-Ri and anti-Yo) and coexisting PNS (PCD/LE). Well-defined onconeuronal antibodies were identified in 23.8% of patients. Among antineuronal antibodies, the most frequent were anti-MAG (23.8%). ROC curves analysis revealed high sensitivity of onconeuronal and antineuronal antibodies for typical PNS and lower for pulmonary malignancies.ConclusionsTests for antibodies are highly sensitive for the diagnosis of typical paraneoplastic neurological syndromes. Anti-myelin and anti-MAG antibodies are associated with non-neoplastic pulmonary diseases. Patients with well-defined onconeuronal antibodies require careful screening and follow-up, because the PNS diagnosis indicates a high probability of an underlying malignancy.

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“…The results suggest that detecting an increase in the blood vessel density of a tumor-draining lymph node may be a potentially promising method for identifying lymph node metastasis at an early stage. However, the model described above has certain limitations: first, because all experimental mice develop metastases to the draining lymph nodes, it is not possible to differentiate draining lymph nodes to which tumor cells do not metastasize from the upstream node; and second, the autoimmune diseases that develop in MRL/lpr mice may induce angiogenesis 26. The growth of lymphatic vessels induced by a tumor (tumor-lymphangiogenesis) is deeply associated with the dissemination of cancer cells through the lymphatic system 27.…”

Section: Introductionmentioning

confidence: 99%

Monitoring of Blood Vessel Density Using Contrast-Enhanced High Frequency Ultrasound May Facilitate Early Diagnosis of Lymph Node Metastasis

et al. 2017

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Time-dependent alterations in the ultrasonography characteristics of lymph nodes during early-stage metastasis have not been compared with those of tumor-draining lymph nodes that do not develop tumor; this is partly due to the absence of an appropriate experimental model. In a previous study of lymph nodes with experimental early-stage metastasis, we used contrast-enhanced high-frequency ultrasound to demonstrate that an increase in lymph node blood vessel density preceded any changes in lymph node volume. In the present study, we used an experimental model of lymph node metastasis in which tumor cells metastasized from the subiliac lymph node to the proper axillary lymph node (the tumor-draining lymph node). We utilized contrast-enhanced high-frequency ultrasound to perform a longitudinal analysis of tumor-draining lymph nodes, comparing those at an early-stage of metastasis with those that did not develop detectable metastasis. We found that the normalized blood vessel density of an early-stage metastatic lymph node exhibited a progressive rise, whereas that of a tumor-draining lymph node not containing tumor began to increase later. For both types of lymph nodes, the normalized blood vessel density on the final day of experiments showed a trend towards being higher than that measured in controls. We further found that mice with an initially low value for lymph node blood vessel density subsequently showed a larger increase in the blood vessel density of the metastatic lymph node; this differed significantly from measurements in controls. The present study indicates that a longitudinal analysis of the blood vessel densities of tumor-draining lymph nodes, made using contrast-enhanced high-frequency ultrasound imaging, may be a potentially promising method for detecting early-stage lymph node metastasis in selected patients. Furthermore, our findings suggest that tumor in an upstream lymph node may induce alteration of the vascular structures in draining lymph nodes that do not contain tumor.

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Angiogenic Activity of Sera from Extrinsic Allergic Alveolitis Patients in Relation to Clinical, Radiological, and Functional Pulmonary Changes

Cited by 7 publications

References 27 publications

Angiogenic Activity of Sera from Pulmonary Tuberculosis Patients in Relation to IL-12p40 and TNFα Serum Levels

Angiogenic Activity of Sera from Pulmonary Tuberculosis Patients in Relation to IL-12p40 and TNFα Serum Levels

Onconeuronal and antineuronal antibodies in patients with neoplastic and non-neoplastic pulmonary pathologies and suspected for paraneoplastic neurological syndrome

Monitoring of Blood Vessel Density Using Contrast-Enhanced High Frequency Ultrasound May Facilitate Early Diagnosis of Lymph Node Metastasis

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