Angiogenic bFGF expression from gas-plasma treated implants

Bailey, Steven R.; Polan, Jodie; Morse, Brian S.; Wetherhold, Suzanne; Villanueva-Vedia, Rosa E.; Waggoner, Douglas; Phelix, Clyde F.; Barera-Roderiquiz, Edwin; Goswami, Nilesh; Munoz, Oscar C.; Agrawal, C. Mauli

doi:10.1016/s1522-1865(03)00098-2

Cited by 13 publications

(4 citation statements)

References 6 publications

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“…Image-Pro Plus, Media Cybernetics UK) and public domain (NIH Image, ImageJ) image analysis software packages permit rapid morphometric analysis of 'particles' within a digital image imported from a wide range of sources. ImageJ (Rasband, 1997(Rasband, -2005 was selected for its ready availability, extensive use in previous medical and scientific research (Bailey et al, 2002;Brown et al, 2005;Cho and Daniel, 2005 accessible user interface and versatility for future extended applications through the use of plug-ins.…”

Section: Combined Automated and Semi-automated Methodsmentioning

confidence: 99%

Practical nerve morphometry

Urso-Baiarda

Grobbelaar

2006

Journal of Neuroscience Methods

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Section: Combined Automated and Semi-automated Methodsmentioning

confidence: 99%

Practical nerve morphometry

Urso-Baiarda

Grobbelaar

2006

Journal of Neuroscience Methods

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“…L ‐PLA is a biodegradable polymer and the gas plasma treatment significantly enhances its oxygen content. We have previously shown that such treatment results in a significant angiogenic, biochemical response from endothelial cells seeded on treated polymer constructs [17–19]. Using a protocol approved by the Institutional Animal Use Committee, nine common farm swine (Sous Scroufulae) were anesthetized and the carotid artery isolated.…”

Section: Methodsmentioning

confidence: 99%

“…Our prior investigations [16–18] have included the use of a bioabsorbable levo poly lactic acid ( L ‐PLA) polymer fabricated at our institution into a microporous, three‐dimensional cylindrical pledget, which when endothelially seeded, promoted in vivo angiogenesis in both mouse and rabbit models.…”

Section: Introductionmentioning

confidence: 99%

Implantation of oxygen enhanced, three‐dimensional microporous L‐PLA polymers: A reproducible porcine model of chronic total coronary occlusion

Prosser

Agrawal

Polan

et al. 2006

Cathet Cardio Intervent

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We have hypothesized that oxygen enhanced three-dimensional microporous poly-L-lactic acid (L-PLA) bioabsorbable polymer constructs could be implanted to produce a subacute occlusion in a porcine coronary artery, forming a thrombofibrotic occlusion containing microvascular channels. Chronic total occlusion (CTO) is increasingly prevalent in patients who present for percutaneous interventions. No reproducible animal coronary model simulating human CTOs has previously been developed. Swine coronary arteries were cannulated and a microporous L-PLA polymer pledget was advanced into a preselected segment of coronary. The coronary arteries were angiographically re-imaged at day 3, day 10, and day 28, to document the presence or absence of an occlusion. Histopathology was also performed at each time point to evaluate the lesion characteristics. A novel three-dimensional L-PLA microporous polymer construct, when implanted into porcine coronary arteries, reproducibly results in the development of a CTO at day 3. The histopathology in this porcine coronary model of CTO at day 28 closely mimics human coronary CTO, including the presence of microvascular channels and dense collagen and elastic tissue in the occlusion.

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“…Five weeks later, a follow-up coronary angiography was performed according to the same protocol described above, after which the swine were killed and the heart was excised for histopathologic examination. l-PLA-induced injury l-PLA is a biodegradable polymer known to promote in vivo angiogenic responses by endothelial cells [21][22][23] and to induce thrombofibrotic occlusion in porcine coronary arteries [18]. l-PLA (Resomer ® L210S Evonik Industries AG, Essen, Germany) with an inherent viscosity of 3.3-4.3 dl/g was purchased.…”

Section: Copper Wire Stent-induced Injurymentioning

confidence: 99%