Angiogenic mechanisms of human dental pulp and their relationship with substance P expression in response to occlusal trauma

Caviedes-Bucheli, Javier; Gomez-Sosa, Jose Francisco; Azuero-Holguin, María M; Ormeño-Gomez, M.; Pinto-Pascual, V.; Muñoz, Hugo Roberto

doi:10.1111/iej.12627

Cited by 46 publications

(52 citation statements)

References 63 publications

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“…Mechanisms including endogenous release of substance P, which activate the survived root odontoblasts and increase their biosynthetic activity, have been suggested (Caviedes‐Bucheli et al . ).…”

Section: Discussionmentioning

confidence: 97%

“…Caviedes‐Bucheli et al . () showed that angiogenesis in the amputated dental pulp comprises two processes, either by modulating endothelial cell growth (direct mechanism) or by attracting cells with angiogenic potential to the injury site (indirect mechanism). Formation of new vessels after the breakage of the pre‐existing vasculature is regulated by a variety of signalling molecules, such as growth factors and endogenous pulp substances (Smith & Cooper ).…”

Section: Discussionmentioning

confidence: 99%

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EDTA conditioning of circumpulpal dentine induces dentinogenic events in pulpotomized miniature swine teeth

Tziafas

Kodonas²,

Gogos³

et al. 2018

Int Endodontic J

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Aim To investigate pulp responses after pulpotomy and EDTA conditioning of pulp chamber dentinal walls with or without the placement of a collagenous scaffold in the experimental model of miniature swine teeth. Methodology Forty‐two fully developed permanent premolars and molars of healthy miniature swines were used. After preparation of pulp exposures through Class I cavities, the tissue of the pulp chamber was completely removed. The haemorrhage was controlled, and the root pulp was protected using a polyurethane film. The circumpulpal pulp chamber dentine was treated for 3 min with normal saline (group 1), or 17% EDTA solution (groups 2 and 3). The film was removed, and the pulp chamber cavities were left empty (groups 1 and 2), or filled with swine collagenous sponge (group 3). The access cavities were restored with a Teflon disc and glass ionomer. Teeth were evaluated histo‐morphologically after 10 weeks. Data were compared using the nonparametric Fisher's exact test. Results Teeth after treatment of dentine with saline (group 1) were associated with no or only traces of hard tissue formation along the root canal walls. Atubular tertiary dentine deposition in the form of matrix deposition along root canal walls, or dentine bridge formation at the orifice of root canals or complete pulp canal obliteration, was found after treatment of dentine with EDTA in both experiments (groups 2 and 3). Significantly different types of mineralization in the root canals of groups 2 and 3 were seen (P = 0.001). Tissue changes in the pulp cavity, characterized by soft tissue growth and osteodentine or atubular tertiary dentine formation, were only seen after EDTA conditioning of dentine, in 6.2% of the teeth without scaffold and 64.7% of the teeth with scaffold application. Newly deposited mineralized matrix in the pulp chamber was always in continuation with hard tissue deposited in the root canals. Conclusions The EDTA conditioning of pulp cavity dentinal walls after pulpotomy induced dentinogenic events in the root pulp. Application of collagenous scaffold in the pulp chamber enhanced soft tissue growth and mineralized tissue formation along the treated circumpulpal dentine.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

EDTA conditioning of circumpulpal dentine induces dentinogenic events in pulpotomized miniature swine teeth

Tziafas

Kodonas²,

Gogos³

et al. 2018

Int Endodontic J

View full text Add to dashboard Cite

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“…Dental pulp stem cells (DPSCs) play two important roles in pulp revascularization. First, they contribute to the angiogenic process by secreting proangiogenic factors, including platelet‐derived growth factor AB (PDGF) and vascular endothelial growth factor (VEGF), as a result of hypoxia or injury (Bronckaers et al , Caviedes‐Bucheli et al ). VEGF best induces angiogenesis or vasculogenesis.…”

Section: Discussionmentioning

confidence: 99%

“…PRGF was used in the surgically prepared socket, because it is an autologous scaffold obtained by platelet activation and fibrinogen polymerization, and contains most of the growth factors and proteins involved in angiogenesis (Caviedes‐Bucheli et al ). Several studies have highlighted the anti‐inflammatory and anti‐bacterial properties of this preparation, as well as its capacity to promote key healing processes such as collagen production, cell differentiation and angiogenesis (Anitua et al ,b).…”

Section: Discussionmentioning

confidence: 99%

“…Autotransplanted teeth respond to hypoxia by releasing many signals from the pulp and the bloodstream, such as VEGF, PDGF and bFGF, that guide the cells in the pulp healing process, which, in turn, initiates vascular neoformation and a connection between the healing site and the pre‐existing pulp tissue vessels. This process finally leads to the apposition of tertiary dentine as a repair mechanism (Caviedes‐Bucheli et al , Rombouts et al ).…”

Section: Discussionmentioning

confidence: 99%

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Successful pulp revascularization of an autotransplantated mature premolar with fragile fracture apicoectomy and plasma rich in growth factors: a 3‐year follow‐up

et al. 2019

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Aim This case report demonstrates a positive outcome of the adjuvant use of fragile fracture (FF), which is a technique used to harvest dental pulp stem cells (DPSCs), and platelet‐rich plasma (PRP) in a mandibular premolar (tooth 44) with a completely formed root that was transplanted into a surgically created socket and which maintained pulp vitality and function. Summary After virtual surgical planning, a 3D tooth replica of tooth 44 was fabricated. A surgical socket was created in the position of tooth 14; then, tooth 44 was extracted and the root dentine was abraded using a turbine diamond bur 3 mm from the apex until a circular groove was prepared around the outer circumference of the root; and then, an FF was performed without damaging the pulp tissue. PRP was placed in the socket, after which the donor tooth was inserted in the recipient area. At 2 weeks post‐treatment, orthodontic traction was applied. At 3‐year follow‐up, the tooth had adequate alignment and was asymptomatic. Response to pulp testing was positive, and the presence of pulp canal obliteration was observed as a sign of pulpal healing. Key learning points Autotransplantation is a good alternative for replacing missing teeth, with repair of tissues and pulp revascularization. Revascularization of an autotransplanted mature tooth using the fragile fracture technique and PRP scaffold is a feasible option and might have positive effects on the long‐term outcome of the procedure. Including completely formed teeth as donors in autotransplantation, maintaining vitality and their functions is an option that warrants further study.

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Dentin‐Pulp and Periodontal Anatomy and Physiology

Tjäderhane¹,

Paju²

2019

Essential Endodontology

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Angiogenic mechanisms of human dental pulp and their relationship with substance P expression in response to occlusal trauma

Abstract: Caviedes-Bucheli J, Gomez-Sosa JF, Azuero-Holguin MM, Ormeño-Gomez M, Pinto-Pascual V, Munoz HR.Angiogenic mechanisms of human dental pulp and their relationship with substance P expression in response to occlusal trauma.

Cited by 46 publications

References 63 publications

EDTA conditioning of circumpulpal dentine induces dentinogenic events in pulpotomized miniature swine teeth

EDTA conditioning of circumpulpal dentine induces dentinogenic events in pulpotomized miniature swine teeth

Successful pulp revascularization of an autotransplantated mature premolar with fragile fracture apicoectomy and plasma rich in growth factors: a 3‐year follow‐up

Dentin‐Pulp and Periodontal Anatomy and Physiology

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