Angiogenic potential of boron-containing bioactive glasses: in vitro study

Influence of dissolution products of a novel Ca-enriched silicate bioactive glass-ceramic on VEGF release from bone marrow stromal cells

“…In the context of induced vascularization, granule sizes of more than 2 mm seemed to inhibit the VEGF expression, but this was not the case for smaller granular sizes, for example, 0.5-0.8 mm or 1.0-2.0 mm. A similar assessment of angiogenesis was carried out recently on boron containing bioactive glasses using bone marrow stromal cells [18]. Interestingly, it has been also shown that 500 µg/ml nanosized BG particles enhanced the VEGF expression significantly in osteoclast-like cells after 14 days of cultivation [19].…”

“…In addition to the conventional characterization and testing of biomaterials involving in-vitro cell biology studies which include cell proliferation, adhesion and differentiation studies followed by in-vivo experiments, emphasis must be also given to evaluate in detail and specifically the angiogenic potential of the biomaterial. Quantifying the secretion of VEGF from relevant cells exposed to the dissolution products of biomaterials in-vitro is one of the assays commonly used [17,18]. VEGF is one of the key growth factors and cytokines expressed during the inflammatory response phase.…”

Bioactive glasses and glass‐ceramics versus hydroxyapatite: Comparison of angiogenic potential and biological responsiveness

“…This assay enables the accurate measurement of the recombinant mouse VEGF in cell culture supernatants by engaging an antibody specific for mouse VEGF. The assay was carried out according to the manufacturer's protocol, according to our previous study (Balasubramanian et al, ). Briefly, the extracted cell‐culture supernatants and standards of known VEGF concentrations were transferred to a 96‐well plate, provided with the kit, which is precoated with the mouse antibody, enabling to complex with the bound VEGF antibody for 2.5 hr.…”

“…This assay enables the accurate measurement of the recombinant mouse VEGF in cell culture supernatants by engaging an antibody specific for mouse VEGF. The assay was carried out according to the manufacturer's protocol, according to our previous study (Balasubramanian et al, 2017).…”

Guest editors’ preface

“…Several papers deal with the investigation of novel compositions of silicate and phosphate bioactive glasses, in particular glasses with therapeutic ion delivery capability to enhance cellular response, an area highlighted in one of the most recent papers authored by Larry Hench in 2015 [6], which reflects the importance of this topic for applications of bioactive glasses in regenerative medicine and as antibacterial agent. For example, novel bioactive silicate glasses (both melt-derived and solgel produced) incorporating B [7,8], bivalent ions such as Mg, Zn, Sr and Cu [9], Li [10], Ce [11], Sr [12,13], Cu, Zn [14], Mn [15], are discussed. The release of ''classical'' ions in bioactive glasses, namely Si, Ca, P, is the subject of the studies of Houreh et al [16] and Obata et al [17] in the context of dental pulp stem cells and osteoblast cell responses, respectively.…”